EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.6.1.6 | K464A | mutant of the NBF1+R segment (nucleotide binding domain 1 and regulatory domain), Vmax is reduced about 50% | Homo sapiens |
5.6.1.6 | K464H | mutant of the NBF1+R segment (nucleotide binding domain 1 and regulatory domain), Vmax is reduced about 50% | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.6.1.6 | Cd2+ | - |
Homo sapiens | |
5.6.1.6 | vanadate | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.6.1.6 | 0.0087 | - |
ATP | 37°C, reaction with the the NBF1+R segment (nucleotide binding domain 1 and regulatory domain) | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.6.1.6 | Co2+ | best divalent metal activator | Homo sapiens | |
5.6.1.6 | Mg2+ | activates | Homo sapiens | |
5.6.1.6 | Mn2+ | activates | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.6.1.6 | ATP + H2O + closed Cl- channel | Homo sapiens | - |
ADP + phosphate + open Cl- channel | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.6.1.6 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.6.1.6 | the NBF1+R segment (nucleotide binding domain 1 and regulatory domain) and its mutant forms K464H and K464A | Homo sapiens |
EC Number | Renatured (Comment) | Organism |
---|---|---|
5.6.1.6 | the guanidine hydrochloride-solubilized and denatured wild-type and mutant NBF1+R proteins can be renatured at 25°C upon rapid dilution with a buffered solution containing glycerol | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.6.1.6 | ATP + H2O + closed Cl- channel | - |
Homo sapiens | ADP + phosphate + open Cl- channel | - |
? | |
5.6.1.6 | ATP + H2O + closed Cl- channel | the NBF1+R segment (nucleotide binding domain 1 and regulatory domain) has the capacity to catalyze ATP hydrolysis in the absence of a fusion partner | Homo sapiens | ADP + phosphate + open Cl- channel | - |
? |