Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Salusjarvi, T.; Povelainen, M.; Hvorslev, N.; Eneyskaya, E.V.; Kulminskaya, A.A.; Shabalin, K.A.; Neustroev, K.N.; Kalkkinen, N.; Miasnikov, A.N.
    Cloning of a gluconate/polyol dehydrogenase gene from Gluconobacter suboxydans IFO 12528, characterisation of the enzyme and its use for the production of 5-ketogluconate in a recombinant Escherichia coli strain (2004), Appl. Microbiol. Biotechnol., 65, 306-314.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.69 4.6
-
 D-arabitol pH 5.5, 30°C Gluconobacter oxydans
1.1.1.69 57
-
 D-sorbitol pH 5.5, 30°C Gluconobacter oxydans
1.1.1.69 161
-
 D-gluconic acid pH 5.5, 30°C Gluconobacter oxydans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.69 membrane
-
 Gluconobacter oxydans 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.69 80000
-
 x * 80000, SDS-PAGE Gluconobacter oxydans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.69 Gluconobacter oxydans Q70JN9 large subunit; recombinant enzyme
-
1.1.1.69 Gluconobacter oxydans Q70JP0 small subunit; recombinant enzyme
-
1.1.1.69 Gluconobacter oxydans IFO 12528 Q70JN9 large subunit; recombinant enzyme
-
1.1.1.69 Gluconobacter oxydans IFO 12528 Q70JP0 small subunit; recombinant enzyme
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.69 200
-
 pH 5.5, 30°C Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.69 2,3-butanediol + NAD+ 169% of activity with D-sorbitol Gluconobacter oxydans ? + NADH
-
 ?
1.1.1.69 2,3-butanediol + NAD+ 169% of activity with D-sorbitol Gluconobacter oxydans IFO 12528 ? + NADH
-
 ?
1.1.1.69 D-arabitol + NAD+ 130% of activity with D-sorbitol Gluconobacter oxydans ? + NADH
-
 ?
1.1.1.69 D-arabitol + NAD+ 130% of activity with D-sorbitol Gluconobacter oxydans IFO 12528 ? + NADH
-
 ?
1.1.1.69 D-gluconic acid + NAD+ 8% of activity with D-sorbitol Gluconobacter oxydans ? + NADH
-
 ?
1.1.1.69 D-gluconic acid + NAD+ 8% of activity with D-sorbitol Gluconobacter oxydans IFO 12528 ? + NADH
-
 ?
1.1.1.69 D-mannitol + NAD+ 74% of activity with D-sorbitol Gluconobacter oxydans ? + NADH
-
 ?
1.1.1.69 D-mannitol + NAD+ 74% of activity with D-sorbitol Gluconobacter oxydans IFO 12528 ? + NADH
-
 ?
1.1.1.69 D-sorbitol + NAD+
-
 Gluconobacter oxydans ? + NADH
-
 ?
1.1.1.69 D-sorbitol + NAD+
-
 Gluconobacter oxydans IFO 12528 ? + NADH
-
 ?
1.1.1.69 glycerol + NAD+ 138% of activity with D-sorbitol Gluconobacter oxydans ? + NADH
-
 ?
1.1.1.69 meso-erytritol + NAD+ 143% of activity with D-sorbitol Gluconobacter oxydans ? + NADH
-
 ?
1.1.1.69 ribitol + NAD+ 66% of activity with D-sorbitol Gluconobacter oxydans ? + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.69 ? x * 80000, SDS-PAGE Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.69 6
-
-
 Gluconobacter oxydans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.69 4
-
 fairly stable Gluconobacter oxydans
1.1.1.69 8
-
 unstable Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.69 NAD+
-
 Gluconobacter oxydans
1.1.1.69 NADH
-
 Gluconobacter oxydans