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Literature summary extracted from

  • Margolles, A.; de los Reyes-Gavillan, C.G.
    Purification and functional characterization of a novel alpha-L-arabinofuranosidase from Bifidobacterium longum B667 (2003), Appl. Environ. Microbiol., 69, 5096-5103.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 DNA and amino acid sequence determination and analysis, overexpression of the C-terminally His-tagged enzyme in Lactococcus lactis NZ9000 under control of nisin-inducible nisA promotor Bifidobacterium longum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.55 Cu2+
-
 Bifidobacterium longum
3.2.1.55 Hg2+
-
 Bifidobacterium longum
3.2.1.55 additional information not affected by chelating or reducing agents Bifidobacterium longum
3.2.1.55 Zn2+
-
 Bifidobacterium longum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.295
-
 4-nitrophenyl-alpha-L-arabinofuranoside pH 6.0, 45°C Bifidobacterium longum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 64000
-
 4 * 64000, SDS-PAGE Bifidobacterium longum
3.2.1.55 260000
-
 detagged enzyme, gel filtration Bifidobacterium longum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.55 additional information Bifidobacterium longum enzyme might have a role in the degradation of L-arabinose-containing polysaccharides together with other glycosidases ?
-
 ?
3.2.1.55 additional information Bifidobacterium longum B667 enzyme might have a role in the degradation of L-arabinose-containing polysaccharides together with other glycosidases ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Bifidobacterium longum Q841V6
-
-
3.2.1.55 Bifidobacterium longum B667 Q841V6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55 recombinant His-tagged enzyme from Lactococcus lactis by Ni2+-chelate affinity chromatography Bifidobacterium longum

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.55 1,5-alpha-L-arabinofuranohexaose + 5 H2O = 6 alpha-L-arabinofuranose exo-acting enzyme Bifidobacterium longum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Bifidobacterium longum 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Bifidobacterium longum B667 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 alpha-(1->5)-L-arabinofuranotriose + H2O
-
 Bifidobacterium longum alpha-L-arabinofuranose
-
 ?
3.2.1.55 alpha-L-arabinofuranobiose + H2O
-
 Bifidobacterium longum alpha-L-arabinofuranose
-
 ?
3.2.1.55 alpha-L-arabinofuranobiose + H2O
-
 Bifidobacterium longum B667 alpha-L-arabinofuranose
-
 ?
3.2.1.55 alpha-L-arabinofuranopentaose + H2O
-
 Bifidobacterium longum alpha-L-arabinofuranose
-
 ?
3.2.1.55 alpha-L-arabinofuranopentaose + H2O
-
 Bifidobacterium longum B667 alpha-L-arabinofuranose
-
 ?
3.2.1.55 alpha-L-arabinofuranoptetraose + H2O
-
 Bifidobacterium longum alpha-L-arabinofuranose
-
 ?
3.2.1.55 arabinan + H2O
-
 Bifidobacterium longum alpha-L-arabinose + ?
-
 ?
3.2.1.55 arabinoxylan + H2O
-
 Bifidobacterium longum alpha-L-arabinose + ?
-
 ?
3.2.1.55 additional information enzyme might have a role in the degradation of L-arabinose-containing polysaccharides together with other glycosidases Bifidobacterium longum ?
-
 ?
3.2.1.55 additional information substrate specificity, no endoarabinanase activity Bifidobacterium longum ?
-
 ?
3.2.1.55 additional information enzyme might have a role in the degradation of L-arabinose-containing polysaccharides together with other glycosidases Bifidobacterium longum B667 ?
-
 ?
3.2.1.55 additional information substrate specificity, no endoarabinanase activity Bifidobacterium longum B667 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 tetramer 4 * 64000, SDS-PAGE Bifidobacterium longum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 AbfB
-
 Bifidobacterium longum
3.2.1.55 alpha-L-arabinofuranosidase
-
 Bifidobacterium longum
3.2.1.55 More enzyme belongs to the family 51 of glycosidases Bifidobacterium longum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 45
-
-
 Bifidobacterium longum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.55 20 70
-
 Bifidobacterium longum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 55
-
 half-life: 3 h Bifidobacterium longum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 6
-
-
 Bifidobacterium longum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.55 4.5 7.5
-
 Bifidobacterium longum