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Literature summary extracted from

  • Banks, J.; Cane, D.E.
    Biosynthesis of vitamin B6: direct identification of the product of the PdxA-catalyzed oxidation of 4-hydroxy-L-threonine-4-phosphate using electrospray ionization mass spectrometry (2004), Bioorg. Med. Chem. Lett., 14, 1633-16367.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.262 Zn2+ tightly bound, divalent metal ion-dependent enzyme, Zn2+ can be replaced by e.g. Mg2+ Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.262 4-(phosphonooxy)threonine + NAD(P)+ Escherichia coli involved in the biosynthesis of vitamin B6 3-amino-1-hydroxyacetone 1-phosphate + CO2 + NAD(P)H
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.262 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.262 4-phosphooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH + H+ 3-amino-1-hydroxyacetone 1-phosphate,i.e. AHAB, is the primary product of the reaction, no formation of 2-amino-3-oxo-4-phosphonooxybutyrate as intermediate is detected, identification by electrospray ionization mass spectrometry Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.262 4-(phosphonooxy)threonine + NAD(P)+ involved in the biosynthesis of vitamin B6 Escherichia coli 3-amino-1-hydroxyacetone 1-phosphate + CO2 + NAD(P)H
-
 ?
1.1.1.262 4-(phosphonooxy)threonine + NAD(P)+ i.e. 4-hydroxy-L-threonine-4-phosphate, HTP Escherichia coli 3-amino-1-hydroxyacetone 1-phosphate + CO2 + NAD(P)H i.e. AHAB, primary product of the reaction, no formation of 2-amino-3-oxo-4-phosphonooxybutyrate as intermediate is detected, identification by electrospray ionization mass spectrometry ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.262 PdxA
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.262 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.262 7.5
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.262 NAD(P)+ dependent on Escherichia coli