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Literature summary extracted from

  • Yin, S.; Kiong Ho, C.; Miller, E.S.; Shuman, S.
    Characterization of bacteriophage KVP40 and T4 RNA ligase 2 (2004), Virology, 319, 141-151.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.3 expressed in Escherichia coli as a His10-tagged fusion protein Vibrio phage KVP40

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.3 42000
-
 adsorption to Ni-agarose and step-elution with imidazole Vibrio phage KVP40

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m Tequatrovirus T4
-
 AMP + diphosphate + (ribonucleotide)n+m
-
 ?
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m Vibrio phage KVP40 catalyzes RNA end-joining AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.3 Tequatrovirus T4
-
-
-
6.5.1.3 Vibrio phage KVP40
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.3 recombinant protein Vibrio phage KVP40

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m
-
 Tequatrovirus T4 AMP + diphosphate + (ribonucleotide)n+m
-
 ?
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m
-
 Vibrio phage KVP40 AMP + diphosphate + (ribonucleotide)n+m
-
 ?
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m catalyzes RNA end-joining Vibrio phage KVP40 AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Subunits

EC Number Subunits Comment Organism
6.5.1.3 monomer 1 * 42000, SDS-PAGE Vibrio phage KVP40

Synonyms

EC Number Synonyms Comment Organism
6.5.1.3 phage Rnl2
-
 Vibrio phage KVP40
6.5.1.3 RNA ligase (ATP)
-
 Vibrio phage KVP40
6.5.1.3 Rnl2
-
 Tequatrovirus T4
6.5.1.3 Rnl2
-
 Vibrio phage KVP40
6.5.1.3 T4 RNA ligase 2
-
 Tequatrovirus T4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.3 7
-
 adenylyltransferase activity Vibrio phage KVP40

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.5.1.3 5 9.5 activity is virtually nil at pH below 5.0 or above 9.5 Vibrio phage KVP40