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Literature summary extracted from

  • Thelen, J.J.; Ohlrogge, J.B.
    The multisubunit acetyl-CoA carboxylase is strongly associated with the chloroplast envelope through non-ionic interactions to the carboxyltransferase subunits (2002), Arch. Biochem. Biophys., 400, 245-257.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.4.14 chloroplast BC is present in the major plastid compartments envelopes, stroma and thylakoids, but predominantly localized in the stroma, presumably due to partial dissociation as a result of the isolation procedure Pisum sativum 9507
-
6.3.4.14 membrane might be associated with the membrane, BC of the undissociated complex acetyl-CoA carboxylase Pisum sativum 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.14 additional information Pisum sativum pathway from acetyl-CoA to long-chain fatty acids, developmental regulation of enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.14 Pisum sativum
-
 cv. Little Marvel
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.14 partial Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.14 leaf developing, 7 days old Pisum sativum
-
6.3.4.14 seed 7 days old Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.14 additional information
-
-
 Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.14 ATP + biotin-carboxyl-carrier protein + HCO3- BC component of the multisubunit complex acetyl-CoA carboxylase, BC catalyzes the ATP-dependent carboxylation of the biotinyl moiety on biotin carboxyl carrier protein Pisum sativum ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
 ?
6.3.4.14 ATP + d-biotin + HCO3- BC component of the multisubunit complex of acetyl-CoA carboxylase Pisum sativum ADP + phosphate + carboxy-d-biotin
-
 ?
6.3.4.14 additional information pathway from acetyl-CoA to long-chain fatty acids, developmental regulation of enzyme Pisum sativum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.4.14 25
-
 assay at Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.14 8
-
 assay at Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.14 ATP ATP-dependent Pisum sativum