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Literature summary extracted from

  • Vallmitjana, M.; Ferrer-Navarro, M.; Planell, R.; Abel, M.; Ausin, C.; Querol, E.; Planas, A.; Perez-Pons, J.A.
    Mechanism of the family 1 beta-glucosidase from Streptomyces sp: catalytic residues and kinetic studies (2001), Biochemistry, 40, 5975-5982.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expression in Escherichia coli Streptomyces sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.21 E178A 3500fold reduction in catalytic efficiency, E178 ist the general acid/base catalyst of Bgl3 Streptomyces sp.
3.2.1.21 E178Q 250fold reduction in catalytic efficiency Streptomyces sp.
3.2.1.21 E383A 100000fold reduction in catalytic efficiency Streptomyces sp.
3.2.1.21 E383Q 1000000fold reduction in catalytic efficiency, E383 is the catalytic nucleophile of Bgl3 Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.008
-
 2,4-dinitrophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
3.2.1.21 0.015
-
 2,3-dinitrophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
3.2.1.21 0.023
-
 2-nitrophenyl beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
3.2.1.21 0.054
-
 3,5-dinitrophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
3.2.1.21 0.058
-
 5,11-Dimethyl-6H-pyrido[4,3-b]-carbazole 30°C, pH 6.5 Streptomyces sp.
3.2.1.21 0.064
-
 3,4-dinitrophenyl beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
3.2.1.21 0.1
-
 p-nitrophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
3.2.1.21 0.14
-
 p-nitrophenyl-beta-D-fucopyranoside 50°C, pH 6.5 Streptomyces sp.
3.2.1.21 0.15
-
 p-nitrophenyl-beta-D-glucopyranoside 50°C, pH 6.5 Streptomyces sp.
3.2.1.21 0.24
-
 3-nitrophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
3.2.1.21 0.47
-
 4-bromophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
3.2.1.21 2.3
-
 phenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
3.2.1.21 4.1
-
 cellobiose 50°C, pH 6.5 Streptomyces sp.
3.2.1.21 7.3
-
 p-nitrophenyl-beta-D-galactopyranoside 50°C, pH 6.5 Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 cellobiose + H2O Streptomyces sp.
-
 2 beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Streptomyces sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 recombinant Bgl3 Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 2,3-dinitrophenyl-beta-D-glucopyranoside + H2O
-
 Streptomyces sp. 2,3-dinitrophenol + beta-D-glucose
-
 ?
3.2.1.21 2,4-dinitrophenyl-beta-D-glucopyranoside + H2O
-
 Streptomyces sp. 2,4-dinitrophenol + beta-D-glucose
-
 ?
3.2.1.21 2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Streptomyces sp. 2-nitrophenol + beta-D-glucopyranose
-
 ?
3.2.1.21 3,4-dinitrophenyl beta-D-glucopyranoside + H2O
-
 Streptomyces sp. 3,4-dinitrophenol + beta-D-glucose
-
 ?
3.2.1.21 3,4-dinitrophenyl beta-D-glucopyranoside + H2O
-
 Streptomyces sp. 3,4-dinitrophenol + beta-D-glucopyranose
-
 ?
3.2.1.21 3,5-dinitrophenyl beta-D-glucopyranoside + H2O
-
 Streptomyces sp. 3,5-dinitrophenol + D-glucose
-
 ?
3.2.1.21 3-nitrophenyl beta-D-glucopyranoside + H2O
-
 Streptomyces sp. 3-nitrophenol + D-glucose
-
 ?
3.2.1.21 4-bromophenyl-beta-D-glucopyranoside + H2O
-
 Streptomyces sp. 4-bromophenol + beta-D-glucose
-
 ?
3.2.1.21 4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Streptomyces sp. 4-methylumbelliferone + beta-D-glucose
-
 ?
3.2.1.21 5,11-dimethyl-6H-pyrido[4,3-b]-carbazole + H2O
-
 Streptomyces sp. ?
-
 ?
3.2.1.21 cellobiose + H2O
-
 Streptomyces sp. 2 beta-D-glucose
-
 ?
3.2.1.21 p-nitrophenyl-beta-D-fucopyranoside + H2O
-
 Streptomyces sp. p-nitrophenol + beta-D-fucose
-
 ?
3.2.1.21 p-nitrophenyl-beta-D-galactopyranoside + H2O
-
 Streptomyces sp. p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.21 p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Streptomyces sp. p-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 phenyl-beta-D-glucpyranoside + H2O
-
 Streptomyces sp. phenol + beta-D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 Bgl3
-
 Streptomyces sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.21 30 55
-
 Streptomyces sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 0.66
-
 phenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
3.2.1.21 3.32
-
 4-bromophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
3.2.1.21 7.72
-
 2-nitrophenyl beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
3.2.1.21 8.14
-
 p-nitrophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
3.2.1.21 11.7
-
 4-methylumbelliferyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
3.2.1.21 11.7
-
 2,3-dinitrophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
3.2.1.21 15.8
-
 3-nitrophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
3.2.1.21 18.2
-
 3,4-dinitrophenyl beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
3.2.1.21 20.5
-
 3,5-dinitrophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
3.2.1.21 28.4
-
 p-nitrophenyl-beta-D-glucopyranoside 50°C, pH 6.5 Streptomyces sp.
3.2.1.21 30.4
-
 2,4-dinitrophenyl-beta-D-glucopyranoside 30°C, pH 6.5 Streptomyces sp.
3.2.1.21 35.6
-
 cellobiose 50°C, pH 6.5 Streptomyces sp.
3.2.1.21 37.1
-
 p-nitrophenyl-beta-D-fucopyranoside 50°C, pH 6.5 Streptomyces sp.
3.2.1.21 118
-
 p-nitrophenyl-beta-D-galactopyranoside 50°C, pH 6.5 Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6.5
-
-
 Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 5.5 7.5 approx. 505 of maximal activity at pH 5.5, approx. 25% of maximal activity at pH 7.0 Streptomyces sp.