EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.67 | expression in Escherichia coli | Leuconostoc pseudomesenteroides |
EC Number | General Stability | Organism |
---|---|---|
1.1.1.67 | DTT stabilizes | Leuconostoc pseudomesenteroides |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.67 | citrate | 50 mM, pH 5.4, 72% inhibition | Leuconostoc pseudomesenteroides | |
1.1.1.67 | EDTA | 20 mM, complete inhibition, can be restored by 20 mM Zn2+, but not by Mn2+ or Mg2+ | Leuconostoc pseudomesenteroides | |
1.1.1.67 | imidazole | 175 mM,pH 7.0, 74% inhibition | Leuconostoc pseudomesenteroides |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.67 | 12 | - |
D-mannitol | pH 8.6, 30°C | Leuconostoc pseudomesenteroides | |
1.1.1.67 | 44 | - |
D-fructose | pH 5.4, 30°C | Leuconostoc pseudomesenteroides |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.67 | Zn2+ | cofactor | Leuconostoc pseudomesenteroides |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.67 | 43000 | - |
4 * 43000, SDS-PAGE | Leuconostoc pseudomesenteroides |
1.1.1.67 | 155000 | - |
gel filtration | Leuconostoc pseudomesenteroides |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.67 | Leuconostoc pseudomesenteroides | Q83VI5 | ATCC 12291 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.67 | - |
Leuconostoc pseudomesenteroides |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.67 | 450 | - |
- |
Leuconostoc pseudomesenteroides |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.67 | 4°C or 20°C, enzyme loses approximately 60% of its specific activity after storage for 3 weeks in presence of 1 mM DTT, without DTT the same loss is observed after 2 days | Leuconostoc pseudomesenteroides |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.67 | D-fructose + NADH + H+ | - |
Leuconostoc pseudomesenteroides | D-mannitol + NAD+ | - |
r | |
1.1.1.67 | D-glucitol + NAD+ | 4% of the activity with D-mannitol | Leuconostoc pseudomesenteroides | ? | - |
? | |
1.1.1.67 | D-mannitol + NADP+ | 32% of the activity with NAD+ | Leuconostoc pseudomesenteroides | D-fructose + NADPH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.67 | tetramer | 4 * 43000, SDS-PAGE | Leuconostoc pseudomesenteroides |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.67 | mannitol 2-dehydrogenase | - |
Leuconostoc pseudomesenteroides |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.67 | 5.4 | - |
reduction of D-fructose | Leuconostoc pseudomesenteroides |
1.1.1.67 | 8.6 | - |
oxidation of D-mannitol | Leuconostoc pseudomesenteroides |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.67 | NAD+ | - |
Leuconostoc pseudomesenteroides | |
1.1.1.67 | NADH | - |
Leuconostoc pseudomesenteroides | |
1.1.1.67 | NADP+ | 32% of the activity with NAD+ | Leuconostoc pseudomesenteroides |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.1.1.67 | Leuconostoc pseudomesenteroides | chromatofocusing | - |
4.2 |