BRENDA - Enzyme Database

Selectivity studies of the benzene cis-dihydrodiol dehydrogenase enzyme from Pseudomonas putida ML2 with Vicinal diol substrates

Allen, C.C.R.; Walker, C.E.; Sharma, N.D.; Kerley, N.A.; Boyd, D.R.; Dalton, H.; Biocatal. Biotransform. 20, 257-264 (2002)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.1.19
0.068
-
NAD+
30°C, pH 8.0
Pseudomonas putida
1.3.1.19
0.12
-
cis-1,2-dihydrobenzene-1,2-diol
30°C, pH 8.0
Pseudomonas putida
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.1.19
40000
-
6 * 40000, SDS-PAGE
Pseudomonas putida
1.3.1.19
220000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.3.1.19
cis-1,2-dihydrobenzene-1,2-diol + NAD+
Pseudomonas putida
-
catechol + NADH
-
-
?
1.3.1.19
cis-1,2-dihydrobenzene-1,2-diol + NAD+
Pseudomonas putida ML2
-
catechol + NADH
-
-
?
1.3.1.19
cis-1,2-dihydrobenzene-1,2-diol + NAD+
Pseudomonas putida 8859
-
catechol + NADH
-
-
?
1.3.1.29
cis-1,2-dihydronaphthalene-1,2-diol + NAD+
Pseudomonas putida
-
naphthalene-1,2-diol + NADH
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.3.1.19
Pseudomonas putida
-
ML2
-
1.3.1.19
Pseudomonas putida ML2
-
ML2
-
1.3.1.29
Pseudomonas putida
-
-
-
1.3.1.29
Pseudomonas putida 8859
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.3.1.19
Q-Sepharose, Superdex 200, Mono Q
Pseudomonas putida
1.3.1.29
to homogeneity by ion exchange chromatography and gel filtration
Pseudomonas putida
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.1.29
culture condition:naphthalene-grown cell
-
Pseudomonas putida
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.1.19
27.5
-
-
Pseudomonas putida
1.3.1.29
12
-
purified enzyme, substrate (1R,2S)-cis-1,2-dihydronaphthalene-1,2-diol
Pseudomonas putida
1.3.1.29
16
-
purified enzyme, substrate (1S,2R)-1,2-dihydroxy-3-phenylcyclohexa-3,5-diene
Pseudomonas putida
1.3.1.29
19
-
purified enzyme, substrate (1S,2S)-1,2-dihydroxy-3-chlorocyclohexa-3,5-diene
Pseudomonas putida
1.3.1.29
20
-
purified enzyme, substrate (1S,2S)-1,2-dihydroxy-3-fluorocyclohexa-3,5-diene
Pseudomonas putida
1.3.1.29
20.6
-
purified enzyme, substrate (1S,2S)-1,2-dihydroxy-3-bromocyclohexa-3,5-diene
Pseudomonas putida
1.3.1.29
21.4
-
purified enzyme, substrate (1S,2S)-1,2-dihydroxy-3-iodocyclohexa-3,5-diene
Pseudomonas putida
1.3.1.29
22
-
purified enzyme, substrate (1S,2R)-1,2-dihydroxy-3-methylcyclohexa-3,5-diene
Pseudomonas putida
1.3.1.29
26
-
purified enzyme, substrate cis-1,2-dihydrobenzene-1,2-diol
Pseudomonas putida
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.3.1.19
5-methyl-hex-4-ene-1,2-diol + NAD+
25% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
5-methyl-hex-1,4-diene-1,2-diol + NADH + H+
-
-
-
?
1.3.1.19
5-methyl-hex-4-ene-1,2-diol + NAD+
25% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida ML2
5-methyl-hex-1,4-diene-1,2-diol + NADH + H+
-
-
-
?
1.3.1.19
5-methyl-hex-4-ene-1,2-diol + NAD+
25% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida 8859
5-methyl-hex-1,4-diene-1,2-diol + NADH + H+
-
-
-
?
1.3.1.19
7-methyl-oct-6-ene-1,2-diol + NAD+
13% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
7-methyl-oct-1,6-diene-1,2-diol + NADH + H+
-
-
-
?
1.3.1.19
7-methyl-oct-6-ene-1,2-diol + NAD+
13% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida ML2
7-methyl-oct-1,6-diene-1,2-diol + NADH + H+
-
-
-
?
1.3.1.19
7-methyl-oct-6-ene-1,2-diol + NAD+
13% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida 8859
7-methyl-oct-1,6-diene-1,2-diol + NADH + H+
-
-
-
?
1.3.1.19
butane-1,2-diol + NAD+
29% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
but-1-ene-1,2-diol + NADH
-
-
-
?
1.3.1.19
butane-1,2-diol + NAD+
29% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida ML2
but-1-ene-1,2-diol + NADH
-
-
-
?
1.3.1.19
butane-1,2-diol + NAD+
29% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida 8859
but-1-ene-1,2-diol + NADH
-
-
-
?
1.3.1.19
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
654397
Pseudomonas putida
catechol + NADH
-
-
-
?
1.3.1.19
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
654397
Pseudomonas putida ML2
catechol + NADH
-
-
-
?
1.3.1.19
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
654397
Pseudomonas putida 8859
catechol + NADH
-
-
-
?
1.3.1.19
cis-1,2-dihydrobenzene-3-bromo-(R,R)-1,2-diol + NAD+
7% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
3-bromocatechol + NADH
-
-
-
?
1.3.1.19
cis-1,2-dihydrobenzene-3-bromo-(R,R)-1,2-diol + NAD+
7% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida ML2
3-bromocatechol + NADH
-
-
-
?
1.3.1.19
cis-1,2-dihydrobenzene-3-bromo-(R,R)-1,2-diol + NAD+
7% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida 8859
3-bromocatechol + NADH
-
-
-
?
1.3.1.19
cis-1,2-dihydrobenzene-3-chloro-(R,R)-1,2-diol + NAD+
22% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
3-chlorocatechol + NADH
-
-
-
?
1.3.1.19
cis-1,2-dihydrobenzene-3-fluoro-(R,R)-1,2-diol + NAD+
35% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
3-fluorocatechol + NADH
-
-
-
?
1.3.1.19
decane-1,2-diol + NAD+
8% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
dec-1-ene-1,2-diol + NADH
-
-
-
?
1.3.1.19
nonane-1,2-diol + NAD+
15% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
non-1-ene-1,2-diol + NADH
-
-
-
?
1.3.1.19
pentane-1,2-diol + NAD+
28% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
pent-1-ene-1,2-diol + NADH
-
-
-
?
1.3.1.19
propane-1,2-diol + NAD+
36% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
prop-1-ene-1,2-diol + NADH
-
-
-
?
1.3.1.19
trimethylbutane-1,2-diol + NAD+
21% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
trimethylbut-1-ene-1,2-diol + NADH
-
-
-
?
1.3.1.29
(1R,2S)-cis-1,2-dihydronaphthalene-1,2-diol + NAD+
best substrate, stereospecific for the (1R,2S)-enantiomer, no activity with the (1S,2R)-enantiomer
654397
Pseudomonas putida
naphthalene-1,2-diol + NADH
-
-
-
?
1.3.1.29
(1S,2R)-1,2-dihydroxy-3-methylcyclohexa-3,5-diene + NAD+
-
654397
Pseudomonas putida
?
-
-
-
?
1.3.1.29
(1S,2R)-1,2-dihydroxy-3-phenylcyclohexa-3,5-diene + NAD+
-
654397
Pseudomonas putida
?
-
-
-
?
1.3.1.29
(1S,2S)-1,2-dihydroxy-3-bromocyclohexa-3,5-diene + NAD+
stereospecific for the (1S,2S)-enantiomer, no activity with the (1R,2R)-enantiomer
654397
Pseudomonas putida
?
-
-
-
?
1.3.1.29
(1S,2S)-1,2-dihydroxy-3-chlorocyclohexa-3,5-diene + NAD+
stereospecific for the (1S,2S)-enantiomer, no activity with the (1R,2R)-enantiomer
654397
Pseudomonas putida
?
-
-
-
?
1.3.1.29
(1S,2S)-1,2-dihydroxy-3-fluorocyclohexa-3,5-diene + NAD+
stereospecific for the (1S,2S)-enantiomer, no activity with the (1R,2R)-enantiomer
654397
Pseudomonas putida
?
-
-
-
?
1.3.1.29
(1S,2S)-1,2-dihydroxy-3-iodocyclohexa-3,5-diene + NAD+
stereospecific for the (1S,2S)-enantiomer, no activity with the (1R,2R)-enantiomer
654397
Pseudomonas putida
?
-
-
-
?
1.3.1.29
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
654397
Pseudomonas putida
benzene-1,2-diol + NADH
-
-
-
?
1.3.1.29
cis-1,2-dihydronaphthalene-1,2-diol + NAD+
-
654397
Pseudomonas putida
naphthalene-1,2-diol + NADH
-
-
-
?
1.3.1.29
additional information
substrate specificity and stereoselectivity, no oxidation of any vicinal alkene diol substrate
654397
Pseudomonas putida
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.3.1.19
hexamer
6 * 40000, SDS-PAGE
Pseudomonas putida
Synonyms
EC Number
Synonyms
Commentary
Organism
1.3.1.29
naphthalene cis-dihydrodiol dehydrogenase
-
Pseudomonas putida
1.3.1.29
ncd
-
Pseudomonas putida
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.3.1.29
30
-
assay at
Pseudomonas putida
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.1.29
8
-
assay at
Pseudomonas putida
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.1.19
NAD+
-
Pseudomonas putida
1.3.1.29
NAD+
-
Pseudomonas putida
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.1.19
NAD+
-
Pseudomonas putida
1.3.1.29
NAD+
-
Pseudomonas putida
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.1.19
0.068
-
NAD+
30°C, pH 8.0
Pseudomonas putida
1.3.1.19
0.12
-
cis-1,2-dihydrobenzene-1,2-diol
30°C, pH 8.0
Pseudomonas putida
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.1.19
40000
-
6 * 40000, SDS-PAGE
Pseudomonas putida
1.3.1.19
220000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.3.1.19
cis-1,2-dihydrobenzene-1,2-diol + NAD+
Pseudomonas putida
-
catechol + NADH
-
-
?
1.3.1.19
cis-1,2-dihydrobenzene-1,2-diol + NAD+
Pseudomonas putida ML2
-
catechol + NADH
-
-
?
1.3.1.19
cis-1,2-dihydrobenzene-1,2-diol + NAD+
Pseudomonas putida 8859
-
catechol + NADH
-
-
?
1.3.1.29
cis-1,2-dihydronaphthalene-1,2-diol + NAD+
Pseudomonas putida
-
naphthalene-1,2-diol + NADH
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.1.19
Q-Sepharose, Superdex 200, Mono Q
Pseudomonas putida
1.3.1.29
to homogeneity by ion exchange chromatography and gel filtration
Pseudomonas putida
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.1.29
culture condition:naphthalene-grown cell
-
Pseudomonas putida
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.1.19
27.5
-
-
Pseudomonas putida
1.3.1.29
12
-
purified enzyme, substrate (1R,2S)-cis-1,2-dihydronaphthalene-1,2-diol
Pseudomonas putida
1.3.1.29
16
-
purified enzyme, substrate (1S,2R)-1,2-dihydroxy-3-phenylcyclohexa-3,5-diene
Pseudomonas putida
1.3.1.29
19
-
purified enzyme, substrate (1S,2S)-1,2-dihydroxy-3-chlorocyclohexa-3,5-diene
Pseudomonas putida
1.3.1.29
20
-
purified enzyme, substrate (1S,2S)-1,2-dihydroxy-3-fluorocyclohexa-3,5-diene
Pseudomonas putida
1.3.1.29
20.6
-
purified enzyme, substrate (1S,2S)-1,2-dihydroxy-3-bromocyclohexa-3,5-diene
Pseudomonas putida
1.3.1.29
21.4
-
purified enzyme, substrate (1S,2S)-1,2-dihydroxy-3-iodocyclohexa-3,5-diene
Pseudomonas putida
1.3.1.29
22
-
purified enzyme, substrate (1S,2R)-1,2-dihydroxy-3-methylcyclohexa-3,5-diene
Pseudomonas putida
1.3.1.29
26
-
purified enzyme, substrate cis-1,2-dihydrobenzene-1,2-diol
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.3.1.19
5-methyl-hex-4-ene-1,2-diol + NAD+
25% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
5-methyl-hex-1,4-diene-1,2-diol + NADH + H+
-
-
-
?
1.3.1.19
5-methyl-hex-4-ene-1,2-diol + NAD+
25% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida ML2
5-methyl-hex-1,4-diene-1,2-diol + NADH + H+
-
-
-
?
1.3.1.19
5-methyl-hex-4-ene-1,2-diol + NAD+
25% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida 8859
5-methyl-hex-1,4-diene-1,2-diol + NADH + H+
-
-
-
?
1.3.1.19
7-methyl-oct-6-ene-1,2-diol + NAD+
13% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
7-methyl-oct-1,6-diene-1,2-diol + NADH + H+
-
-
-
?
1.3.1.19
7-methyl-oct-6-ene-1,2-diol + NAD+
13% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida ML2
7-methyl-oct-1,6-diene-1,2-diol + NADH + H+
-
-
-
?
1.3.1.19
7-methyl-oct-6-ene-1,2-diol + NAD+
13% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida 8859
7-methyl-oct-1,6-diene-1,2-diol + NADH + H+
-
-
-
?
1.3.1.19
butane-1,2-diol + NAD+
29% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
but-1-ene-1,2-diol + NADH
-
-
-
?
1.3.1.19
butane-1,2-diol + NAD+
29% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida ML2
but-1-ene-1,2-diol + NADH
-
-
-
?
1.3.1.19
butane-1,2-diol + NAD+
29% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida 8859
but-1-ene-1,2-diol + NADH
-
-
-
?
1.3.1.19
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
654397
Pseudomonas putida
catechol + NADH
-
-
-
?
1.3.1.19
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
654397
Pseudomonas putida ML2
catechol + NADH
-
-
-
?
1.3.1.19
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
654397
Pseudomonas putida 8859
catechol + NADH
-
-
-
?
1.3.1.19
cis-1,2-dihydrobenzene-3-bromo-(R,R)-1,2-diol + NAD+
7% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
3-bromocatechol + NADH
-
-
-
?
1.3.1.19
cis-1,2-dihydrobenzene-3-bromo-(R,R)-1,2-diol + NAD+
7% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida ML2
3-bromocatechol + NADH
-
-
-
?
1.3.1.19
cis-1,2-dihydrobenzene-3-bromo-(R,R)-1,2-diol + NAD+
7% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida 8859
3-bromocatechol + NADH
-
-
-
?
1.3.1.19
cis-1,2-dihydrobenzene-3-chloro-(R,R)-1,2-diol + NAD+
22% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
3-chlorocatechol + NADH
-
-
-
?
1.3.1.19
cis-1,2-dihydrobenzene-3-fluoro-(R,R)-1,2-diol + NAD+
35% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
3-fluorocatechol + NADH
-
-
-
?
1.3.1.19
decane-1,2-diol + NAD+
8% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
dec-1-ene-1,2-diol + NADH
-
-
-
?
1.3.1.19
nonane-1,2-diol + NAD+
15% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
non-1-ene-1,2-diol + NADH
-
-
-
?
1.3.1.19
pentane-1,2-diol + NAD+
28% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
pent-1-ene-1,2-diol + NADH
-
-
-
?
1.3.1.19
propane-1,2-diol + NAD+
36% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
prop-1-ene-1,2-diol + NADH
-
-
-
?
1.3.1.19
trimethylbutane-1,2-diol + NAD+
21% of activity with cis-1,2-dihydrobenzene-1,2-diol
654397
Pseudomonas putida
trimethylbut-1-ene-1,2-diol + NADH
-
-
-
?
1.3.1.29
(1R,2S)-cis-1,2-dihydronaphthalene-1,2-diol + NAD+
best substrate, stereospecific for the (1R,2S)-enantiomer, no activity with the (1S,2R)-enantiomer
654397
Pseudomonas putida
naphthalene-1,2-diol + NADH
-
-
-
?
1.3.1.29
(1S,2R)-1,2-dihydroxy-3-methylcyclohexa-3,5-diene + NAD+
-
654397
Pseudomonas putida
?
-
-
-
?
1.3.1.29
(1S,2R)-1,2-dihydroxy-3-phenylcyclohexa-3,5-diene + NAD+
-
654397
Pseudomonas putida
?
-
-
-
?
1.3.1.29
(1S,2S)-1,2-dihydroxy-3-bromocyclohexa-3,5-diene + NAD+
stereospecific for the (1S,2S)-enantiomer, no activity with the (1R,2R)-enantiomer
654397
Pseudomonas putida
?
-
-
-
?
1.3.1.29
(1S,2S)-1,2-dihydroxy-3-chlorocyclohexa-3,5-diene + NAD+
stereospecific for the (1S,2S)-enantiomer, no activity with the (1R,2R)-enantiomer
654397
Pseudomonas putida
?
-
-
-
?
1.3.1.29
(1S,2S)-1,2-dihydroxy-3-fluorocyclohexa-3,5-diene + NAD+
stereospecific for the (1S,2S)-enantiomer, no activity with the (1R,2R)-enantiomer
654397
Pseudomonas putida
?
-
-
-
?
1.3.1.29
(1S,2S)-1,2-dihydroxy-3-iodocyclohexa-3,5-diene + NAD+
stereospecific for the (1S,2S)-enantiomer, no activity with the (1R,2R)-enantiomer
654397
Pseudomonas putida
?
-
-
-
?
1.3.1.29
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
654397
Pseudomonas putida
benzene-1,2-diol + NADH
-
-
-
?
1.3.1.29
cis-1,2-dihydronaphthalene-1,2-diol + NAD+
-
654397
Pseudomonas putida
naphthalene-1,2-diol + NADH
-
-
-
?
1.3.1.29
additional information
substrate specificity and stereoselectivity, no oxidation of any vicinal alkene diol substrate
654397
Pseudomonas putida
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.3.1.19
hexamer
6 * 40000, SDS-PAGE
Pseudomonas putida
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.3.1.29
30
-
assay at
Pseudomonas putida
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.1.29
8
-
assay at
Pseudomonas putida