BRENDA - Enzyme Database

Cloning, characterisation and crystallization of a diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase from Caenorhabditis elegans

Abdelghany, H.M.; Gasmi, L.; Cartwright, J.L.; Bailey, S.; Rafferty, J.B.; McLennan, A.G.; Biochim. Biophys. Acta 1550, 27-36 (2001)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.6.1.17
expression in Escherichia coli
Caenorhabditis elegans
Crystallization (Commentary)
EC Number
Crystallization
Organism
3.6.1.17
sitting-drop vapour diffusion, crystals diffract to a minimum d-spacing of 2 A and belong to either space group C222 or C222(1)
Caenorhabditis elegans
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.17
adenosine 5'-tetraphosphate
competitive
Caenorhabditis elegans
3.6.1.17
F-
non-competitive
Caenorhabditis elegans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.007
-
Ap4A
pH 7.8, 25°C
Caenorhabditis elegans
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.17
Co2+
divalent cation required, 20% of maximal activity in presence of 1 mM Co2+
Caenorhabditis elegans
3.6.1.17
Mg2+
divalent cation required, maximal activity in presence of 3-5 mM Mg2+
Caenorhabditis elegans
3.6.1.17
Mn2+
divalent cation required, 20% of maximal activity in presence of 0.1 mM Mn2+
Caenorhabditis elegans
3.6.1.17
Zn2+
divalent cation required, 20% of maximal activity in presence of 0.05 mM Mn2+
Caenorhabditis elegans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.17
19000
-
gel filtration
Caenorhabditis elegans
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.17
Caenorhabditis elegans
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
Ap6A + H2O
7% of the activity with Ap4A
654817
Caenorhabditis elegans
ATP + ATP
-
-
-
?
3.6.1.17
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
-
654817
Caenorhabditis elegans
ATP + AMP
-
-
-
?
3.6.1.17
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
19% of the activity with Ap4A
654817
Caenorhabditis elegans
ATP + ADP
-
-
-
?
3.6.1.17
additional information
no activity with Ap3A
654817
Caenorhabditis elegans
?
-
-
-
-
3.6.1.17
p4A + H2O
14% of the activity with Ap4A
654817
Caenorhabditis elegans
ATP + phosphate
-
-
-
?
3.6.1.17
p5A + H2O
29% of the activity with Ap4A
654817
Caenorhabditis elegans
ATP + diphosphate
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.17
27
-
Ap4A
pH 7.8, 25°C
Caenorhabditis elegans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.17
7.3
-
hydrolysis pf Ap4A
Caenorhabditis elegans
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.6.1.17
6.5
8
50% of maximal activity at pH 6.5 and at pH 8.0, hydrolysis of Ap4A
Caenorhabditis elegans
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.6.1.17
0.00001
-
adenosine 5'-tetraphosphate
pH 7.8, 25°C
Caenorhabditis elegans
3.6.1.17
0.025
-
F-
pH 7.8, 25°C
Caenorhabditis elegans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.17
expression in Escherichia coli
Caenorhabditis elegans
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.6.1.17
sitting-drop vapour diffusion, crystals diffract to a minimum d-spacing of 2 A and belong to either space group C222 or C222(1)
Caenorhabditis elegans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.17
adenosine 5'-tetraphosphate
competitive
Caenorhabditis elegans
3.6.1.17
F-
non-competitive
Caenorhabditis elegans
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.6.1.17
0.00001
-
adenosine 5'-tetraphosphate
pH 7.8, 25°C
Caenorhabditis elegans
3.6.1.17
0.025
-
F-
pH 7.8, 25°C
Caenorhabditis elegans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.007
-
Ap4A
pH 7.8, 25°C
Caenorhabditis elegans
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.17
Co2+
divalent cation required, 20% of maximal activity in presence of 1 mM Co2+
Caenorhabditis elegans
3.6.1.17
Mg2+
divalent cation required, maximal activity in presence of 3-5 mM Mg2+
Caenorhabditis elegans
3.6.1.17
Mn2+
divalent cation required, 20% of maximal activity in presence of 0.1 mM Mn2+
Caenorhabditis elegans
3.6.1.17
Zn2+
divalent cation required, 20% of maximal activity in presence of 0.05 mM Mn2+
Caenorhabditis elegans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.17
19000
-
gel filtration
Caenorhabditis elegans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
Ap6A + H2O
7% of the activity with Ap4A
654817
Caenorhabditis elegans
ATP + ATP
-
-
-
?
3.6.1.17
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
-
654817
Caenorhabditis elegans
ATP + AMP
-
-
-
?
3.6.1.17
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
19% of the activity with Ap4A
654817
Caenorhabditis elegans
ATP + ADP
-
-
-
?
3.6.1.17
additional information
no activity with Ap3A
654817
Caenorhabditis elegans
?
-
-
-
-
3.6.1.17
p4A + H2O
14% of the activity with Ap4A
654817
Caenorhabditis elegans
ATP + phosphate
-
-
-
?
3.6.1.17
p5A + H2O
29% of the activity with Ap4A
654817
Caenorhabditis elegans
ATP + diphosphate
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.17
27
-
Ap4A
pH 7.8, 25°C
Caenorhabditis elegans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.17
7.3
-
hydrolysis pf Ap4A
Caenorhabditis elegans
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.6.1.17
6.5
8
50% of maximal activity at pH 6.5 and at pH 8.0, hydrolysis of Ap4A
Caenorhabditis elegans