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Literature summary extracted from

  • Chen, Y.S.; Chien, C.H.; Goparaju, C.M.; Hsu, J.T.; Liang, P.H.; Chen, X.
    Purification and characterization of human prolyl dipeptidase DPP8 in Sf9 insect cells (2004), Protein Expr. Purif., 35, 142-146.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.13.18 expression in Sf9 insect cells Homo sapiens
3.4.13.18 overexpression in baculovirus infected Sf9 cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.13.18 0.12
-
 Ala-Pro-p-nitroanilide
-
 Homo sapiens
3.4.13.18 0.31
-
 Arg-Pro-p-nitroanilide
-
 Homo sapiens
3.4.13.18 0.48
-
 Gly-Pro-p-nitroanilide
-
 Homo sapiens
3.4.13.18 1.42
-
 Ala-Ala-p-nitroanilide
-
 Homo sapiens
3.4.13.18 2.05
-
 Asp-Pro-p-nitroanilide
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.13.18 soluble
-
 Homo sapiens
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.18 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.13.18
-
 Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.13.18 3 8
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.18 Ala-Ala-p-nitroanilide + H2O
-
 Homo sapiens Ala-Ala + p-nitroaniline
-
 ?
3.4.13.18 Ala-Pro-p-nitroanilide + H2O
-
 Homo sapiens Ala-Pro + p-nitroaniline
-
 ?
3.4.13.18 Arg-Pro-p-nitroanilide + H2O
-
 Homo sapiens Arg-Pro + p-nitroaniline
-
 ?
3.4.13.18 Asp-Pro-p-nitroanilide + H2O
-
 Homo sapiens Asp-Pro + p-nitroaniline
-
 ?
3.4.13.18 Gly-Pro-p-nitroanilide + H2O
-
 Homo sapiens Gly-Pro + p-nitroaniline
-
 ?
3.4.13.18 additional information the enzyme catalyzes cleavage at the carboxyl side of the proline residue at the penultimate position Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.13.18 DPP8
-
 Homo sapiens
3.4.13.18 prolyl dipeptidase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.13.18 0.7
-
 Ala-Ala-p-nitroanilide
-
 Homo sapiens
3.4.13.18 1.6
-
 Asp-Pro-p-nitroanilide
-
 Homo sapiens
3.4.13.18 12.8
-
 Arg-Pro-p-nitroanilide
-
 Homo sapiens
3.4.13.18 40
-
 Ala-Pro-p-nitroanilide
-
 Homo sapiens
3.4.13.18 62
-
 Gly-Pro-p-nitroanilide
-
 Homo sapiens