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Literature summary extracted from

  • Cheng, H.L.; Tsai, L.C.; Lin, S.S.; Yuan, H.S.; Yang, N.S.; Lee, S.H.; Shyur, L.F.
    Mutagenesis of Trp(54) and Trp(203) residues on Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase significantly affects catalytic activities of the enzyme (2002), Biochemistry, 41, 8759-8766.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.73 W105F significant decrease in thermostability Fibrobacter succinogenes
3.2.1.73 W105H significant decrease in thermostability Fibrobacter succinogenes
3.2.1.73 W141F 5-7-fold increase in KM-value for lichenan compared to wild type, decrease in kcat-value, no significant change in thermal stability Fibrobacter succinogenes
3.2.1.73 W141H 5-7-fold increase in KM-value for lichenan compared to wild type, decrease in kcat-value, no significant change in thermal stability Fibrobacter succinogenes
3.2.1.73 W148F decrease in kcat-value, no significant change in thermal stability Fibrobacter succinogenes
3.2.1.73 W165F after incubation at pH 3.0, 1 h, 3-7-fold higher activity than wild type Fibrobacter succinogenes
3.2.1.73 W165H significant decrease in thermostability Fibrobacter succinogenes
3.2.1.73 W186F increase in kcat-value, no significant change in thermal stability Fibrobacter succinogenes
3.2.1.73 W198F significant decrease in thermostability Fibrobacter succinogenes
3.2.1.73 W203F increase in kcat-value, no significant change in thermal stability Fibrobacter succinogenes
3.2.1.73 W203R 5-7-fold increase in KM-value for lichenan compared to wild type, decrease in kcat-value, after incubation at pH 3.0, 1 h, 3-7-fold higher activity than wild type, no significant change in thermal stability Fibrobacter succinogenes
3.2.1.73 W54F decrease in kcat-value, no significant change in thermal stability Fibrobacter succinogenes
3.2.1.73 W54Y decrease in kcat-value, no significant change in thermal stability Fibrobacter succinogenes

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Fibrobacter succinogenes
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 15 19 pH 6.0, mutant W105H Fibrobacter succinogenes
3.2.1.73 29
-
 pH 6.0, mutant W54F Fibrobacter succinogenes
3.2.1.73 37
-
 pH 6.0, mutant W54Y Fibrobacter succinogenes
3.2.1.73 69
-
 pH 6.0, mutant W141H Fibrobacter succinogenes
3.2.1.73 70
-
 pH 6.0, mutant W203R Fibrobacter succinogenes
3.2.1.73 419
-
 pH 6.0, mutant W141F Fibrobacter succinogenes
3.2.1.73 480
-
 pH 6.0, mutant W148F Fibrobacter succinogenes
3.2.1.73 916
-
 pH 6.0, mutant W112F Fibrobacter succinogenes
3.2.1.73 1572
-
 pH 6.0, mutant W165H Fibrobacter succinogenes
3.2.1.73 1621
-
 pH 6.0, mutant W105F Fibrobacter succinogenes
3.2.1.73 1755
-
 pH 6.0, mutant W198F Fibrobacter succinogenes
3.2.1.73 1791
-
 pH 6.0, mutant W165F Fibrobacter succinogenes
3.2.1.73 2065
-
 pH 6.0, wild type Fibrobacter succinogenes
3.2.1.73 2941
-
 pH 6.0, mutant W186F Fibrobacter succinogenes
3.2.1.73 8726
-
 pH 6.0, mutant W203F Fibrobacter succinogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 lichenan + H2O
-
 Fibrobacter succinogenes ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.73 50
-
 unstable above Fibrobacter succinogenes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.73 5 10
-
 Fibrobacter succinogenes