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Literature summary extracted from

  • Cho, H.; Hamza, A.; Zhan, C.G.; Tai, H.H.
    Key NAD+-binding residues in human 15-hydroxyprostaglandin dehydrogenase (2005), Arch. Biochem. Biophys., 433, 447-453.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.141 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.141 I17A 270% of wild-type activity Homo sapiens
1.1.1.141 I17E no activity Homo sapiens
1.1.1.141 I17K no activity Homo sapiens
1.1.1.141 I17L 364% of wild-type activity Homo sapiens
1.1.1.141 I17V 300% of wild-type activity Homo sapiens
1.1.1.141 N91A 136% of wild-type activity Homo sapiens
1.1.1.141 N91D 86% of wild-type activity Homo sapiens
1.1.1.141 N91K no activity Homo sapiens
1.1.1.141 N91L no activity Homo sapiens
1.1.1.141 V186A 143% of wild-type activity Homo sapiens
1.1.1.141 V186D no activity Homo sapiens
1.1.1.141 V186I 468% of wild-type activity Homo sapiens
1.1.1.141 V186K 71% of wild-type activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.141 0.0012
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5, I17L mutant Homo sapiens
1.1.1.141 0.0026
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5, N91D mutant Homo sapiens
1.1.1.141 0.0034
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5 Homo sapiens
1.1.1.141 0.0035
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5, V186K mutant Homo sapiens
1.1.1.141 0.0045
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5, V186A mutant Homo sapiens
1.1.1.141 0.006
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5, N91A mutant Homo sapiens
1.1.1.141 0.011
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5, I17A mutant Homo sapiens
1.1.1.141 0.011
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5, I17V mutant Homo sapiens
1.1.1.141 0.022
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5, V186I mutant Homo sapiens
1.1.1.141 0.022
-
 NAD+ 37°C, pH 7.5, V186K mutant Homo sapiens
1.1.1.141 0.024
-
 NAD+ 37°C, pH 7.5, V186A mutant Homo sapiens
1.1.1.141 0.032
-
 NAD+ 37°C, pH 7.5, N91D mutant Homo sapiens
1.1.1.141 0.038
-
 NAD+ 37°C, pH 7.5 Homo sapiens
1.1.1.141 0.059
-
 NAD+ 37°C, pH 7.5, I17L mutant Homo sapiens
1.1.1.141 0.098
-
 NAD+ 37°C, pH 7.5, I17V mutant Homo sapiens
1.1.1.141 0.27
-
 NAD+ 37°C, pH 7.5, V186I mutant Homo sapiens
1.1.1.141 0.55
-
 NAD+ 37°C, pH 7.5, I17A mutant Homo sapiens
1.1.1.141 0.72
-
 NAD+ 37°C, pH 7.5, N91A mutant Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.141 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ Homo sapiens
-
 (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.141 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.141 recombinant 15-PGDH Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.141 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+
-
 Homo sapiens (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.141 15-PGDH
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.141 35
-
 NAD+ 37°C, pH 7.5, V186K mutant Homo sapiens
1.1.1.141 37
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5, V186K mutant Homo sapiens
1.1.1.141 42
-
 NAD+ 37°C, pH 7.5, N91D mutant Homo sapiens
1.1.1.141 45
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5, N91D mutant Homo sapiens
1.1.1.141 47
-
 NAD+ 37°C, pH 7.5 Homo sapiens
1.1.1.141 50
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5 Homo sapiens
1.1.1.141 70
-
 NAD+ 37°C, pH 7.5, V186A mutant Homo sapiens
1.1.1.141 73
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5, N91A mutant Homo sapiens
1.1.1.141 78
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5, V186A mutant Homo sapiens
1.1.1.141 91
-
 NAD+ 37°C, pH 7.5, N91A mutant Homo sapiens
1.1.1.141 110
-
 NAD+ 37°C, pH 7.5, I17V mutant Homo sapiens
1.1.1.141 127
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5, I17A mutant Homo sapiens
1.1.1.141 129
-
 NAD+ 37°C, pH 7.5, I17A mutant Homo sapiens
1.1.1.141 143
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5, I17V mutant Homo sapiens
1.1.1.141 165
-
 NAD+ 37°C, pH 7.5, I17L mutant Homo sapiens
1.1.1.141 210
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5, I17L mutant Homo sapiens
1.1.1.141 247
-
 NAD+ 37°C, pH 7.5, V186I mutant Homo sapiens
1.1.1.141 262
-
 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate 37°C, pH 7.5, V186I mutant Homo sapiens