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Literature summary extracted from

  • Scire, A.; Tanfani, F.; Saccucci, F.; Bertoli, E.; Principato, G.
    Specific interaction of cytosolic and mitochondrial glyoxalase II with acidic phospholipids in form of liposomes results in the inhibition of the cytosolic enzyme only (2000), Proteins, 41, 33-39.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.6 cardiolipin negatively charged phospholipid, inhibits the cytosolic isozyme noncompetitively, specific ionic and probably also hydrophobic interaction Homo sapiens
3.1.2.6 dioleoyl phosphatidic acid i.e. DOPA, negatively charged phospholipid, strongly inhibits the cytosolic isozyme noncompetitively, specific ionic and probably also hydrophobic interaction Homo sapiens
3.1.2.6 dipalmitoylphosphatidylserine negatively charged phospholipid, inhibits the cytosolic isozyme noncompetitively, specific ionic and probably also hydrophobic interaction Homo sapiens
3.1.2.6 additional information negatively charged phospholipids, like dioleoyl phosphatidic acid, cardiolipin, and phosphatidylserine, specifically interact with the enzyme, but do not inhibit the mitochondrial isozyme, no effect by neutral phospholipids Bos taurus
3.1.2.6 additional information no effect by neutral phospholipids, phospholipid binding studies Homo sapiens
3.1.2.6 phosphatidylserine negatively charged phospholipid, inhibits the cytosolic isozyme noncompetitively, specific ionic and probably also hydrophobic interaction Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.6 additional information
-
 additional information kinetics in presence of liposomes consisting of different phospholipids Homo sapiens
3.1.2.6 additional information
-
 additional information kinetics in presence of liposomes consisting of different phospholipids Bos taurus
3.1.2.6 0.152
-
 S-(2-hydroxyacyl)glutathione cytosolic isozyme, pH 7.2, 25°C, in presence of dioleoyl phosphatidic acid Homo sapiens
3.1.2.6 0.152
-
 S-(2-hydroxyacyl)glutathione mitochondrial isozyme, pH 7.2, 25°C Bos taurus
3.1.2.6 0.162
-
 S-(2-hydroxyacyl)glutathione mitochondrial isozyme, pH 7.2, 25°C, in presence of dioleoyl phosphatidic acid Bos taurus
3.1.2.6 0.169
-
 S-(2-hydroxyacyl)glutathione cytosolic isozyme, pH 7.2, 25°C, in presence of cardiolipin Homo sapiens
3.1.2.6 0.191
-
 S-(2-hydroxyacyl)glutathione cytosolic isozyme, pH 7.2, 25°C, in presence of dipalmitoylphosphatidylserine Homo sapiens
3.1.2.6 0.191
-
 S-(2-hydroxyacyl)glutathione mitochondrial isozyme, pH 7.2, 25°C, in presence of cardiolipin Bos taurus
3.1.2.6 0.201
-
 S-(2-hydroxyacyl)glutathione cytosolic isozyme, pH 7.2, 25°C Homo sapiens
3.1.2.6 0.255
-
 S-(2-hydroxyacyl)glutathione mitochondrial isozyme, pH 7.2, 25°C, in presence of phosphatidylserine Bos taurus
3.1.2.6 0.283
-
 S-(2-hydroxyacyl)glutathione mitochondrial isozyme, pH 7.2, 25°C, in presence of dipalmitoylphosphatidylserine Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.6 cytosol cytosolic isozyme Homo sapiens 5829
-
3.1.2.6 mitochondrion mitochondrial isozyme Bos taurus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.6 S-(2-hydroxyacyl)glutathione + H2O Homo sapiens enzyme is part of the glyoxalase system and involved in detoxification of methylglyoxal glutathione + a 2-hydroxycarboxylate anion
-
 ?
3.1.2.6 S-(2-hydroxyacyl)glutathione + H2O Bos taurus enzyme is part of the glyoxalase system and involved in detoxification of methylglyoxal glutathione + a 2-hydroxycarboxylate anion
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.6 Bos taurus
-
 mitochondrial isozyme
-
3.1.2.6 Homo sapiens
-
 recombinant cytosolic isozyme expressed in Escherichia coli
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.6 liver
-
 Homo sapiens
-
3.1.2.6 liver
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.6 S-(2-hydroxyacyl)glutathione + H2O
-
 Homo sapiens glutathione + a 2-hydroxycarboxylate anion
-
 ?
3.1.2.6 S-(2-hydroxyacyl)glutathione + H2O
-
 Bos taurus glutathione + a 2-hydroxycarboxylate anion
-
 ?
3.1.2.6 S-(2-hydroxyacyl)glutathione + H2O enzyme is part of the glyoxalase system and involved in detoxification of methylglyoxal Homo sapiens glutathione + a 2-hydroxycarboxylate anion
-
 ?
3.1.2.6 S-(2-hydroxyacyl)glutathione + H2O enzyme is part of the glyoxalase system and involved in detoxification of methylglyoxal Bos taurus glutathione + a 2-hydroxycarboxylate anion
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.6 acetoacetylglutathione hydrolase
-
 Homo sapiens
3.1.2.6 acetoacetylglutathione hydrolase
-
 Bos taurus
3.1.2.6 Germ cell specific protein
-
 Homo sapiens
3.1.2.6 Germ cell specific protein
-
 Bos taurus
3.1.2.6 GLO II
-
 Homo sapiens
3.1.2.6 GLO II
-
 Bos taurus
3.1.2.6 glyoxalase II
-
 Homo sapiens
3.1.2.6 glyoxalase II
-
 Bos taurus
3.1.2.6 hydrolase, acetoacetylglutathione
-
 Homo sapiens
3.1.2.6 hydrolase, acetoacetylglutathione
-
 Bos taurus
3.1.2.6 hydrolase, hydroxyacylglutathione
-
 Homo sapiens
3.1.2.6 hydrolase, hydroxyacylglutathione
-
 Bos taurus
3.1.2.6 Round spermatid protein RSP29
-
 Homo sapiens
3.1.2.6 Round spermatid protein RSP29
-
 Bos taurus
3.1.2.6 S-2-hydroxylacylglutathione hydrolase
-
 Homo sapiens
3.1.2.6 S-2-hydroxylacylglutathione hydrolase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.6 25
-
 assay at Homo sapiens
3.1.2.6 25
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.6 7.2
-
 assay at Homo sapiens
3.1.2.6 7.2
-
 assay at Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.2.6 0.0002
-
 dioleoyl phosphatidic acid cytosolic isozyme, pH 7.2, 25°C Homo sapiens
3.1.2.6 0.0022
-
 dipalmitoylphosphatidylserine cytosolic isozyme, pH 7.2, 25°C Homo sapiens
3.1.2.6 0.006
-
 cardiolipin cytosolic isozyme, pH 7.2, 25°C Homo sapiens
3.1.2.6 0.055
-
 phosphatidylserine cytosolic isozyme, pH 7.2, 25°C Homo sapiens