Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rath, V.L.; Silvian, L.F.; Beijer, B.; Sproat, B.S.; Steitz, T.A.
    How glutaminyl-tRNA synthetase selects glutamine (1998), Structure, 6, 439-449.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.18 cocrystallization of the purified enzyme with tRNAGln and inhibitor QSI, X-ray diffraction structure determination at 2.4 A resolution and analysis Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.18 5'-O-[N-(L-glutaminyl)sulfamoyl] adenosine i.e. QSI, glutaminyl-adenylate analogue, competitive to glutamine Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.18 ATP + L-glutamine + tRNAGln Escherichia coli
-
 AMP + diphosphate + L-glutaminyl-tRNAGln
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.18 Escherichia coli P00962
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.18 ATP + L-glutamine + tRNAGln = AMP + diphosphate + L-glutaminyl-tRNAGln aminoacylation reaction mechanism, substrate binding sites, Tyr211 and a water molecule are responsible for recognition of both hydrogen atoms of the nitrogen of glutamine sidechain, which is esstial for substrate recognition Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.18 ATP + L-glutamine + tRNAGln
-
 Escherichia coli AMP + diphosphate + L-glutaminyl-tRNAGln
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.18 GlnRS
-
 Escherichia coli
6.1.1.18 Glutaminyl-tRNA synthetase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.18 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.18 7
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.18 ATP
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.1.1.18 0.0013
-
 5'-O-[N-(L-glutaminyl)sulfamoyl] adenosine pH 7.0, 37°C Escherichia coli