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Literature summary extracted from
- Bacterial expression,characterization, and disulfide bond determination of soluble human NTPDase6 (CD39L2) nucleotidase: implications for structure and function (2003), Biochemistry, 42, 11726-11735.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.6.1.6 | expression in Escherichia coli BL21(DE3), refolding of the enzyme from bacterial inclusion bodies | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.6.1.6 | C139S | mutant enzyme lacking the single free sulfhydryl is more stable than the wild-type enzyme under refolding conditions, 10-15°C, 2-3 days. The mutant enzyme is less susceptible to oxidative inactivation than the wild-type enzyme | Homo sapiens |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.6.1.6 | mutant enzyme C139S lacking the single free sulfhydryl is more stable than the wild-type enzyme under refolding conditions, 10-15°C, 2-3 days | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.6 | iodoacetamide | - |
Homo sapiens |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.6 | 0.13 | - |
GDP | in presence of Ca2+, wild-type and mutant enzyme | Homo sapiens | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.6 | Ca2+ | activates, slightly more effective than Mg2+ | Homo sapiens | |
| 3.6.1.6 | Mg2+ | activates, slightly less effective than Ca2+ | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.6 | 43000 | - |
x * 43000, SDS-PAGE | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.6.1.6 | Homo sapiens | O75354 | - |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 3.6.1.6 | the mutant enzyme C139S is less susceptible to oxidative inactivation than the wild-type enzyme | Homo sapiens |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.6.1.6 | glycoprotein | nevertheless the bacterially expressed refolded enzyme is not glycosylated, it has high specific activity, indicating that glycosylation is not essential for folding or nucleotidase activity of NTPDase6 | Homo sapiens |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.6.1.6 | - |
Homo sapiens |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.6.1.6 | refolding of the enzyme from bacterial inclusion bodies. For wild-type enzyme optimal refolding ocurs at 10-15°C over 2-3 days | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.6.1.6 | brain | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.6 | ADP + H2O | 24.4% of the specific activity with GDP | Homo sapiens | AMP + phosphate | - |
? | |
| 3.6.1.6 | CDP + H2O | 12.9% of the specific activity with GDP | Homo sapiens | CMP + phosphate | - |
? | |
| 3.6.1.6 | CTP + H2O | 3.8% of the specific activity with GDP | Homo sapiens | CDP + phosphate | - |
? | |
| 3.6.1.6 | GDP + H2O | best substrate | Homo sapiens | GMP + phosphate | - |
? | |
| 3.6.1.6 | GTP + H2O | 4.4% of the specific activity with GDP | Homo sapiens | GDP + phosphate | - |
? | |
| 3.6.1.6 | IDP + H2O | 55.5% of the specific activity with GDP | Homo sapiens | IMP + phosphate | - |
? | |
| 3.6.1.6 | ITP + H2O | 6% of the specific activity with GDP | Homo sapiens | IDP + phosphate | - |
? | |
| 3.6.1.6 | additional information | no activity with ATP | Homo sapiens | ? | - |
? | |
| 3.6.1.6 | UDP + H2O | 19.8% of the specific activity with GDP | Homo sapiens | UMP + phosphate | - |
? | |
| 3.6.1.6 | UTP + H2O | 1.2% of the specific activity with GDP | Homo sapiens | UDP + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.6.1.6 | ? | x * 43000, SDS-PAGE | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.6.1.6 | NTPDase6 | - |
Homo sapiens |
| 3.6.1.6 | NTPDase6 (CD39L2) nucleotidase | - |
Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.6 | 7 | 7.4 | - |
Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.6 | 6.7 | 7.8 | pH 6.7: about 50% of maximal activity with Mg2+ or Ca2+, about 80% of maximal activity with Ca2+, about 70% of maximal activity with Mg2+ | Homo sapiens |
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