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Literature summary extracted from
- Cloning, expression, and characterization of thermostable region of amylopullulanase gene from Thermoanaerobacter ethanolicus 39E (2002), Appl. Biochem. Biotechnol., 97, 33-44.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.41 | - |
Thermoanaerobacter ethanolicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.41 | alpha-cyclodextrin | - |
Thermoanaerobacter ethanolicus |  |
| 3.2.1.41 | EDTA | - |
Thermoanaerobacter ethanolicus | |
| 3.2.1.41 | N-bromosuccinimide | - |
Thermoanaerobacter ethanolicus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.41 | Ca2+ | activation and stabilization | Thermoanaerobacter ethanolicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.41 | 109000 | - |
x * 109000, SDS-PAGE | Thermoanaerobacter ethanolicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.41 | Thermoanaerobacter ethanolicus | - |
39E, bifunctional enzyme with activities of both alpha-amylase and pullulanase | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.41 | recombinant protein expressed in Escherichia coli | Thermoanaerobacter ethanolicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.41 | amylopectin + H2O | - |
Thermoanaerobacter ethanolicus | malto-2-4-oligosaccharide + ? | - |
? | |
| 3.2.1.41 | amylose + H2O | - |
Thermoanaerobacter ethanolicus | malto-2-4-oligosaccharide + ? | - |
? | |
| 3.2.1.41 | glycogen + H2O | - |
Thermoanaerobacter ethanolicus | malto-2-4-oligosaccharide + ? | - |
? | |
| 3.2.1.41 | pullulan + H2O | best substrate | Thermoanaerobacter ethanolicus | malto-2-4-oligosaccharide + ? | - |
? | |
| 3.2.1.41 | soluble starch + H2O | - |
Thermoanaerobacter ethanolicus | malto-2-4-oligosaccharide + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.41 | ? | x * 109000, SDS-PAGE | Thermoanaerobacter ethanolicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.41 | 80 | - |
pullulanase activity | Thermoanaerobacter ethanolicus |
| 3.2.1.41 | 90 | - |
alpha-amylase activity | Thermoanaerobacter ethanolicus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.41 | 75 | - |
pullulanase activity | Thermoanaerobacter ethanolicus |
| 3.2.1.41 | 80 | - |
alpha-amylase activity | Thermoanaerobacter ethanolicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.41 | 6 | - |
- |
Thermoanaerobacter ethanolicus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.41 | 5 | 5.5 | - |
Thermoanaerobacter ethanolicus |
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