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Literature summary extracted from

  • Ohgi, K.; Kudo, S.; Takeuchi, M.; Iwama, M.; Irie, M.
    Enzymatic properties of phenylalanine 101 mutant enzyme of ribonuclease Rh from Rhizopus niveus (2000), Biosci. Biotechnol. Biochem., 64, 2068-2074.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.19 expression of mutant enzymes in Saccharomyces cerevisiae R27-7C-1C, secretion to culture medium Rhizopus niveus

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.19 F101A site-directed mutagenesis, below 1% activity compared to the wild-type enzyme Rhizopus niveus
4.6.1.19 F101I site-directed mutagenesis, 3.8% activity compared to the wild-type enzyme Rhizopus niveus
4.6.1.19 F101K site-directed mutagenesis, below 1% activity compared to the wild-type enzyme Rhizopus niveus
4.6.1.19 F101L site-directed mutagenesis, 20% activity compared to the wild-type enzyme Rhizopus niveus
4.6.1.19 F101Q site-directed mutagenesis, below 1% activity compared to the wild-type enzyme Rhizopus niveus
4.6.1.19 F101W site-directed mutagenesis, 7% activity compared to the wild-type enzyme Rhizopus niveus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.19 additional information
-
 additional information Km values for mutant enzymes with diverse dinucleotide substrates, overview Rhizopus niveus
4.6.1.19 0.045
-
 GpG wild-type enzyme, pH 5.0, 23°C Rhizopus niveus
4.6.1.19 0.049
-
 CpC wild-type enzyme, pH 5.0, 23°C Rhizopus niveus
4.6.1.19 0.052
-
 ApG wild-type enzyme, pH 5.0, 23°C Rhizopus niveus
4.6.1.19 0.063
-
 UpG wild-type enzyme, pH 5.0, 23°C Rhizopus niveus
4.6.1.19 0.065
-
 ApA wild-type enzyme, pH 5.0, 23°C Rhizopus niveus
4.6.1.19 0.068
-
 UpC wild-type enzyme, pH 5.0, 23°C Rhizopus niveus
4.6.1.19 0.072
-
 GpA wild-type enzyme, pH 5.0, 23°C Rhizopus niveus
4.6.1.19 0.076
-
 CpU wild-type enzyme, pH 5.0, 23°C Rhizopus niveus
4.6.1.19 0.078
-
 ApU wild-type enzyme, pH 5.0, 23°C Rhizopus niveus
4.6.1.19 0.079
-
 GpU wild-type enzyme, pH 5.0, 23°C Rhizopus niveus
4.6.1.19 0.11
-
 GpC wild-type enzyme, pH 5.0, 23°C Rhizopus niveus
4.6.1.19 0.11
-
 CpG wild-type enzyme, pH 5.0, 23°C Rhizopus niveus
4.6.1.19 0.13
-
 ApC wild-type enzyme, pH 5.0, 23°C Rhizopus niveus
4.6.1.19 0.13
-
 CpA wild-type enzyme, pH 5.0, 23°C Rhizopus niveus
4.6.1.19 0.13
-
 UpU wild-type enzyme, pH 5.0, 23°C Rhizopus niveus
4.6.1.19 0.14
-
 UpA wild-type enzyme, pH 5.0, 23°C Rhizopus niveus

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.19 Rhizopus niveus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.19 recombinant mutant enzymes from culture supernatant of transformed Saccharomyces cerevisiae Rhizopus niveus

Reaction

EC Number Reaction Comment Organism Reaction ID
4.6.1.19 RNA + H2O = an [RNA fragment]-3'-nucleoside-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] Phe101 is important for catalytic activity Rhizopus niveus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.19 0.18
-
 purified recombinant mutant F101K Rhizopus niveus
4.6.1.19 0.7
-
 purified recombinant mutant F101R Rhizopus niveus
4.6.1.19 7.8
-
 purified recombinant mutant F101A Rhizopus niveus
4.6.1.19 8.6
-
 purified recombinant mutant F101Q Rhizopus niveus
4.6.1.19 60
-
 purified recombinant mutant F101I Rhizopus niveus
4.6.1.19 110
-
 purified recombinant mutant F101W Rhizopus niveus
4.6.1.19 340
-
 purified recombinant mutant F101L Rhizopus niveus
4.6.1.19 1600
-
 purified wild-type enzyme Rhizopus niveus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.19 ApA + H2O
-
 Rhizopus niveus AMP + adenosine
-
 ?
4.6.1.19 ApC + H2O
-
 Rhizopus niveus AMP + cytosine
-
 ?
4.6.1.19 ApG + H2O
-
 Rhizopus niveus AMP + guanosine
-
 ?
4.6.1.19 ApU + H2O
-
 Rhizopus niveus AMP + uridine
-
 ?
4.6.1.19 CpA + H2O
-
 Rhizopus niveus CMP + adenosine
-
 ?
4.6.1.19 CpC + H2O
-
 Rhizopus niveus CMP + cytidine
-
 ?
4.6.1.19 CpG + H2O
-
 Rhizopus niveus CMP + guanosine
-
 ?
4.6.1.19 CpU + H2O
-
 Rhizopus niveus 3'-CMP + uridine
-
 ?
4.6.1.19 GpA + H2O
-
 Rhizopus niveus GMP + adenosine
-
 ?
4.6.1.19 GpC + H2O
-
 Rhizopus niveus GMP + cytosine
-
 ?
4.6.1.19 GpG + H2O best substrate Rhizopus niveus GMP + guanosine
-
 ?
4.6.1.19 GpU + H2O
-
 Rhizopus niveus GMP + uridine
-
 ?
4.6.1.19 additional information subsite base specificities at the B1 and B2 site of wild-type and mutant enzymes, overview Rhizopus niveus ?
-
 ?
4.6.1.19 RNA + H2O
-
 Rhizopus niveus ?
-
 ?
4.6.1.19 UpA + H2O
-
 Rhizopus niveus UMP + adenosine
-
 ?
4.6.1.19 UpC + H2O
-
 Rhizopus niveus UMP + cytosine
-
 ?
4.6.1.19 UpG + H2O
-
 Rhizopus niveus UMP + guanosine
-
 ?
4.6.1.19 UpU + H2O
-
 Rhizopus niveus UMP + uridine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.19 base-nonspecific RNase Rh
-
 Rhizopus niveus
4.6.1.19 More enzyme belongs to the ribonuclease T2 family Rhizopus niveus
4.6.1.19 RNase Rh
-
 Rhizopus niveus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.6.1.19 additional information
-
 assay temperatures depending on substrate Rhizopus niveus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.19 5
-
 assay at Rhizopus niveus