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Literature summary extracted from

  • Takao, M.; Akiyama, K.; Sakai, T.
    Purification and characterization of thermostable endo-1,5-alpha-L-arabinase from a strain of Bacillus thermodenitrificans (2002), Appl. Environ. Microbiol., 68, 1639-1646.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.99 Ag2+ complete inhibition at 1 mM Geobacillus thermodenitrificans
3.2.1.99 Hg2+ complete inhibition at 1 mM Geobacillus thermodenitrificans
3.2.1.99 additional information no inhibition by Ba2+, Mn2+ and Mg2+ Geobacillus thermodenitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.99 additional information
-
 additional information
-
 Geobacillus thermodenitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.99 35000
-
 SDS-PAGE Geobacillus thermodenitrificans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.99 Geobacillus thermodenitrificans AB063312
-
-
3.2.1.99 Geobacillus thermodenitrificans TS-3 AB063312
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.99 7400fold Geobacillus thermodenitrificans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.99 445
-
-
 Geobacillus thermodenitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.99 1,5-alpha-L-arabinan + H2O i.e. debranched arabinan Geobacillus thermodenitrificans alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
 ?
3.2.1.99 1,5-alpha-L-arabinan + H2O i.e. debranched arabinan Geobacillus thermodenitrificans TS-3 alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
 ?
3.2.1.99 arabinan + H2O
-
 Geobacillus thermodenitrificans ?
-
 ?
3.2.1.99 arabinan + H2O
-
 Geobacillus thermodenitrificans TS-3 ?
-
 ?
3.2.1.99 additional information no substrates: carboxymethyl cellulose, xylan, polygalacturonate, pectin, galactan, 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside,4-nitrophenyl-alpha-D-xylopyranoside and 4-nitrophenyl-beta-D-cellobioside Geobacillus thermodenitrificans ?
-
 ?
3.2.1.99 additional information no substrates: carboxymethyl cellulose, xylan, polygalacturonate, pectin, galactan, 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside,4-nitrophenyl-alpha-D-xylopyranoside and 4-nitrophenyl-beta-D-cellobioside Geobacillus thermodenitrificans TS-3 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.99 ABN
-
 Geobacillus thermodenitrificans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.99 70
-
-
 Geobacillus thermodenitrificans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.99 75
-
 half-life: 4 h Geobacillus thermodenitrificans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.99 6
-
-
 Geobacillus thermodenitrificans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.99 6 11 quite stable at 37°C for 16 h Geobacillus thermodenitrificans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.99 Geobacillus thermodenitrificans isoelectric focusing, pH gradient 3-10
-
 4.5