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Literature summary extracted from

  • Brown, W.M.; Yowell, C.A.; Hoard, A.; vander Jagt, T.A.; Hunsaker, L.A.; Deck, L.M.; Royer, R.E.; Piper, R.C.; Dame, J.B.; Makler, M.T.; vander Jagt, D.L.
    Comparative structural analysis and kinetic properties of lactate dehydrogenases from the four species of human Malarial parasites (2004), Biochemistry, 43, 6219-6229.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.27 medicine potential drug target for new antimalarials Plasmodium malariae
1.1.1.27 medicine potential drug target for new antimalarials Plasmodium ovale
1.1.1.27 medicine potential drug target for new antimalarials Plasmodium vivax
1.1.1.27 medicine potential drug target for new antimalarials Plasmodium falciparum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.27 expression in Escherichia coli Plasmodium malariae
1.1.1.27 expression in Escherichia coli Plasmodium ovale
1.1.1.27 expression in Escherichia coli Plasmodium vivax
1.1.1.27 expression in Escherichia coli Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.27 gossylic nitrile 1,1'-diacetate
-
 Plasmodium falciparum
1.1.1.27 gossylic nitrile 1,1'-diacetate
-
 Plasmodium malariae
1.1.1.27 gossylic nitrile 1,1'-diacetate
-
 Plasmodium ovale
1.1.1.27 gossylic nitrile 1,1'-diacetate
-
 Plasmodium vivax
1.1.1.27 gossypol
-
 Plasmodium falciparum
1.1.1.27 gossypol
-
 Plasmodium malariae
1.1.1.27 gossypol
-
 Plasmodium ovale
1.1.1.27 gossypol
-
 Plasmodium vivax
1.1.1.27 gossypol lactone
-
 Plasmodium falciparum
1.1.1.27 gossypol lactone
-
 Plasmodium malariae
1.1.1.27 gossypol lactone
-
 Plasmodium ovale
1.1.1.27 gossypol lactone
-
 Plasmodium vivax

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.27 0.007
-
 NADH pH 7.5, 25°C Plasmodium vivax
1.1.1.27 0.007
-
 NADH pH 7.5, 25°C Plasmodium falciparum
1.1.1.27 0.009
-
 NADH pH 7.5, 25°C Plasmodium malariae
1.1.1.27 0.014
-
 NADH pH 7.5, 25°C Plasmodium ovale
1.1.1.27 0.017
-
 pyruvate pH 7.5, 25°C Plasmodium vivax
1.1.1.27 0.03
-
 pyruvate pH 7.5, 25°C Plasmodium falciparum
1.1.1.27 0.071
-
 pyruvate pH 7.5, 25°C Plasmodium ovale
1.1.1.27 0.086
-
 NAD+ pH 9.2, 25°C Plasmodium falciparum
1.1.1.27 0.123
-
 3-acetylpyridine adenine dinucleotide pH 9.2, 25°C Plasmodium falciparum
1.1.1.27 0.14
-
 pyruvate pH 7.5, 25°C Plasmodium malariae
1.1.1.27 0.143
-
 NAD+ pH 9.2, 25°C Plasmodium malariae
1.1.1.27 0.155
-
 NAD+ pH 9.2, 25°C Plasmodium vivax
1.1.1.27 0.168
-
 3-acetylpyridine adenine dinucleotide pH 9.2, 25°C Plasmodium malariae
1.1.1.27 0.182
-
 3-acetylpyridine adenine dinucleotide pH 9.2, 25°C Plasmodium vivax
1.1.1.27 0.311
-
 NAD+ pH 9.2, 25°C Plasmodium ovale
1.1.1.27 0.408
-
 3-acetylpyridine adenine dinucleotide pH 9.2, 25°C Plasmodium ovale
1.1.1.27 10.2
-
 (S)-lactate pH 9.2, 25°C Plasmodium vivax
1.1.1.27 11.3
-
 (S)-lactate pH 9.2, 25°C Plasmodium malariae
1.1.1.27 12
-
 (S)-lactate pH 9.2, 25°C Plasmodium falciparum
1.1.1.27 93
-
 (S)-lactate pH 9.2, 25°C Plasmodium ovale

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Plasmodium falciparum Q27743
-
-
1.1.1.27 Plasmodium malariae
-
-
-
1.1.1.27 Plasmodium ovale
-
-
-
1.1.1.27 Plasmodium vivax Q6JH30
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.27
-
 Plasmodium malariae
1.1.1.27
-
 Plasmodium ovale
1.1.1.27
-
 Plasmodium vivax
1.1.1.27
-
 Plasmodium falciparum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.27 215
-
 reduction of pyruvate Plasmodium ovale
1.1.1.27 216
-
 reduction of pyruvate Plasmodium malariae
1.1.1.27 253
-
 reduction of pyruvate Plasmodium falciparum
1.1.1.27 450
-
 reduction of pyruvate Plasmodium vivax

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 (S)-lactate + 3-acetylpyridine adenine dinucleotide
-
 Plasmodium malariae pyruvate + reduced 3-acetylpyridine adenine dinucleotide
-
 ?
1.1.1.27 (S)-lactate + 3-acetylpyridine adenine dinucleotide
-
 Plasmodium ovale pyruvate + reduced 3-acetylpyridine adenine dinucleotide
-
 ?
1.1.1.27 (S)-lactate + 3-acetylpyridine adenine dinucleotide
-
 Plasmodium vivax pyruvate + reduced 3-acetylpyridine adenine dinucleotide
-
 ?
1.1.1.27 (S)-lactate + 3-acetylpyridine adenine dinucleotide
-
 Plasmodium falciparum pyruvate + reduced 3-acetylpyridine adenine dinucleotide
-
 ?
1.1.1.27 pyruvate + NADH + H+
-
 Plasmodium malariae (S)-lactate + NAD+
-
 r
1.1.1.27 pyruvate + NADH + H+
-
 Plasmodium ovale (S)-lactate + NAD+
-
 r
1.1.1.27 pyruvate + NADH + H+
-
 Plasmodium vivax (S)-lactate + NAD+
-
 r
1.1.1.27 pyruvate + NADH + H+
-
 Plasmodium falciparum (S)-lactate + NAD+
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.27 12.33
-
 NAD+ pH 9.2, 25°C Plasmodium vivax
1.1.1.27 12.33
-
 (S)-lactate pH 9.2, 25°C Plasmodium vivax
1.1.1.27 25
-
 NAD+ pH 9.2, 25°C Plasmodium ovale
1.1.1.27 25
-
 (S)-lactate pH 9.2, 25°C Plasmodium ovale
1.1.1.27 38.33
-
 NAD+ pH 9.2, 25°C Plasmodium malariae
1.1.1.27 38.33
-
 (S)-lactate pH 9.2, 25°C Plasmodium malariae
1.1.1.27 65
-
 NAD+ pH 9.2, 25°C Plasmodium falciparum
1.1.1.27 65
-
 (S)-lactate pH 9.2, 25°C Plasmodium falciparum
1.1.1.27 125
-
 NADH pH 7.5, 25°C Plasmodium malariae
1.1.1.27 125
-
 pyruvate pH 7.5, 25°C Plasmodium malariae
1.1.1.27 126.7
-
 NADH pH 7.5, 25°C Plasmodium vivax
1.1.1.27 126.7
-
 pyruvate pH 7.5, 25°C Plasmodium vivax
1.1.1.27 148.3
-
 NADH pH 7.5, 25°C Plasmodium falciparum
1.1.1.27 148.3
-
 pyruvate pH 7.5, 25°C Plasmodium falciparum
1.1.1.27 153.3
-
 3-acetylpyridine adenine dinucleotide pH 9.2, 25°C Plasmodium ovale
1.1.1.27 233.3
-
 3-acetylpyridine adenine dinucleotide pH 9.2, 25°C Plasmodium malariae
1.1.1.27 250
-
 3-acetylpyridine adenine dinucleotide pH 9.2, 25°C Plasmodium falciparum
1.1.1.27 266.7
-
 NADH pH 7.5, 25°C Plasmodium ovale
1.1.1.27 266.7
-
 pyruvate pH 7.5, 25°C Plasmodium ovale
1.1.1.27 333.3
-
 3-acetylpyridine adenine dinucleotide pH 9.2, 25°C Plasmodium vivax

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.27 NAD+
-
 Plasmodium malariae
1.1.1.27 NAD+
-
 Plasmodium ovale
1.1.1.27 NAD+
-
 Plasmodium vivax
1.1.1.27 NAD+
-
 Plasmodium falciparum
1.1.1.27 NADH
-
 Plasmodium malariae
1.1.1.27 NADH
-
 Plasmodium ovale
1.1.1.27 NADH
-
 Plasmodium vivax
1.1.1.27 NADH
-
 Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.27 0.00007
-
 gossypol lactone pH 7.5, 25°C Plasmodium vivax
1.1.1.27 0.00024
-
 gossylic nitrile 1,1'-diacetate pH 7.5, 25°C Plasmodium vivax
1.1.1.27 0.0004
-
 gossypol lactone pH 7.5, 25°C Plasmodium falciparum
1.1.1.27 0.0005
-
 gossypol lactone pH 7.5, 25°C Plasmodium ovale
1.1.1.27 0.0007
-
 gossypol pH 7.5, 25°C Plasmodium falciparum
1.1.1.27 0.0007
-
 gossylic nitrile 1,1'-diacetate pH 7.5, 25°C Plasmodium ovale
1.1.1.27 0.0007
-
 gossylic nitrile 1,1'-diacetate pH 7.5, 25°C Plasmodium falciparum
1.1.1.27 0.0014
-
 gossypol pH 7.5, 25°C Plasmodium vivax
1.1.1.27 0.0015
-
 gossypol lactone pH 7.5, 25°C Plasmodium malariae
1.1.1.27 0.0018
-
 gossylic nitrile 1,1'-diacetate pH 7.5, 25°C Plasmodium malariae
1.1.1.27 0.0019
-
 gossypol pH 7.5, 25°C Plasmodium ovale
1.1.1.27 0.0126
-
 gossypol pH 7.5, 25°C Plasmodium malariae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.27 Plasmodium vivax
-
-
 6
1.1.1.27 Plasmodium falciparum
-
-
 6.5
1.1.1.27 Plasmodium malariae
-
-
 6.9
1.1.1.27 Plasmodium ovale
-
-
 8