BRENDA - Enzyme Database

Specific interaction of cytosolic and mitochondrial glyoxalase II with acidic phospholipids in form of liposomes results in the inhibition of the cytosolic enzyme only

Scire, A.; Tanfani, F.; Saccucci, F.; Bertoli, E.; Principato, G.; Proteins 41, 33-39 (2000) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.6
cardiolipin
negatively charged phospholipid, inhibits the cytosolic isozyme noncompetitively, specific ionic and probably also hydrophobic interaction
Homo sapiens
3.1.2.6
dioleoyl phosphatidic acid
i.e. DOPA, negatively charged phospholipid, strongly inhibits the cytosolic isozyme noncompetitively, specific ionic and probably also hydrophobic interaction
Homo sapiens
3.1.2.6
dipalmitoylphosphatidylserine
negatively charged phospholipid, inhibits the cytosolic isozyme noncompetitively, specific ionic and probably also hydrophobic interaction
Homo sapiens
3.1.2.6
additional information
negatively charged phospholipids, like dioleoyl phosphatidic acid, cardiolipin, and phosphatidylserine, specifically interact with the enzyme, but do not inhibit the mitochondrial isozyme, no effect by neutral phospholipids
Bos taurus
3.1.2.6
additional information
no effect by neutral phospholipids, phospholipid binding studies
Homo sapiens
3.1.2.6
phosphatidylserine
negatively charged phospholipid, inhibits the cytosolic isozyme noncompetitively, specific ionic and probably also hydrophobic interaction
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.6
additional information
-
additional information
kinetics in presence of liposomes consisting of different phospholipids
Homo sapiens
3.1.2.6
additional information
-
additional information
kinetics in presence of liposomes consisting of different phospholipids
Bos taurus
3.1.2.6
0.152
-
S-(2-hydroxyacyl)glutathione
cytosolic isozyme, pH 7.2, 25°C, in presence of dioleoyl phosphatidic acid
Homo sapiens
3.1.2.6
0.152
-
S-(2-hydroxyacyl)glutathione
mitochondrial isozyme, pH 7.2, 25°C
Bos taurus
3.1.2.6
0.162
-
S-(2-hydroxyacyl)glutathione
mitochondrial isozyme, pH 7.2, 25°C, in presence of dioleoyl phosphatidic acid
Bos taurus
3.1.2.6
0.169
-
S-(2-hydroxyacyl)glutathione
cytosolic isozyme, pH 7.2, 25°C, in presence of cardiolipin
Homo sapiens
3.1.2.6
0.191
-
S-(2-hydroxyacyl)glutathione
cytosolic isozyme, pH 7.2, 25°C, in presence of dipalmitoylphosphatidylserine
Homo sapiens
3.1.2.6
0.191
-
S-(2-hydroxyacyl)glutathione
mitochondrial isozyme, pH 7.2, 25°C, in presence of cardiolipin
Bos taurus
3.1.2.6
0.201
-
S-(2-hydroxyacyl)glutathione
cytosolic isozyme, pH 7.2, 25°C
Homo sapiens
3.1.2.6
0.255
-
S-(2-hydroxyacyl)glutathione
mitochondrial isozyme, pH 7.2, 25°C, in presence of phosphatidylserine
Bos taurus
3.1.2.6
0.283
-
S-(2-hydroxyacyl)glutathione
mitochondrial isozyme, pH 7.2, 25°C, in presence of dipalmitoylphosphatidylserine
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.2.6
cytosol
cytosolic isozyme
Homo sapiens
5829
-
3.1.2.6
mitochondrion
mitochondrial isozyme
Bos taurus
5739
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.1.2.6
S-(2-hydroxyacyl)glutathione + H2O
Homo sapiens
enzyme is part of the glyoxalase system and involved in detoxification of methylglyoxal
glutathione + a 2-hydroxycarboxylate anion
-
Homo sapiens
?
3.1.2.6
S-(2-hydroxyacyl)glutathione + H2O
Bos taurus
enzyme is part of the glyoxalase system and involved in detoxification of methylglyoxal
glutathione + a 2-hydroxycarboxylate anion
-
Bos taurus
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.1.2.6
Bos taurus
-
mitochondrial isozyme
-
3.1.2.6
Homo sapiens
-
recombinant cytosolic isozyme expressed in Escherichia coli
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.6
liver
-
Homo sapiens
-
3.1.2.6
liver
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.1.2.6
S-(2-hydroxyacyl)glutathione + H2O
-
653833
Homo sapiens
glutathione + a 2-hydroxycarboxylate anion
-
653833
Homo sapiens
?
3.1.2.6
S-(2-hydroxyacyl)glutathione + H2O
-
653833
Bos taurus
glutathione + a 2-hydroxycarboxylate anion
-
653833
Bos taurus
?
3.1.2.6
S-(2-hydroxyacyl)glutathione + H2O
enzyme is part of the glyoxalase system and involved in detoxification of methylglyoxal
653833
Homo sapiens
glutathione + a 2-hydroxycarboxylate anion
-
653833
Homo sapiens
?
3.1.2.6
S-(2-hydroxyacyl)glutathione + H2O
enzyme is part of the glyoxalase system and involved in detoxification of methylglyoxal
653833
Bos taurus
glutathione + a 2-hydroxycarboxylate anion
-
653833
Bos taurus
?
Synonyms
EC Number
Synonyms
Commentary
Organism
3.1.2.6
acetoacetylglutathione hydrolase
-
Homo sapiens
3.1.2.6
acetoacetylglutathione hydrolase
-
Bos taurus
3.1.2.6
Germ cell specific protein
-
Homo sapiens
3.1.2.6
Germ cell specific protein
-
Bos taurus
3.1.2.6
GLO II
-
Homo sapiens
3.1.2.6
GLO II
-
Bos taurus
3.1.2.6
glyoxalase II
-
Homo sapiens
3.1.2.6
glyoxalase II
-
Bos taurus
3.1.2.6
hydrolase, acetoacetylglutathione
-
Homo sapiens
3.1.2.6
hydrolase, acetoacetylglutathione
-
Bos taurus
3.1.2.6
hydrolase, hydroxyacylglutathione
-
Homo sapiens
3.1.2.6
hydrolase, hydroxyacylglutathione
-
Bos taurus
3.1.2.6
Round spermatid protein RSP29
-
Homo sapiens
3.1.2.6
Round spermatid protein RSP29
-
Bos taurus
3.1.2.6
S-2-hydroxylacylglutathione hydrolase
-
Homo sapiens
3.1.2.6
S-2-hydroxylacylglutathione hydrolase
-
Bos taurus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.2.6
25
-
assay at
Homo sapiens
3.1.2.6
25
-
assay at
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.6
7.2
-
assay at
Homo sapiens
3.1.2.6
7.2
-
assay at
Bos taurus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.2.6
0.0002
-
dioleoyl phosphatidic acid
cytosolic isozyme, pH 7.2, 25°C
Homo sapiens
3.1.2.6
0.0022
-
dipalmitoylphosphatidylserine
cytosolic isozyme, pH 7.2, 25°C
Homo sapiens
3.1.2.6
0.006
-
cardiolipin
cytosolic isozyme, pH 7.2, 25°C
Homo sapiens
3.1.2.6
0.055
-
phosphatidylserine
cytosolic isozyme, pH 7.2, 25°C
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.6
cardiolipin
negatively charged phospholipid, inhibits the cytosolic isozyme noncompetitively, specific ionic and probably also hydrophobic interaction
Homo sapiens
3.1.2.6
dioleoyl phosphatidic acid
i.e. DOPA, negatively charged phospholipid, strongly inhibits the cytosolic isozyme noncompetitively, specific ionic and probably also hydrophobic interaction
Homo sapiens
3.1.2.6
dipalmitoylphosphatidylserine
negatively charged phospholipid, inhibits the cytosolic isozyme noncompetitively, specific ionic and probably also hydrophobic interaction
Homo sapiens
3.1.2.6
additional information
negatively charged phospholipids, like dioleoyl phosphatidic acid, cardiolipin, and phosphatidylserine, specifically interact with the enzyme, but do not inhibit the mitochondrial isozyme, no effect by neutral phospholipids
Bos taurus
3.1.2.6
additional information
no effect by neutral phospholipids, phospholipid binding studies
Homo sapiens
3.1.2.6
phosphatidylserine
negatively charged phospholipid, inhibits the cytosolic isozyme noncompetitively, specific ionic and probably also hydrophobic interaction
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.2.6
0.0002
-
dioleoyl phosphatidic acid
cytosolic isozyme, pH 7.2, 25°C
Homo sapiens
3.1.2.6
0.0022
-
dipalmitoylphosphatidylserine
cytosolic isozyme, pH 7.2, 25°C
Homo sapiens
3.1.2.6
0.006
-
cardiolipin
cytosolic isozyme, pH 7.2, 25°C
Homo sapiens
3.1.2.6
0.055
-
phosphatidylserine
cytosolic isozyme, pH 7.2, 25°C
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.6
additional information
-
additional information
kinetics in presence of liposomes consisting of different phospholipids
Homo sapiens
3.1.2.6
additional information
-
additional information
kinetics in presence of liposomes consisting of different phospholipids
Bos taurus
3.1.2.6
0.152
-
S-(2-hydroxyacyl)glutathione
cytosolic isozyme, pH 7.2, 25°C, in presence of dioleoyl phosphatidic acid
Homo sapiens
3.1.2.6
0.152
-
S-(2-hydroxyacyl)glutathione
mitochondrial isozyme, pH 7.2, 25°C
Bos taurus
3.1.2.6
0.162
-
S-(2-hydroxyacyl)glutathione
mitochondrial isozyme, pH 7.2, 25°C, in presence of dioleoyl phosphatidic acid
Bos taurus
3.1.2.6
0.169
-
S-(2-hydroxyacyl)glutathione
cytosolic isozyme, pH 7.2, 25°C, in presence of cardiolipin
Homo sapiens
3.1.2.6
0.191
-
S-(2-hydroxyacyl)glutathione
cytosolic isozyme, pH 7.2, 25°C, in presence of dipalmitoylphosphatidylserine
Homo sapiens
3.1.2.6
0.191
-
S-(2-hydroxyacyl)glutathione
mitochondrial isozyme, pH 7.2, 25°C, in presence of cardiolipin
Bos taurus
3.1.2.6
0.201
-
S-(2-hydroxyacyl)glutathione
cytosolic isozyme, pH 7.2, 25°C
Homo sapiens
3.1.2.6
0.255
-
S-(2-hydroxyacyl)glutathione
mitochondrial isozyme, pH 7.2, 25°C, in presence of phosphatidylserine
Bos taurus
3.1.2.6
0.283
-
S-(2-hydroxyacyl)glutathione
mitochondrial isozyme, pH 7.2, 25°C, in presence of dipalmitoylphosphatidylserine
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.2.6
cytosol
cytosolic isozyme
Homo sapiens
5829
-
3.1.2.6
mitochondrion
mitochondrial isozyme
Bos taurus
5739
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.1.2.6
S-(2-hydroxyacyl)glutathione + H2O
Homo sapiens
enzyme is part of the glyoxalase system and involved in detoxification of methylglyoxal
glutathione + a 2-hydroxycarboxylate anion
-
Homo sapiens
?
3.1.2.6
S-(2-hydroxyacyl)glutathione + H2O
Bos taurus
enzyme is part of the glyoxalase system and involved in detoxification of methylglyoxal
glutathione + a 2-hydroxycarboxylate anion
-
Bos taurus
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.6
liver
-
Homo sapiens
-
3.1.2.6
liver
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.1.2.6
S-(2-hydroxyacyl)glutathione + H2O
-
653833
Homo sapiens
glutathione + a 2-hydroxycarboxylate anion
-
653833
Homo sapiens
?
3.1.2.6
S-(2-hydroxyacyl)glutathione + H2O
-
653833
Bos taurus
glutathione + a 2-hydroxycarboxylate anion
-
653833
Bos taurus
?
3.1.2.6
S-(2-hydroxyacyl)glutathione + H2O
enzyme is part of the glyoxalase system and involved in detoxification of methylglyoxal
653833
Homo sapiens
glutathione + a 2-hydroxycarboxylate anion
-
653833
Homo sapiens
?
3.1.2.6
S-(2-hydroxyacyl)glutathione + H2O
enzyme is part of the glyoxalase system and involved in detoxification of methylglyoxal
653833
Bos taurus
glutathione + a 2-hydroxycarboxylate anion
-
653833
Bos taurus
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.2.6
25
-
assay at
Homo sapiens
3.1.2.6
25
-
assay at
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.6
7.2
-
assay at
Homo sapiens
3.1.2.6
7.2
-
assay at
Bos taurus