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Literature summary extracted from
- The murein hydrolase of the bacteriophage phi3626 dual lysis system is active against all tested Clostridium perfringens strains (2002), Appl. Environ. Microbiol., 68, 5311-5317.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.17 | nutrition | Ply3626, which is highly specific towards Clostridium perfringens strains, might be useful for novel biocontrol measures in food, feed, and complex microbial communities, application as a novel biopreservative in food items such as raw chicken or turkey | Clostridium phage phi3626 |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.17 | ply3626 gene, expression in Escherichia coli JM109 | Clostridium phage phi3626 |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.17 | 38800 | - |
x * 38800, SDS-PAGE | Clostridium phage phi3626 |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.17 | Clostridium phage phi3626 | - |
murein hydrolase, endolysin Ply3626 of dual lysis system consisting of holin and endolysin | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.17 | recombinant Ply3626 | Clostridium phage phi3626 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.17 | peptidoglycan + H2O | murein hydrolase, highly specific towards cell walls of Clostridium perfringens strains, endolysin Ply3626 has an N-terminal N-acetylmuramoyl-L-alanine amidase domain and a unique C-terminal portion, which might be responsible for the specific lytic range of enzyme | Clostridium phage phi3626 | ? | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.17 | ? | x * 38800, SDS-PAGE | Clostridium phage phi3626 |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.17 | Ply3626 | endolysin of bacteriophage phi3626 | Clostridium phage phi3626 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.17 | 37 | - |
assay at | Clostridium phage phi3626 |
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