EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.41 | AMP | - |
Yarrowia lipolytica |
EC Number | General Stability | Organism |
---|---|---|
1.1.1.41 | 20% glycerol stabilizes during storage | Yarrowia lipolytica |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.41 | glyoxylate | slight inhibition | Yarrowia lipolytica | |
1.1.1.41 | NADH | complete inhibition at 0.2 mM, competitive | Yarrowia lipolytica | |
1.1.1.41 | oxaloacetate | slight inhibition | Yarrowia lipolytica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.41 | 0.136 | - |
NAD+ | pH 7.4, 25°C | Yarrowia lipolytica | |
1.1.1.41 | 0.581 | - |
isocitrate | pH 7.4, 25°C | Yarrowia lipolytica |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.41 | Mg2+ | - |
Yarrowia lipolytica | |
1.1.1.42 | Mg2+ | - |
Yarrowia lipolytica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.41 | 52000 | - |
8 * 52000, SDS-PAGE | Yarrowia lipolytica |
1.1.1.41 | 412000 | - |
gel filtration | Yarrowia lipolytica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.41 | isocitrate + NAD+ | Yarrowia lipolytica | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
1.1.1.41 | additional information | Yarrowia lipolytica | enzyme is involved in tricarboxylic acid cycle and plays a key role in the regulation of biosynthesis of citrate and isocitrate, yeast is overproducing citrate under nitrogen deficiency in presence of glucose | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.41 | Yarrowia lipolytica | - |
citrate-overproducing yeast, strain BKM Y-2373 | - |
1.1.1.42 | Yarrowia lipolytica | - |
citrate-overproducing yeast | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.41 | 129fold to homogeneity by ammonium sulfate fractionation, hydrophobic chromatography on an octyl resin, and gel filtration | Yarrowia lipolytica |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.41 | 0.15 | - |
enzyme extract of cells in exponential growth phase | Yarrowia lipolytica |
1.1.1.41 | 0.16 | - |
enzyme extract of cells in stationary growth phase | Yarrowia lipolytica |
1.1.1.41 | 0.17 | - |
enzyme extract of cells in growth phase with acid synthesis | Yarrowia lipolytica |
1.1.1.41 | 22 | - |
purified enzyme | Yarrowia lipolytica |
1.1.1.42 | 0.24 | - |
enzyme extract of cells in stationary growth phase | Yarrowia lipolytica |
1.1.1.42 | 0.26 | - |
enzyme extract of cells in growth phase with acid synthesis | Yarrowia lipolytica |
1.1.1.42 | 0.3 | - |
enzyme extract of cells in exponential growth phase | Yarrowia lipolytica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.41 | isocitrate + NAD+ | - |
Yarrowia lipolytica | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
1.1.1.41 | additional information | enzyme is involved in tricarboxylic acid cycle and plays a key role in the regulation of biosynthesis of citrate and isocitrate, yeast is overproducing citrate under nitrogen deficiency in presence of glucose | Yarrowia lipolytica | ? | - |
? | |
1.1.1.42 | isocitrate + NADP+ | - |
Yarrowia lipolytica | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.41 | octamer | 8 * 52000, SDS-PAGE | Yarrowia lipolytica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.41 | NAD+-dependent isocitrate dehydrogenase | - |
Yarrowia lipolytica |
1.1.1.42 | NADP+-dependent isocitrate dehydrogenase | - |
Yarrowia lipolytica |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.41 | 25 | - |
assay at | Yarrowia lipolytica |
1.1.1.42 | 25 | - |
assay at | Yarrowia lipolytica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.41 | 7.4 | - |
- |
Yarrowia lipolytica |
1.1.1.42 | 9 | - |
assay at | Yarrowia lipolytica |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.41 | NAD+ | - |
Yarrowia lipolytica | |
1.1.1.42 | NADP+ | dependent on | Yarrowia lipolytica |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.41 | 0.063 | - |
NADH | pH 7.4, 25°C | Yarrowia lipolytica |