BRENDA - Enzyme Database

Regulation of NAD(+)-dependent isocitrate dehydrogenase in the citrate producing yeast Yarrowia lipolytica

Morgunov, I.G.; Solodovnikova, N.Y.; Sharyshev, A.A.; Kamzolova, S.V.; Finogenova, T.V.; Biochemistry 69, 1391-1398 (2004)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.41
AMP
-
Yarrowia lipolytica
General Stability
EC Number
General Stability
Organism
1.1.1.41
20% glycerol stabilizes during storage
Yarrowia lipolytica
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.41
glyoxylate
slight inhibition
Yarrowia lipolytica
1.1.1.41
NADH
complete inhibition at 0.2 mM, competitive
Yarrowia lipolytica
1.1.1.41
oxaloacetate
slight inhibition
Yarrowia lipolytica
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.136
-
NAD+
pH 7.4, 25°C
Yarrowia lipolytica
1.1.1.41
0.581
-
isocitrate
pH 7.4, 25°C
Yarrowia lipolytica
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.41
Mg2+
-
Yarrowia lipolytica
1.1.1.42
Mg2+
-
Yarrowia lipolytica
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.41
52000
-
8 * 52000, SDS-PAGE
Yarrowia lipolytica
1.1.1.41
412000
-
gel filtration
Yarrowia lipolytica
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Yarrowia lipolytica
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
additional information
Yarrowia lipolytica
enzyme is involved in tricarboxylic acid cycle and plays a key role in the regulation of biosynthesis of citrate and isocitrate, yeast is overproducing citrate under nitrogen deficiency in presence of glucose
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.41
Yarrowia lipolytica
-
citrate-overproducing yeast, strain BKM Y-2373
-
1.1.1.42
Yarrowia lipolytica
-
citrate-overproducing yeast
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.41
129fold to homogeneity by ammonium sulfate fractionation, hydrophobic chromatography on an octyl resin, and gel filtration
Yarrowia lipolytica
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.41
0.15
-
enzyme extract of cells in exponential growth phase
Yarrowia lipolytica
1.1.1.41
0.16
-
enzyme extract of cells in stationary growth phase
Yarrowia lipolytica
1.1.1.41
0.17
-
enzyme extract of cells in growth phase with acid synthesis
Yarrowia lipolytica
1.1.1.41
22
-
purified enzyme
Yarrowia lipolytica
1.1.1.42
0.24
-
enzyme extract of cells in stationary growth phase
Yarrowia lipolytica
1.1.1.42
0.26
-
enzyme extract of cells in growth phase with acid synthesis
Yarrowia lipolytica
1.1.1.42
0.3
-
enzyme extract of cells in exponential growth phase
Yarrowia lipolytica
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
654783
Yarrowia lipolytica
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
additional information
enzyme is involved in tricarboxylic acid cycle and plays a key role in the regulation of biosynthesis of citrate and isocitrate, yeast is overproducing citrate under nitrogen deficiency in presence of glucose
654783
Yarrowia lipolytica
?
-
-
-
-
1.1.1.42
isocitrate + NADP+
-
654783
Yarrowia lipolytica
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.41
octamer
8 * 52000, SDS-PAGE
Yarrowia lipolytica
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.41
25
-
assay at
Yarrowia lipolytica
1.1.1.42
25
-
assay at
Yarrowia lipolytica
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.41
7.4
-
-
Yarrowia lipolytica
1.1.1.42
9
-
assay at
Yarrowia lipolytica
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
-
Yarrowia lipolytica
1.1.1.42
NADP+
dependent on
Yarrowia lipolytica
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.41
0.063
-
NADH
pH 7.4, 25°C
Yarrowia lipolytica
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.41
AMP
-
Yarrowia lipolytica
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
-
Yarrowia lipolytica
1.1.1.42
NADP+
dependent on
Yarrowia lipolytica
General Stability (protein specific)
EC Number
General Stability
Organism
1.1.1.41
20% glycerol stabilizes during storage
Yarrowia lipolytica
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.41
glyoxylate
slight inhibition
Yarrowia lipolytica
1.1.1.41
NADH
complete inhibition at 0.2 mM, competitive
Yarrowia lipolytica
1.1.1.41
oxaloacetate
slight inhibition
Yarrowia lipolytica
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.41
0.063
-
NADH
pH 7.4, 25°C
Yarrowia lipolytica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.136
-
NAD+
pH 7.4, 25°C
Yarrowia lipolytica
1.1.1.41
0.581
-
isocitrate
pH 7.4, 25°C
Yarrowia lipolytica
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.41
Mg2+
-
Yarrowia lipolytica
1.1.1.42
Mg2+
-
Yarrowia lipolytica
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.41
52000
-
8 * 52000, SDS-PAGE
Yarrowia lipolytica
1.1.1.41
412000
-
gel filtration
Yarrowia lipolytica
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Yarrowia lipolytica
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
additional information
Yarrowia lipolytica
enzyme is involved in tricarboxylic acid cycle and plays a key role in the regulation of biosynthesis of citrate and isocitrate, yeast is overproducing citrate under nitrogen deficiency in presence of glucose
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.41
129fold to homogeneity by ammonium sulfate fractionation, hydrophobic chromatography on an octyl resin, and gel filtration
Yarrowia lipolytica
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.41
0.15
-
enzyme extract of cells in exponential growth phase
Yarrowia lipolytica
1.1.1.41
0.16
-
enzyme extract of cells in stationary growth phase
Yarrowia lipolytica
1.1.1.41
0.17
-
enzyme extract of cells in growth phase with acid synthesis
Yarrowia lipolytica
1.1.1.41
22
-
purified enzyme
Yarrowia lipolytica
1.1.1.42
0.24
-
enzyme extract of cells in stationary growth phase
Yarrowia lipolytica
1.1.1.42
0.26
-
enzyme extract of cells in growth phase with acid synthesis
Yarrowia lipolytica
1.1.1.42
0.3
-
enzyme extract of cells in exponential growth phase
Yarrowia lipolytica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
654783
Yarrowia lipolytica
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
additional information
enzyme is involved in tricarboxylic acid cycle and plays a key role in the regulation of biosynthesis of citrate and isocitrate, yeast is overproducing citrate under nitrogen deficiency in presence of glucose
654783
Yarrowia lipolytica
?
-
-
-
-
1.1.1.42
isocitrate + NADP+
-
654783
Yarrowia lipolytica
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.41
octamer
8 * 52000, SDS-PAGE
Yarrowia lipolytica
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.41
25
-
assay at
Yarrowia lipolytica
1.1.1.42
25
-
assay at
Yarrowia lipolytica
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.41
7.4
-
-
Yarrowia lipolytica
1.1.1.42
9
-
assay at
Yarrowia lipolytica