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Literature summary extracted from

  • Abdelghany, H.M.; Gasmi, L.; Cartwright, J.L.; Bailey, S.; Rafferty, J.B.; McLennan, A.G.
    Cloning, characterisation and crystallization of a diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase from Caenorhabditis elegans (2001), Biochim. Biophys. Acta, 1550, 27-36.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.17 expression in Escherichia coli Caenorhabditis elegans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.17 sitting-drop vapour diffusion, crystals diffract to a minimum d-spacing of 2 A and belong to either space group C222 or C222(1) Caenorhabditis elegans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.17 adenosine 5'-tetraphosphate competitive Caenorhabditis elegans
3.6.1.17 F- non-competitive Caenorhabditis elegans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.17 0.007
-
 Ap4A pH 7.8, 25°C Caenorhabditis elegans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.17 Co2+ divalent cation required, 20% of maximal activity in presence of 1 mM Co2+ Caenorhabditis elegans
3.6.1.17 Mg2+ divalent cation required, maximal activity in presence of 3-5 mM Mg2+ Caenorhabditis elegans
3.6.1.17 Mn2+ divalent cation required, 20% of maximal activity in presence of 0.1 mM Mn2+ Caenorhabditis elegans
3.6.1.17 Zn2+ divalent cation required, 20% of maximal activity in presence of 0.05 mM Mn2+ Caenorhabditis elegans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.17 19000
-
 gel filtration Caenorhabditis elegans

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.17 Caenorhabditis elegans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.17 Ap4A + H2O
-
 Caenorhabditis elegans ?
-
 ?
3.6.1.17 Ap6A + H2O 7% of the activity with Ap4A Caenorhabditis elegans ATP + ATP
-
 ?
3.6.1.17 diadenosine 5',5''-P1,P4-tetraphosphate + H2O
-
 Caenorhabditis elegans ATP + AMP
-
 ?
3.6.1.17 diadenosine 5',5''-P1,P5-pentaphosphate + H2O 19% of the activity with Ap4A Caenorhabditis elegans ATP + ADP
-
 ?
3.6.1.17 additional information no activity with Ap3A Caenorhabditis elegans ?
-
 ?
3.6.1.17 p4A + H2O 14% of the activity with Ap4A Caenorhabditis elegans ATP + phosphate
-
 ?
3.6.1.17 p5A + H2O 29% of the activity with Ap4A Caenorhabditis elegans ATP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.17 Ap4A hydrolase
-
 Caenorhabditis elegans
3.6.1.17 diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase
-
 Caenorhabditis elegans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.17 27
-
 Ap4A pH 7.8, 25°C Caenorhabditis elegans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.17 7.3
-
 hydrolysis pf Ap4A Caenorhabditis elegans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.17 6.5 8 50% of maximal activity at pH 6.5 and at pH 8.0, hydrolysis of Ap4A Caenorhabditis elegans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.17 0.00001
-
 adenosine 5'-tetraphosphate pH 7.8, 25°C Caenorhabditis elegans
3.6.1.17 0.025
-
 F- pH 7.8, 25°C Caenorhabditis elegans