BRENDA - Enzyme Database

Glutamyl endopeptidases: structure, function, and practical application

Mil'gotina, E.I.; Voyushina, T.L.; Chestukhina, G.G.; Russ. J. Bioorg. Chem. 29, 511-522 (2003)
No PubMed abstract available

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
3.4.21.19
analysis
study of structure-function organization of anti-HIV and antitumor proteins MAP30 and GAP31 by limited proteolysis with V8-GSE, enzym may be useful for studying other proteins as well
Staphylococcus aureus
3.4.21.19
synthesis
enzyme can be used as catalyst for peptide synthesis in hydrophilic organic solvents with low water content, e.g. acetonitrile, overview
Staphylococcus aureus
Crystallization (Commentary)
EC Number
Crystallization (Commentary)
Organism
3.4.21.19
enzyme is crystallized complexed with substrate Boc-Ala-Ala-Pro-Glu-4-nitroanilide, X-ray structure determination and analysis at 2.0 A resolution
Streptomyces griseus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.21.19
additional information
enzyme is a metalloprotease
Bacillus subtilis
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.4.21.19
Bacillus licheniformis
-
-
-
3.4.21.19
Bacillus subtilis
-
enzyme possesses Cys at position 193 instead of highly conserved Gly, the most conserved residue of the trypsin enzyme family
-
3.4.21.19
Staphylococcus aureus
-
-
-
3.4.21.19
Staphylococcus aureus V8
-
-
-
3.4.21.19
Streptomyces griseus
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
Reaction ID
3.4.21.19
Preferential cleavage: Glu-/-, Asp-/-
catalytic tetrad of His213, Asp102, Ser195, and Trp214, computer modeling of S1 and S'1 sites of the enzyme's active site
Staphylococcus aureus
3.4.21.19
Preferential cleavage: Glu-/-, Asp-/-
catalytic triad of His, Asp, and Ser
Bacillus subtilis
3.4.21.19
Preferential cleavage: Glu-/-, Asp-/-
catalytic triad of His, Asp, and Ser, computer modeling of active site structure, S1-substrate binding site, and substrate binding mechanism
Bacillus licheniformis
3.4.21.19
Preferential cleavage: Glu-/-, Asp-/-
catalytic triad of His, Asp, and Ser, substrate binding mechanism and structure
Streptomyces griseus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.4.21.19
benzyloxycarbonyl-Ala 2-carboxyphenylthioester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Staphylococcus aureus
?
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Ala 2-carboxyphenylthioester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Bacillus licheniformis
?
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Ala 2-carboxyphenylthioester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Staphylococcus aureus V8
?
-
653874
Staphylococcus aureus V8
?
3.4.21.19
benzyloxycarbonyl-Ala 3-carboxyphenylester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Staphylococcus aureus
?
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Ala 3-carboxyphenylester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Bacillus licheniformis
?
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Ala 3-carboxyphenylester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Staphylococcus aureus V8
?
-
653874
Staphylococcus aureus V8
?
3.4.21.19
benzyloxycarbonyl-Ala 4-carboxyphenylester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Staphylococcus aureus
?
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Ala 4-carboxyphenylester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Bacillus licheniformis
?
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Ala carboxyethylthioester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Staphylococcus aureus
?
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Ala carboxyethylthioester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Bacillus licheniformis
?
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Ala carboxyethylthioester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Staphylococcus aureus V8
?
-
653874
Staphylococcus aureus V8
?
3.4.21.19
benzyloxycarbonyl-Ala carboxymethylthioester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Staphylococcus aureus
?
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Ala carboxymethylthioester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Bacillus licheniformis
?
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Ala carboxymethylthioester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Staphylococcus aureus V8
?
-
653874
Staphylococcus aureus V8
?
3.4.21.19
benzyloxycarbonyl-Ala-Ala-Glu-methyl ester + leucine * HCl
peptide synthesis
653874
Staphylococcus aureus
benzyloxycarbonyl-Ala-Ala-Glu-Leu + methanol
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Ala-Glu-methyl ester + leucine * HCl
peptide synthesis
653874
Staphylococcus aureus
benzyloxycarbonyl-Ala-Glu-Leu + methanol
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Asp-methyl ester + Ala-Ala-D-hydroxyalanine
peptide synthesis, low activity at 25°C, but high activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Asp-Ala-Ala-D-hydroxyalanine + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Asp-methyl ester + Ala-Ala-hydroxyproline
peptide synthesis, lower activity at 25°C, but high activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Asp-Ala-Ala-hydroxyproline + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Asp-methyl ester + Ala-D-hydroxyalanine
peptide synthesis, very low activity at 25°C, but moderate activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Asp-Ala-D-hydroxyalanine + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Asp-methyl ester + Ala-hydroxyaspartate
peptide synthesis, low activity at 25°C, but high activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Asp-Ala-hydroxyaspartate + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Asp-methyl ester + Ala-hydroxyglutamate
peptide synthesis, very low activity at 25°C, but high activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Asp-Ala-hydroxyglutamate + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Asp-methyl ester + Ala-hydroxyproline
peptide synthesis, no activity at 25°C, only low activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Asp-Ala-hydroxyproline + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Asp-methyl ester + Asp-Gly
peptide synthesis, no activity at 25°C, but moderate activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Asp-Asp-Gly + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Asp-methyl ester + aspartate
peptide synthesis, no activity at 25°C, but high activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Asp-Asp + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Asp-methyl ester + Glu-Gly
peptide synthesis, no activity at 25°C, but moderate activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Asp-Glu-Gly + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Asp-methyl ester + glutamate
peptide synthesis, no activity at 25°C, but high activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Asp-Glu + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + Ala-Ala-D-Ala
peptide synthesis, low activity at 25°C, but high activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Glu-Ala-Ala-D-Ala + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + Ala-Ala-Pro
peptide synthesis, lower activity at 25°C, but high activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Glu-Ala-Ala-Pro + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + Ala-Asp
peptide synthesis, no activity at 25°C, but high activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Glu-Ala-Asp + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + Ala-D-Ala
peptide synthesis, no activity at 25°C, but moderate activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Glu-Ala-D-Ala + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + Ala-Glu
peptide synthesis, no activity at 25°C, but high activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Glu-Ala-Glu + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + Ala-Pro
peptide synthesis, no activity at 25°C, only low activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Glu-Ala-Pro + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + Asp-Gly
peptide synthesis, no activity at 25°C, but moderate activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Glu-Asp-Gly + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + Glu-Gly
peptide synthesis, no activity at 25°C, but low activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Glu-Glu-Gly + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + L-aspartate
peptide synthesis, no activity at 25°C, but moderate activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Glu-Asp + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + L-glutamate
peptide synthesis, no activity at 25°C, but moderate activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Glu-Glu + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + L-tryptophan
peptide synthesis
653874
Staphylococcus aureus
benzyloxycarbonyl-Glu-Trp + methanol
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + Leu-Gly
peptide synthesis
653874
Staphylococcus aureus
benzyloxycarbonyl-Glu-Leu-Gly + methanol
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + leucine
peptide synthesis
653874
Staphylococcus aureus
benzyloxycarbonyl-Glu-Leu + methanol
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + Phe-Gly
peptide synthesis
653874
Staphylococcus aureus
benzyloxycarbonyl-Glu-Phe-Gly + methanol
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Pro-Leu-Gly-S-CH2-COOH + LAFARAEAFG
acylation of peptide fragment by substrate mimetic
653874
Staphylococcus aureus
benzyloxycarbonyl-PLGLAFARAEAFG + HS-CH2-COOH
product formation 55%
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-S-CH2-COOH + LAFARAEAF-hydroxyglycine
acylation of peptide fragment by substrate mimetic
653874
Staphylococcus aureus
benzyloxycarbonyl-LAFARAEAF-hydroxyglycine + HS-CH2-COOH
product formation 99%
653874
Staphylococcus aureus
?
3.4.21.19
beta-type parvalbumin + H2O
from the frog Rana catesbeiana
653874
Staphylococcus aureus
peptide fragments
mass spectrometry for identification
653874
Staphylococcus aureus
?
3.4.21.19
bovine hemoglobin + H2O
in presence of SDS
653874
Staphylococcus aureus
peptide fragments
peptide mapping, 2 peptide fragments are Leu76-Pro-Gly-Ala-Leu-Ser-Glu82 and Lys94-Leu-His-Val-Asp-Pro-Glu100
653874
Staphylococcus aureus
?
3.4.21.19
hemocyanin + H2O
hydrolysis of 2 isozymes of hemocyanin KLH1 and KLH2 from shellfish Megatura crenulata at Glu-Xaa and Asp-Xaa bonds
653874
Staphylococcus aureus
peptide fragments
-
653874
Staphylococcus aureus
?
3.4.21.19
additional information
no peptide synthesis activity with proline and D-leucine, active in semienzymatic synthesis of human growth hormone
653874
Bacillus licheniformis
?
-
653874
Bacillus licheniformis
?
3.4.21.19
additional information
substrate specificity is pH-dependent due to active site His213, peptide synthesizing substrate specificity, no peptide synthesizing activity with benzyloxycarbonyl-Asp-methyl ester
653874
Staphylococcus aureus
?
-
653874
Staphylococcus aureus
?
3.4.21.19
additional information
substrate specificity is pH-dependent due to active site His213, peptide synthesizing substrate specificity, no peptide synthesizing activity with benzyloxycarbonyl-Asp-methyl ester
653874
Staphylococcus aureus V8
?
-
653874
Staphylococcus aureus V8
?
3.4.21.19
tert-butyloxycarbonyl-Ala-Ala-Pro-Glu-4-nitroanilide + H2O
-
653874
Streptomyces griseus
tert-butyloxycarbonyl-Ala-Ala-Pro-Glu + 4-nitroaniline
-
653874
Streptomyces griseus
?
Subunits
EC Number
Subunits
Commentary
Organism
3.4.21.19
More
structural and functional organization, overview, enzyme shows 2 beta-barrels and a C-terminal alpha-helix when complexed with substrate Boc-Ala-Ala-Pro-Glu-4-nitroanilide
Streptomyces griseus
3.4.21.19
More
structural and functional rganization, overview
Staphylococcus aureus
3.4.21.19
More
structural and functional rganization, overview
Bacillus subtilis
3.4.21.19
More
structural and functional rganization, overview
Bacillus licheniformis
Synonyms
EC Number
Synonyms
Commentary
Organism
3.4.21.19
BL-GSE
-
Bacillus licheniformis
3.4.21.19
BS-GSE
-
Bacillus subtilis
3.4.21.19
glutamic acid-specific endopeptidase
-
Staphylococcus aureus
3.4.21.19
glutamic acid-specific endopeptidase
-
Bacillus subtilis
3.4.21.19
glutamic acid-specific endopeptidase
-
Bacillus licheniformis
3.4.21.19
glutamic acid-specific endopeptidase
-
Streptomyces griseus
3.4.21.19
SG-GSE
-
Streptomyces griseus
3.4.21.19
V8 protease
-
Staphylococcus aureus
3.4.21.19
V8-GSE
-
Staphylococcus aureus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.4.21.19
additional information
-
peptide synthesis at -15°C
Bacillus licheniformis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.21.19
7.3
8
-
Bacillus licheniformis
3.4.21.19
9
-
-
Streptomyces griseus
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.4.21.19
Staphylococcus aureus
2 ionogenic groups
-
additional information
Application (protein specific)
EC Number
Application
Commentary
Organism
3.4.21.19
analysis
study of structure-function organization of anti-HIV and antitumor proteins MAP30 and GAP31 by limited proteolysis with V8-GSE, enzym may be useful for studying other proteins as well
Staphylococcus aureus
3.4.21.19
synthesis
enzyme can be used as catalyst for peptide synthesis in hydrophilic organic solvents with low water content, e.g. acetonitrile, overview
Staphylococcus aureus
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.4.21.19
enzyme is crystallized complexed with substrate Boc-Ala-Ala-Pro-Glu-4-nitroanilide, X-ray structure determination and analysis at 2.0 A resolution
Streptomyces griseus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.21.19
additional information
enzyme is a metalloprotease
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.4.21.19
benzyloxycarbonyl-Ala 2-carboxyphenylthioester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Staphylococcus aureus
?
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Ala 2-carboxyphenylthioester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Bacillus licheniformis
?
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Ala 2-carboxyphenylthioester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Staphylococcus aureus V8
?
-
653874
Staphylococcus aureus V8
?
3.4.21.19
benzyloxycarbonyl-Ala 3-carboxyphenylester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Staphylococcus aureus
?
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Ala 3-carboxyphenylester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Bacillus licheniformis
?
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Ala 3-carboxyphenylester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Staphylococcus aureus V8
?
-
653874
Staphylococcus aureus V8
?
3.4.21.19
benzyloxycarbonyl-Ala 4-carboxyphenylester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Staphylococcus aureus
?
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Ala 4-carboxyphenylester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Bacillus licheniformis
?
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Ala carboxyethylthioester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Staphylococcus aureus
?
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Ala carboxyethylthioester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Bacillus licheniformis
?
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Ala carboxyethylthioester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Staphylococcus aureus V8
?
-
653874
Staphylococcus aureus V8
?
3.4.21.19
benzyloxycarbonyl-Ala carboxymethylthioester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Staphylococcus aureus
?
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Ala carboxymethylthioester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Bacillus licheniformis
?
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Ala carboxymethylthioester + H2O
enzyme also performs acyl-transfer reaction with substrate mimetics
653874
Staphylococcus aureus V8
?
-
653874
Staphylococcus aureus V8
?
3.4.21.19
benzyloxycarbonyl-Ala-Ala-Glu-methyl ester + leucine * HCl
peptide synthesis
653874
Staphylococcus aureus
benzyloxycarbonyl-Ala-Ala-Glu-Leu + methanol
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Ala-Glu-methyl ester + leucine * HCl
peptide synthesis
653874
Staphylococcus aureus
benzyloxycarbonyl-Ala-Glu-Leu + methanol
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Asp-methyl ester + Ala-Ala-D-hydroxyalanine
peptide synthesis, low activity at 25°C, but high activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Asp-Ala-Ala-D-hydroxyalanine + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Asp-methyl ester + Ala-Ala-hydroxyproline
peptide synthesis, lower activity at 25°C, but high activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Asp-Ala-Ala-hydroxyproline + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Asp-methyl ester + Ala-D-hydroxyalanine
peptide synthesis, very low activity at 25°C, but moderate activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Asp-Ala-D-hydroxyalanine + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Asp-methyl ester + Ala-hydroxyaspartate
peptide synthesis, low activity at 25°C, but high activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Asp-Ala-hydroxyaspartate + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Asp-methyl ester + Ala-hydroxyglutamate
peptide synthesis, very low activity at 25°C, but high activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Asp-Ala-hydroxyglutamate + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Asp-methyl ester + Ala-hydroxyproline
peptide synthesis, no activity at 25°C, only low activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Asp-Ala-hydroxyproline + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Asp-methyl ester + Asp-Gly
peptide synthesis, no activity at 25°C, but moderate activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Asp-Asp-Gly + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Asp-methyl ester + aspartate
peptide synthesis, no activity at 25°C, but high activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Asp-Asp + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Asp-methyl ester + Glu-Gly
peptide synthesis, no activity at 25°C, but moderate activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Asp-Glu-Gly + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Asp-methyl ester + glutamate
peptide synthesis, no activity at 25°C, but high activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Asp-Glu + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + Ala-Ala-D-Ala
peptide synthesis, low activity at 25°C, but high activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Glu-Ala-Ala-D-Ala + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + Ala-Ala-Pro
peptide synthesis, lower activity at 25°C, but high activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Glu-Ala-Ala-Pro + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + Ala-Asp
peptide synthesis, no activity at 25°C, but high activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Glu-Ala-Asp + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + Ala-D-Ala
peptide synthesis, no activity at 25°C, but moderate activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Glu-Ala-D-Ala + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + Ala-Glu
peptide synthesis, no activity at 25°C, but high activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Glu-Ala-Glu + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + Ala-Pro
peptide synthesis, no activity at 25°C, only low activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Glu-Ala-Pro + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + Asp-Gly
peptide synthesis, no activity at 25°C, but moderate activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Glu-Asp-Gly + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + Glu-Gly
peptide synthesis, no activity at 25°C, but low activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Glu-Glu-Gly + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + L-aspartate
peptide synthesis, no activity at 25°C, but moderate activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Glu-Asp + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + L-glutamate
peptide synthesis, no activity at 25°C, but moderate activity at -15°C
653874
Bacillus licheniformis
benzyloxycarbonyl-Glu-Glu + methanol
-
653874
Bacillus licheniformis
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + L-tryptophan
peptide synthesis
653874
Staphylococcus aureus
benzyloxycarbonyl-Glu-Trp + methanol
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + Leu-Gly
peptide synthesis
653874
Staphylococcus aureus
benzyloxycarbonyl-Glu-Leu-Gly + methanol
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + leucine
peptide synthesis
653874
Staphylococcus aureus
benzyloxycarbonyl-Glu-Leu + methanol
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Glu-methyl ester + Phe-Gly
peptide synthesis
653874
Staphylococcus aureus
benzyloxycarbonyl-Glu-Phe-Gly + methanol
-
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-Pro-Leu-Gly-S-CH2-COOH + LAFARAEAFG
acylation of peptide fragment by substrate mimetic
653874
Staphylococcus aureus
benzyloxycarbonyl-PLGLAFARAEAFG + HS-CH2-COOH
product formation 55%
653874
Staphylococcus aureus
?
3.4.21.19
benzyloxycarbonyl-S-CH2-COOH + LAFARAEAF-hydroxyglycine
acylation of peptide fragment by substrate mimetic
653874
Staphylococcus aureus
benzyloxycarbonyl-LAFARAEAF-hydroxyglycine + HS-CH2-COOH
product formation 99%
653874
Staphylococcus aureus
?
3.4.21.19
beta-type parvalbumin + H2O
from the frog Rana catesbeiana
653874
Staphylococcus aureus
peptide fragments
mass spectrometry for identification
653874
Staphylococcus aureus
?
3.4.21.19
bovine hemoglobin + H2O
in presence of SDS
653874
Staphylococcus aureus
peptide fragments
peptide mapping, 2 peptide fragments are Leu76-Pro-Gly-Ala-Leu-Ser-Glu82 and Lys94-Leu-His-Val-Asp-Pro-Glu100
653874
Staphylococcus aureus
?
3.4.21.19
hemocyanin + H2O
hydrolysis of 2 isozymes of hemocyanin KLH1 and KLH2 from shellfish Megatura crenulata at Glu-Xaa and Asp-Xaa bonds
653874
Staphylococcus aureus
peptide fragments
-
653874
Staphylococcus aureus
?
3.4.21.19
additional information
no peptide synthesis activity with proline and D-leucine, active in semienzymatic synthesis of human growth hormone
653874
Bacillus licheniformis
?
-
653874
Bacillus licheniformis
?
3.4.21.19
additional information
substrate specificity is pH-dependent due to active site His213, peptide synthesizing substrate specificity, no peptide synthesizing activity with benzyloxycarbonyl-Asp-methyl ester
653874
Staphylococcus aureus
?
-
653874
Staphylococcus aureus
?
3.4.21.19
additional information
substrate specificity is pH-dependent due to active site His213, peptide synthesizing substrate specificity, no peptide synthesizing activity with benzyloxycarbonyl-Asp-methyl ester
653874
Staphylococcus aureus V8
?
-
653874
Staphylococcus aureus V8
?
3.4.21.19
tert-butyloxycarbonyl-Ala-Ala-Pro-Glu-4-nitroanilide + H2O
-
653874
Streptomyces griseus
tert-butyloxycarbonyl-Ala-Ala-Pro-Glu + 4-nitroaniline
-
653874
Streptomyces griseus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.4.21.19
More
structural and functional organization, overview, enzyme shows 2 beta-barrels and a C-terminal alpha-helix when complexed with substrate Boc-Ala-Ala-Pro-Glu-4-nitroanilide
Streptomyces griseus
3.4.21.19
More
structural and functional rganization, overview
Staphylococcus aureus
3.4.21.19
More
structural and functional rganization, overview
Bacillus subtilis
3.4.21.19
More
structural and functional rganization, overview
Bacillus licheniformis
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.4.21.19
additional information
-
peptide synthesis at -15°C
Bacillus licheniformis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.21.19
7.3
8
-
Bacillus licheniformis
3.4.21.19
9
-
-
Streptomyces griseus
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.4.21.19
Staphylococcus aureus
2 ionogenic groups
-
additional information