Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ichiyama, S.; Kurihara, T.; Kogure, Y.; Tsunasawa, S.; Kawasaki, H.; Esaki, N.
    Reactivity of asparagine residue at the active site of the D105N mutant of fluoroacetate dehalogenase from Moraxella sp. B (2004), Biochim. Biophys. Acta, 1698, 27-36.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.3 expression of wild-type and mutant enzymes in Escherichia coli Moraxella sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.8.1.3 D105N site-directed mutagenesis, catalytic site mutant, shows reduced activity, but reactivation occurs due to hydrolytic deamination of the mutant Asn105 to the wild-type Asp105, reactivation in a time- and temperature-dependent manner Moraxella sp.

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.3 Moraxella sp.
-
-
-
3.8.1.3 Moraxella sp. B
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.3 recombinant wild-type and mutant enzymes from Escherichia coli Moraxella sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
3.8.1.3 haloacetate + H2O = glycolate + halide As105 is a catalytic site residue functioning as a nucleophil attacking the alpha-carbon atom of the substrate to displace the fluorine atom, catalytic mechanism, possibly involving an imidate as intermediate Moraxella sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.3 chloroacetate + H2O
-
 Moraxella sp. glycolate + chloride
-
 ?
3.8.1.3 chloroacetate + H2O
-
 Moraxella sp. B glycolate + chloride
-
 ?
3.8.1.3 fluoroacetate + H2O very high dissociation energy of the carbon-fluorine bond Moraxella sp. glycolate + fluoride
-
 ?
3.8.1.3 fluoroacetate + H2O very high dissociation energy of the carbon-fluorine bond Moraxella sp. B glycolate + fluoride
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.8.1.3 Fac-DEX
-
 Moraxella sp.
3.8.1.3 fluoroacetate dehalogenase
-
 Moraxella sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.8.1.3 30
-
 assay at Moraxella sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.3 9.5
-
 assay at Moraxella sp.