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Literature summary extracted from

  • Cunha Lima, S.T.; Azevedo, R.A.; Santoro, L.G.; Gaziola, S.A.; Lea, P.J.
    Isolation of the bifunctional enzyme lysine 2-oxoglutarate reductase-saccharopine dehydrogenase from Phaseolus vulgaris (2003), Amino Acids, 24, 179-186.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.1.8 lysine 2-oxoglutarate reductase activity is very unstable during purification, following ion-exchange chromatography Phaseolus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.8 190000
-
 bifunctional enzyme lysine 2-oxoglutarate reductase-saccharopine dehydrogenase, gel filtration Phaseolus vulgaris
1.5.1.9 190000
-
 bifunctional enzyme lysine 2-oxoglutarate reductase-saccharopine dehydrogenase, gel filtration Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.8 Phaseolus vulgaris
-
-
-
1.5.1.9 Phaseolus vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.8
-
 Phaseolus vulgaris
1.5.1.9
-
 Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.8 cotyledon
-
 Phaseolus vulgaris
-
1.5.1.8 hypocotyl
-
 Phaseolus vulgaris
-
1.5.1.8 leaf
-
 Phaseolus vulgaris
-
1.5.1.8 pod
-
 Phaseolus vulgaris
-
1.5.1.8 root
-
 Phaseolus vulgaris
-
1.5.1.9 cotyledon
-
 Phaseolus vulgaris
-
1.5.1.9 hypocotyl
-
 Phaseolus vulgaris
-
1.5.1.9 leaf
-
 Phaseolus vulgaris
-
1.5.1.9 pod very low activity Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.8 4.98
-
 lysine 2-oxoglutarate reductase activity Phaseolus vulgaris
1.5.1.9 16.8
-
 saccharopine dehydrogenase activity Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.8 L-Lys + 2-oxoglutarate + NADPH
-
 Phaseolus vulgaris N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
1.5.1.9 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
 Phaseolus vulgaris L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.8 LOR-SDH
-
 Phaseolus vulgaris
1.5.1.8 lysine 2-oxoglutarate reductase-saccharopine dehydrogenase bifunctional enzyme Phaseolus vulgaris
1.5.1.9 LOR-SDH
-
 Phaseolus vulgaris
1.5.1.9 lysine 2-oxoglutarate reductase-saccharopine dehydrogenase bifunctional enzyme Phaseolus vulgaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.8 NADPH
-
 Phaseolus vulgaris
1.5.1.9 NAD+
-
 Phaseolus vulgaris