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Literature summary extracted from

  • Han, C.; Wang, Q.; Dong, L.; Sun, H.; Peng, S.; Chen, J.; Yang, Y.; Yue, J.; Shen, X.; Jiang, H.
    Molecular cloning and characterization of a new peptide deformylase from human pathogenic bacterium Helicobacter pylori (2004), Biochem. Biophys. Res. Commun., 319, 1292-1298.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.88 expression in Escherichia coli Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.88 1.7
-
 formyl-Met-Ala-Ser pH 7.5, 37°C, Co2+-containing enzyme Helicobacter pylori

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.88 Co2+ the activity of Co2+-containing enzyme is higher than the activity of Zn2+-containing enzyme Helicobacter pylori
3.5.1.88 Zn2+ the activity of Co2+-containing enzyme is higher than the activity of Zn2+-containing enzyme Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.88 20970
-
 x * 20970, mass spectrometry Helicobacter pylori
3.5.1.88 20973
-
 x * 20973, calculation from nucleotide sequence Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.88 Helicobacter pylori Q672W7
-
-
3.5.1.88 Helicobacter pylori SS1 Q672W7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.88
-
 Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.88 formyl-Met-Ala-Ser + H2O
-
 Helicobacter pylori formate + Met-Ala-Ser
-
 ?
3.5.1.88 formyl-Met-Ala-Ser + H2O
-
 Helicobacter pylori SS1 formate + Met-Ala-Ser
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.88 ? x * 20970, mass spectrometry Helicobacter pylori
3.5.1.88 ? x * 20973, calculation from nucleotide sequence Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
3.5.1.88 HpPDF
-
 Helicobacter pylori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.88 50
-
-
 Helicobacter pylori

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.88 40 60 40°C: about 50% of maximal activity, 60°C, about 70% of maximal activity Helicobacter pylori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.88 3.4
-
 formyl-Met-Ala-Ser pH 7.5, 37°C, Co2+-containing enzyme Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.88 8 9
-
 Helicobacter pylori

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.88 6.5 11 pH 6.5: about 55% of maximal activity, pH 11.0: about 90% of maximal activity Helicobacter pylori