BRENDA - Enzyme Database

In vivo and in vitro processing of the Bacillus subtilis transcript coding for glutamyl-tRNA synthetase, serine acetyltransferase, and cysteinyl-tRNA synthetase

Pelchat, M.; Lapointe, J.; RNA 5, 281-289 (1999)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
6.1.1.16
the gene encoding the enzyme is expressed as a polycistronic gltX-cysE-cysS transcript, which is processed to a cysE-cysS transcript, regulatory implications
Bacillus subtilis
6.1.1.17
gene gltX is encoded in an operon comprising the genes gltX, cysE, and cysS, after transcription the polycistronic mRNA is cleaved into the gltX transcript and the cysE-cysS transcript due to an internal signal structure and probably by self-cleavage, analysis, the gltX mRNA may be stabilized by secondary structures at the 3'-end and the 5'-end, expression in Escherichia coli and in vitro transcription
Bacillus subtilis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.16
ATP + L-cysteine + tRNACys
Bacillus subtilis
-
AMP + diphosphate + L-cysteinyl-tRNACys
-
Bacillus subtilis
?
6.1.1.16
ATP + L-cysteine + tRNACys
Bacillus subtilis 168T
-
AMP + diphosphate + L-cysteinyl-tRNACys
-
Bacillus subtilis 168T
?
6.1.1.16
additional information
Bacillus subtilis
probable regulation of enzyme activity by processing of polycistronic mRNA
?
-
Bacillus subtilis
?
6.1.1.16
additional information
Bacillus subtilis 168T
probable regulation of enzyme activity by processing of polycistronic mRNA
?
-
Bacillus subtilis 168T
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Bacillus subtilis
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Bacillus subtilis
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Bacillus subtilis 168T
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Bacillus subtilis 168T
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.16
Bacillus subtilis
-
gene cysS
-
6.1.1.16
Bacillus subtilis 168T
-
gene cysS
-
6.1.1.17
Bacillus subtilis
-
-
-
6.1.1.17
Bacillus subtilis 168T
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.16
ATP + L-cysteine + tRNACys
-
653867
Bacillus subtilis
AMP + diphosphate + L-cysteinyl-tRNACys
-
653867
Bacillus subtilis
?
6.1.1.16
ATP + L-cysteine + tRNACys
-
653867
Bacillus subtilis 168T
AMP + diphosphate + L-cysteinyl-tRNACys
-
653867
Bacillus subtilis 168T
?
6.1.1.16
additional information
probable regulation of enzyme activity by processing of polycistronic mRNA
653867
Bacillus subtilis
?
-
653867
Bacillus subtilis
?
6.1.1.16
additional information
probable regulation of enzyme activity by processing of polycistronic mRNA
653867
Bacillus subtilis 168T
?
-
653867
Bacillus subtilis 168T
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
653867
Bacillus subtilis
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653867
Bacillus subtilis
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
653867
Bacillus subtilis 168T
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653867
Bacillus subtilis 168T
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
6.1.1.16
ATP
-
Bacillus subtilis
6.1.1.17
ATP
-
Bacillus subtilis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.16
the gene encoding the enzyme is expressed as a polycistronic gltX-cysE-cysS transcript, which is processed to a cysE-cysS transcript, regulatory implications
Bacillus subtilis
6.1.1.17
gene gltX is encoded in an operon comprising the genes gltX, cysE, and cysS, after transcription the polycistronic mRNA is cleaved into the gltX transcript and the cysE-cysS transcript due to an internal signal structure and probably by self-cleavage, analysis, the gltX mRNA may be stabilized by secondary structures at the 3'-end and the 5'-end, expression in Escherichia coli and in vitro transcription
Bacillus subtilis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
6.1.1.16
ATP
-
Bacillus subtilis
6.1.1.17
ATP
-
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.16
ATP + L-cysteine + tRNACys
Bacillus subtilis
-
AMP + diphosphate + L-cysteinyl-tRNACys
-
Bacillus subtilis
?
6.1.1.16
ATP + L-cysteine + tRNACys
Bacillus subtilis 168T
-
AMP + diphosphate + L-cysteinyl-tRNACys
-
Bacillus subtilis 168T
?
6.1.1.16
additional information
Bacillus subtilis
probable regulation of enzyme activity by processing of polycistronic mRNA
?
-
Bacillus subtilis
?
6.1.1.16
additional information
Bacillus subtilis 168T
probable regulation of enzyme activity by processing of polycistronic mRNA
?
-
Bacillus subtilis 168T
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Bacillus subtilis
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Bacillus subtilis
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Bacillus subtilis 168T
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Bacillus subtilis 168T
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.16
ATP + L-cysteine + tRNACys
-
653867
Bacillus subtilis
AMP + diphosphate + L-cysteinyl-tRNACys
-
653867
Bacillus subtilis
?
6.1.1.16
ATP + L-cysteine + tRNACys
-
653867
Bacillus subtilis 168T
AMP + diphosphate + L-cysteinyl-tRNACys
-
653867
Bacillus subtilis 168T
?
6.1.1.16
additional information
probable regulation of enzyme activity by processing of polycistronic mRNA
653867
Bacillus subtilis
?
-
653867
Bacillus subtilis
?
6.1.1.16
additional information
probable regulation of enzyme activity by processing of polycistronic mRNA
653867
Bacillus subtilis 168T
?
-
653867
Bacillus subtilis 168T
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
653867
Bacillus subtilis
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653867
Bacillus subtilis
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
653867
Bacillus subtilis 168T
AMP + diphosphate + L-glutamyl-tRNAGlu
-
653867
Bacillus subtilis 168T
?