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Literature summary extracted from

  • Ishikura, S.; Usami, N.; El-Kabbani, O.; Hara, A.
    Structural determinant for cold inactivation of rodent L-xylulose reductase (2003), Biochem. Biophys. Res. Commun., 308, 68-72.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.10 expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.10 crystal structure analysis and modelling Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.10 D238E site-directed mutagenesis, mutant exists in dimeric form at low temperature like the wild-type enzyme resulting in cold inactivation Mus musculus
1.1.1.10 D238E/L242W site-directed mutagenesis, mutation leads to complete prevention of cold inactivation, mutant exists in tetrameric form at low temperature Mus musculus
1.1.1.10 D238E/L242W/T244C site-directed mutagenesis, double mutation leads to partial prevention of cold inactivation, mutant exists in dimeric and tetrameric form at low temperature Mus musculus
1.1.1.10 L242W site-directed mutagenesis, mutation leads to partial prevention of cold inactivation, mutant exists in dimeric and tetrameric form at low temperature Mus musculus
1.1.1.10 L242W/T244C site-directed mutagenesis, double mutation leads to partial prevention of cold inactivation, mutant exists in dimeric and tetrameric form at low temperature Mus musculus
1.1.1.10 T244C site-directed mutagenesis, mutant exists in dimeric form at low temperature like the wild-type enzyme resulting in cold inactivation Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.10 Homo sapiens
-
-
-
1.1.1.10 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.10 recombinant wild-type and mutant enzymes from Escherichia coli Mus musculus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.10 xylitol + NADP+ = L-xylulose + NADPH + H+ enzyme with dual function showing L-xylulose reductase and dicarbonyl reductase activities, it is probably identical with sperm 34 kDa protein P34H and diacetyl reductase, EC 1.1.1.5 Mus musculus
a
1.1.1.10 xylitol + NADP+ = L-xylulose + NADPH + H+ enzyme with dual function showing L-xylulose reductase and dicarbonyl reductase activities, it is probably identical with sperm 34 kDa protein P34H and diacetyl reductase, EC 1.1.1.5 Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.10 diacetyl + NAD(P)H dicarbonyl reductase activity Mus musculus acetoin + NAD(P)+
-
 r
1.1.1.10 diacetyl + NAD(P)H dicarbonyl reductase activity Homo sapiens acetoin + NAD(P)+
-
 r
1.1.1.10 L-xylulose + NADPH + H+
-
 Mus musculus L-xylitol + NADP+
-
 r
1.1.1.10 L-xylulose + NADPH + H+
-
 Homo sapiens L-xylitol + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.10 More the dimeric form is inactive Mus musculus
1.1.1.10 tetramer amino acid residues Trp242, Glu238, Arg203, and Cys244 are important for tetramer formation Mus musculus
1.1.1.10 tetramer amino acid residues Trp242, Glu238, Arg203, and Cys244 are important for tetramer formation Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.10 More enzyme belongs to the short-chain dehydrogenase/reductase family Mus musculus
1.1.1.10 More enzyme belongs to the short-chain dehydrogenase/reductase family Homo sapiens
1.1.1.10 XR
-
 Mus musculus
1.1.1.10 XR
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.10 25
-
 assay at Mus musculus
1.1.1.10 25
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.10 additional information
-
 dissociation into inactive dimers at low temperatures Mus musculus
1.1.1.10 additional information
-
 stable at low temperatures Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.10 7
-
 assay at Mus musculus
1.1.1.10 7
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.10 NADP+
-
 Mus musculus
1.1.1.10 NADP+
-
 Homo sapiens
1.1.1.10 NADPH
-
 Mus musculus
1.1.1.10 NADPH
-
 Homo sapiens