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Literature summary extracted from

  • Damaso, M.C.T.; Almeida, M.S.; Kurtenbach, E.; Martins, O.B.; Pereira, N.Jr.; Andrade, C.M.M.C.; Albano, R.M.
    Optimized expression of a thermostable xylanase from Thermomyces lanuginosus in Pichia pastoris (2003), Appl. Environ. Microbiol., 69, 6064-6072.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 gene XynA, overexpression in Pichia pastoris, method optimization, functional expression under control of the AOX1 promotor Thermomyces lanuginosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Thermomyces lanuginosus O43097 gene XynA
-
3.2.1.8 Thermomyces lanuginosus IOC-4145 O43097 gene XynA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 recombinant enzyme from Pichia pastoris, and native enzyme, by gel filtration Thermomyces lanuginosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 xylan + H2O
-
 Thermomyces lanuginosus ?
-
 ?
3.2.1.8 xylan + H2O
-
 Thermomyces lanuginosus IOC-4145 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 21900-22300, recombinant enzyme, SDS-PAGE Thermomyces lanuginosus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 xylanase
-
 Thermomyces lanuginosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 75
-
 native and recombinant enzyme Thermomyces lanuginosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 70
-
 80 min, 60% remaining activity, native and recombinant enzyme Thermomyces lanuginosus
3.2.1.8 80
-
 40 min, 60% remaining activity, native and recombinant enzyme Thermomyces lanuginosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6
-
 assay at Thermomyces lanuginosus