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Literature summary extracted from

  • Becker, D.; Johnson, K.S.; Koivula, A.; Schulein, M.; Sinnott, M.L.
    Hydrolyses of alpha- and beta-cellobiosyl fluorides by Cel6A (cellobiohydrolase II) of Trichoderma reesei and Humicola insolens (2000), Biochem. J., 345, 315-319.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.91 0.022
-
 beta-cellobiosyl fluoride pH 5, 20°C Humicola insolens
3.2.1.91 0.15
-
 beta-cellobiosyl fluoride pH 5, 20°C Trichoderma reesei
3.2.1.91 0.77
-
 alpha-cellobiosyl fluoride pH 5, 20°C Humicola insolens
3.2.1.91 2.35
-
 alpha-cellobiosyl fluoride pH 5, 20°C Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.91 Humicola insolens
-
-
-
3.2.1.91 Trichoderma reesei
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.91 alpha-cellobiosyl fluoride + H2O
-
 Humicola insolens fluoride + cellobiose
-
 ?
3.2.1.91 alpha-cellobiosyl fluoride + H2O
-
 Trichoderma reesei fluoride + cellobiose
-
 ?
3.2.1.91 beta-cellobiosyl fluoride + H2O
-
 Humicola insolens fluoride + cellobiose
-
 ?
3.2.1.91 beta-cellobiosyl fluoride + H2O
-
 Trichoderma reesei fluoride + cellobiose
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.91 0.01
-
 alpha-cellobiosyl fluoride pH 5, 20°C Humicola insolens
3.2.1.91 0.071
-
 beta-cellobiosyl fluoride pH 5, 20°C Humicola insolens
3.2.1.91 0.54
-
 alpha-cellobiosyl fluoride pH 5, 20°C Trichoderma reesei
3.2.1.91 4
-
 beta-cellobiosyl fluoride pH 5, 20°C Trichoderma reesei