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Literature summary extracted from

  • Boselli, A.; Piubelli, L.; Molla, G.; Sacchi, S.; Pilone, M.S.; Ghisla, S.; Pollegioni, L.
    On the mechanism of Rhodotorula gracilis D-amino acid oxidase: role of the active site serine 335 (2004), Biochim. Biophys. Acta, 1702, 19-32.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.3 S335G mutant enzyme has a lower turnover number with D-Ala than wild-type enzyme. The spectral and ligand binding properties of the mutant are similar to those of the wild-type enzyme Rhodotorula toruloides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 0.25
-
 O2 pH 8.5, 25°C, mutant enzyme S335G, reaction with D-Ala Rhodotorula toruloides
1.4.3.3 0.8
-
 D-Ala pH 8.5, 25°C, wild-type enzyme, at Rhodotorula toruloides
1.4.3.3 1.1
-
 D-Ala pH 8.5, 25°C, mutant enzyme S335G Rhodotorula toruloides
1.4.3.3 2.3
-
 O2 pH 8.5, 25°C, wild-type enzyme, reaction with D-Ala Rhodotorula toruloides
1.4.3.3 18
-
 D-Asp pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides
1.4.3.3 30
-
 D-Asp pH 8.5, 25°C, mutant enzyme S335G Rhodotorula toruloides

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Rhodotorula toruloides
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-Ala + H2O + O2
-
 Rhodotorula toruloides pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-Asp + H2O + O2
-
 Rhodotorula toruloides oxaloacetate + NH3 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.3 RgDAAO
-
 Rhodotorula toruloides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.3 82
-
 O2 pH 8.5, 25°C, mutant enzyme S335G Rhodotorula toruloides
1.4.3.3 82
-
 D-Ala pH 8.5, 25°C, mutant enzyme S335G Rhodotorula toruloides
1.4.3.3 350
-
 O2 pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides
1.4.3.3 350
-
 D-Ala pH 8.5, 25°C, wild-type enzyme Rhodotorula toruloides