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Literature summary extracted from

  • Politz, O.; Krah, M.; Thomsen, K.K.; Borriss, R.
    A highly thermostable endo-(1,4)-beta-mannanase from the marine bacterium Rhodothermus marinus (2000), Appl. Microbiol. Biotechnol., 53, 715-721.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78
-
 Rhodothermus marinus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 113000
-
 x * 113000, deduced from gene sequence, upon expression in Escherichia coli, two fragments of 45000 and 50000 Da Rhodothermus marinus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Rhodothermus marinus P49425 ATCC 43812
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 carob-galactomannan + H2O i.e. locust bean gum Rhodothermus marinus ?
-
 ?
3.2.1.78 guar gum + H2O
-
 Rhodothermus marinus ?
-
 ?
3.2.1.78 additional information requires at least five sugar moieties for effective catalytic activity, no substrate: yeast mannan Rhodothermus marinus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 113000, deduced from gene sequence, upon expression in Escherichia coli, two fragments of 45000 and 50000 Da Rhodothermus marinus
3.2.1.78 More C-terminal domain of 550 amino acid residues with homology to glycosidase family 26 Rhodothermus marinus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 85
-
-
 Rhodothermus marinus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 70
-
 1 h, 70% residual activity, truncated protein fragments produced in Escherichia coli, 1 h, 87% residual activity, protein derived from Rhodothermus marinus Rhodothermus marinus
3.2.1.78 90
-
 1 h, 25% residual activity, truncated protein fragments produced in Escherichia coli Rhodothermus marinus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 5.4
-
-
 Rhodothermus marinus