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Literature summary extracted from

  • Shukla, G.S.; Chiu, J.; Hart, B.A.
    Enhanced expression of pulmonary gamma-glutamylcysteine synthetase heavy subunit in rats exposed to cadmium aerosols (2000), Toxicol. Appl. Pharmacol., 163, 249-259.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.2.2 cadmium aerosols 2.4 mg Cd/m3, enhance in the lung the expression of the enzyme's heavy, catalytic subunit gamma-GCS-HS by 4.5fold after 15 min, 8fold after 6 h, increase in enzyme activity and GSH production rate Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.2 L-buthionine-SR-sulfoximine specific Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.2 73000
-
 x * 73000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.2 ATP + L-Glu + L-Cys Rattus norvegicus first and rate limiting step in GSH de novo biosynthesis ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.2 Rattus norvegicus
-
 male Lewis rats
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.2 alveolus corners of, highest enzyme content Rattus norvegicus
-
6.3.2.2 epithelium alveolar, higher content compared to the lung parenchyma Rattus norvegicus
-
6.3.2.2 kidney
-
 Rattus norvegicus
-
6.3.2.2 lung
-
 Rattus norvegicus
-
6.3.2.2 additional information pulmonary distribution Rattus norvegicus
-
6.3.2.2 parenchyma lower content compared to the alveolar epithelium Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.2 0.0027
-
 lung extract exposed to air Rattus norvegicus
6.3.2.2 0.0041
-
 lung extract exposed to cadmium aerosol with 3.3 mg Cd/m3 Rattus norvegicus
6.3.2.2 0.0048
-
 lung extract exposed to cadmium aerosol with 4.2 mg Cd/m3 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.2 ATP + L-Glu + L-Cys
-
 Rattus norvegicus ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
6.3.2.2 ATP + L-Glu + L-Cys first and rate limiting step in GSH de novo biosynthesis Rattus norvegicus ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.2 ? x * 73000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
6.3.2.2 gamma-GCS
-
 Rattus norvegicus
6.3.2.2 gamma-Glutamylcysteine synthetase
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.2 ATP
-
 Rattus norvegicus