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Literature summary extracted from

  • Takakura, Y.; Kuwata, S.
    Purification, characterization, and molecular cloning of a pyranose oxidase from the fruit body of the basidiomycete,Tricholoma matsutake (2003), Biosci. Biotechnol. Biochem., 67, 2598-2607.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.10
-
 Tricholoma matsutake

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.10 1.28
-
 D-glucose pH 7.0, 37°C Tricholoma matsutake
1.1.3.10 10.7
-
 1,5-anhydro-D-glucitol pH 7.0, 37°C Tricholoma matsutake
1.1.3.10 26.4
-
 L-sorbose pH 7.0, 37°C Tricholoma matsutake
1.1.3.10 45
-
 D-galactose pH 7.0, 37°C Tricholoma matsutake
1.1.3.10 45.8
-
 D-xylose pH 7.0, 37°C Tricholoma matsutake
1.1.3.10 174
-
 D-arabinose pH 7.0, 37°C Tricholoma matsutake
1.1.3.10 192
-
 trehalose pH 7.0, 37°C Tricholoma matsutake
1.1.3.10 295
-
 D-mannose pH 7.0, 37°C Tricholoma matsutake
1.1.3.10 329
-
 D-maltose pH 7.0, 37°C Tricholoma matsutake

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.10 62000
-
 4 * 62000, SDS-PAGE Tricholoma matsutake
1.1.3.10 250000
-
 gel filtration Tricholoma matsutake

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.10 Tricholoma matsutake
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.10
-
 Tricholoma matsutake

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.10 fruit body
-
 Tricholoma matsutake
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.10 25.8
-
-
 Tricholoma matsutake

Storage Stability

EC Number Storage Stability Organism
1.1.3.10 -20°C, 0.1 M Tris-HCl, pH 7.0, enzyme retains 78% of the initial activity after 6 months in absence of glycerol Tricholoma matsutake
1.1.3.10 4°C, pH 7.0, enzyme retains more than 90% of the activity Tricholoma matsutake

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.10 1,5-anhydro-D-glucitol + O2 18.4% of the activity with D-glucose Tricholoma matsutake ?
-
 ?
1.1.3.10 D-arabinose + O2 1.87% of the activity with D-glucose Tricholoma matsutake ?
-
 ?
1.1.3.10 D-galactose + O2 5.41% of the activity with D-glucose Tricholoma matsutake ?
-
 ?
1.1.3.10 D-glucose + O2
-
 Tricholoma matsutake D-glucosone + H2O2
-
 ?
1.1.3.10 D-maltose + O2 15.1% of the activity with D-glucose Tricholoma matsutake ?
-
 ?
1.1.3.10 D-mannose + O2 6.02% of the activity with D-glucose Tricholoma matsutake ?
-
 ?
1.1.3.10 D-xylose + O2 13.4% of the activity with D-glucose Tricholoma matsutake ?
-
 ?
1.1.3.10 L-sorbose + O2 17.9% of the activity with D-glucose Tricholoma matsutake ?
-
 ?
1.1.3.10 trehalose + O2 14.3% of the activity with D-glucose Tricholoma matsutake ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.10 tetramer 4 * 62000, SDS-PAGE Tricholoma matsutake

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.10 50
-
-
 Tricholoma matsutake

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.10 30 70 30°C: about 65% of maximal activity, 70°C: about 35% of maximal activity Tricholoma matsutake

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.10 30
-
 30 min, stable Tricholoma matsutake
1.1.3.10 50
-
 30 min, about 5% loss of activity Tricholoma matsutake
1.1.3.10 55
-
 30 min, about 40% loss of activity Tricholoma matsutake
1.1.3.10 60
-
 30 min, about 45% loss of activity Tricholoma matsutake
1.1.3.10 65
-
 30 min, about 60% loss of activity Tricholoma matsutake
1.1.3.10 70
-
 30 min, about 65% loss of activity Tricholoma matsutake

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.10 7.79
-
 D-arabinose pH 7.0, 37°C Tricholoma matsutake
1.1.3.10 22.5
-
 D-galactose pH 7.0, 37°C Tricholoma matsutake
1.1.3.10 25.1
-
 D-mannose pH 7.0, 37°C Tricholoma matsutake
1.1.3.10 55.8
-
 D-xylose pH 7.0, 37°C Tricholoma matsutake
1.1.3.10 59.6
-
 trehalose pH 7.0, 37°C Tricholoma matsutake
1.1.3.10 62.9
-
 D-maltose pH 7.0, 37°C Tricholoma matsutake
1.1.3.10 74.6
-
 L-sorbose pH 7.0, 37°C Tricholoma matsutake
1.1.3.10 76.7
-
 1,5-anhydro-D-glucitol pH 7.0, 37°C Tricholoma matsutake
1.1.3.10 111
-
 D-glucose pH 7.0, 37°C Tricholoma matsutake

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.10 7.5 8
-
 Tricholoma matsutake

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.10 5.5 11 pH 5.5: about 40% of maximal activity, pH 11: about 40% of maximal activity Tricholoma matsutake

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.10 4.5
-
 37°C, 30 min, 60% loss of activity Tricholoma matsutake
1.1.3.10 5.5
-
 37°C, 30 min, about 15% loss of activity Tricholoma matsutake
1.1.3.10 7 11 37°C, 30 min, stable Tricholoma matsutake