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Literature summary extracted from

  • Valley, M.P.; Fitzpatrick, P.F.
    Reductive half-reaction of nitroalkane oxidase: effect of mutation of the active site apartate to glutamate (2003), Biochemistry, 42, 5850-5856.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.3.1 additional information enzyme induction by growth on nitroethane Fusarium oxysporum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.3.1 recombinant expression of wild-type and mutant D402E enzymes Fusarium oxysporum

Protein Variants

EC Number Protein Variants Comment Organism
1.7.3.1 D402E site-directed mutagenesis, altered kinetics compared to the wild-type enzyme, mutation has no effect on the rate of reaction of the reduced enzyme with oxygen, reaction mechanism analysis Fusarium oxysporum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.3.1 additional information no substrate inhibition Fusarium oxysporum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.3.1 additional information
-
 additional information steady-state and rapid reaction kinetics of wild-type and mutant enzymes Fusarium oxysporum
1.7.3.1 0.03
-
 1-nitrobutane recombinant wild-type enzyme, pH 8.0, 30°C Fusarium oxysporum
1.7.3.1 0.18
-
 1-nitrobutane recombinant mutant D403E, pH 8.0, 30°C Fusarium oxysporum
1.7.3.1 2.3
-
 nitroethane recombinant wild-type enzyme, pH 8.0, 30°C Fusarium oxysporum
1.7.3.1 14.6
-
 nitroethane recombinant mutant D403E, pH 8.0, 30°C Fusarium oxysporum

Organism

EC Number Organism UniProt Comment Textmining
1.7.3.1 Fusarium oxysporum
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.7.3.1 ethylnitronate + O2 + FMNH2 = acetaldehyde + nitrite + FMN + H2O Asp402 acts as the active site base of the enzyme, mechanism, substrate CH-bond cleavage is rate limiting for the reduction of the enzyme by nitroethane Fusarium oxysporum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.3.1 1-nitrobutane + O2 + H2O
-
 Fusarium oxysporum butyraldehyde + nitrite + H2O2
-
 ?
1.7.3.1 additional information enzyme catalyzes the oxidation of primary and secondary nitroalkanes to the respective aldehydes or ketones, releasing nitrite Fusarium oxysporum ?
-
 ?
1.7.3.1 nitroethane + O2 + H2O O2 is delivered from air-saturated buffer in the assay reaction Fusarium oxysporum ethanal + nitrite + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.3.1 More enzyme belongs to the acyl-CoA dehydrogenase family of enzymes Fusarium oxysporum
1.7.3.1 NAO
-
 Fusarium oxysporum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.3.1 30
-
 assay at Fusarium oxysporum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.3.1 8
-
 assay at Fusarium oxysporum

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.3.1 FAD
-
 Fusarium oxysporum