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Literature summary extracted from

  • Kashiwabara, S.I.; Azuma, S.; Tsuduki, M.; Suzuki, Y.
    The primary structure of the subunit in Bacillus thermoamyloliquefaciens KP1071 molecular weight 540,000 homohexameric alpha-glucosidase II belonging to the glycosyl hydrolase family 31 (2000), Biosci. Biotechnol. Biochem., 64, 1379-1393.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.20 construction of a genomic library, gene mapping, DNA and amino acid sequence determination and analysis, expression in Escherichia coli HB101 and DH5alpha Bacillus thermoamyloliquefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 90000
-
 6 * 91070, alpha-glucosidase II, sequence calculation, 6 * 90000, alpha-glucosidase II, SDS-PAGE Bacillus thermoamyloliquefaciens
3.2.1.20 91070
-
 6 * 91070, alpha-glucosidase II, sequence calculation, 6 * 90000, alpha-glucosidase II, SDS-PAGE Bacillus thermoamyloliquefaciens
3.2.1.20 530000
-
 alpha-glucosidase II, native PAGE Bacillus thermoamyloliquefaciens
3.2.1.20 540000
-
 alpha-glucosidase II, gel filtration Bacillus thermoamyloliquefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Bacillus thermoamyloliquefaciens Q9F234 3 alpha-glucosidases I, II, and III
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 recombinant alpha-glucosidase II from Escherichia coli, 86fold Bacillus thermoamyloliquefaciens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.20 maltotetraose + 3 H2O = 4 alpha-D-glucose the enzyme has both exo-alpha-1,4-glucosidase and oligo-alpha-1,6-glucosidase activities Bacillus thermoamyloliquefaciens
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 64.7
-
 purified alpha-glucosidase II Bacillus thermoamyloliquefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 isomaltose + H2O hydrolysis of alpha-1,6-bond Bacillus thermoamyloliquefaciens 2 D-glucose
-
 ?
3.2.1.20 maltose + H2O hydrolysis of alpha-1,4-bond Bacillus thermoamyloliquefaciens 2 D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 hexamer 6 * 91070, alpha-glucosidase II, sequence calculation, 6 * 90000, alpha-glucosidase II, SDS-PAGE Bacillus thermoamyloliquefaciens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.20 More enzyme belongs to the family 31 of glycosyl hydrolases Bacillus thermoamyloliquefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 80
-
 alpha-glucosidase II Bacillus thermoamyloliquefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 70
-
 alpha-glucosidase II, stable up to for 10 min Bacillus thermoamyloliquefaciens
3.2.1.20 80
-
 alpha-glucosidase II, half-life 10 min Bacillus thermoamyloliquefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 6.8
-
 assay at Bacillus thermoamyloliquefaciens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.20 Bacillus thermoamyloliquefaciens alpha-glucosidase II, isoelectric focusing
-
 4.9