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Literature summary extracted from

  • Sano, S.; Kang, Y.N.; Shigemizu, H.; Morishita, N.; Yoon, H.J.; Saito, K.; Asada, K.; Mikami, B.
    Crystallization and preliminary crystallographic analysis of monodehydroascorbate radical reductase from cucumber (2004), Acta Crystallogr. Sect. D, 60, 1498-1499.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.5.4 overexpression in Escherichia coli Cucumis sativus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.6.5.4 purified recombinant enzyme, immediately after purification by hanging drop vapour diffusion method, 0.005 ml of protein solution containing 10 mg/ml protein, mixed with equal volume of reservoir solution containing 20-25% PEG 6000, 0.1 M CaCl2, 50 mM Tris-HCl, pH 8.0, equilibration against 1 ml reservoir solution, 20°C, 1 week, cryoprotection by 30% glycerol, X-ray diffraction structure determination and analysis at 2.4 A resolution Cucumis sativus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.4 thiol-modifiying reagents inhibition at the step of FAD reduction by NADH producing a charge-transfer complex Cucumis sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.4 additional information
-
 additional information kinetics Cucumis sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.4 47300
-
 x * 47300, SDS-PAGE Cucumis sativus

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.4 Cucumis sativus
-
 cucumber
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.4 recombinant from Escherichia coli Cucumis sativus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.6.5.4 NADH + H+ + 2 monodehydroascorbate = NAD+ + 2 ascorbate ping-pong mechanism Cucumis sativus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.4 NAD(P)H + monodehydroascorbate reduction of the radical monodehydroascorbate, via FAD as intermediary electron acceptor, FAD reduction by NADH producing a charge-transfer complex Cucumis sativus NAD(P)+ + ascorbate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.5.4 ? x * 47300, SDS-PAGE Cucumis sativus

Synonyms

EC Number Synonyms Comment Organism
1.6.5.4 MDA
-
 Cucumis sativus
1.6.5.4 monodehydroascorbate radical reductase
-
 Cucumis sativus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.4 FAD
-
 Cucumis sativus
1.6.5.4 NAD(P)H
-
 Cucumis sativus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.6.5.4 Cucumis sativus sequence calculation
-
 6