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Literature summary extracted from

  • Aich, S.; Imabayashi, F.; Delbaere, L.T.J.
    Expression, purification, and characterization of a bacterial GTP-dependent PEP carboxykinase (2003), Protein Expr. Purif., 31, 298-304.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.32 pckA gene, overexpression in Escherichia coli HG89 Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.32 Cr3+
-
 Corynebacterium glutamicum
4.1.1.32 additional information not inhibited by dithiothreitol Corynebacterium glutamicum
4.1.1.32 Zn2+
-
 Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.32 additional information
-
 additional information Km for NaHCO3: 5.48 mM Corynebacterium glutamicum
4.1.1.32 0.05
-
 GDP pH 6.8 Corynebacterium glutamicum
4.1.1.32 0.41
-
 phosphoenolpyruvate pH 6.8 Corynebacterium glutamicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.32 soluble recombinant enzyme Corynebacterium glutamicum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.32 Ba2+ sligthly activates Corynebacterium glutamicum
4.1.1.32 Co2+ significantly activates Corynebacterium glutamicum
4.1.1.32 Mg2+ absolute requirement of a metal ion, activity is maximal in the presence of both Mn2+ and Mg2+, for single ion activation Mn2+ can be substituted by Mg2+ with a reduced enzyme activity Corynebacterium glutamicum
4.1.1.32 Mn2+ absolute requirement of a metal ion, activity is maximal in the presence of both Mn2+ and Mg2+, for single ion activation Mn2+ can be substituted by Mg2+ with a reduced enzyme activity Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.32 67512
-
 1 * 67512, calculated from the amino acid sequence Corynebacterium glutamicum
4.1.1.32 67540
-
 recombinant enzyme, mass spectrometry Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.32 GTP + oxaloacetate Corynebacterium glutamicum key rate-limiting step in the gluconeogenic pathway, plays a role in the production of glutamine and lysine through the TCA cycle intermediates GDP + phosphoenolpyruvate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.32 Corynebacterium glutamicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.32 7.5fold, recombinant enzyme Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.32 additional information
-
-
 Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.32 GTP + oxaloacetate GTP-specific Corynebacterium glutamicum GDP + phosphoenolpyruvate + CO2
-
 r
4.1.1.32 GTP + oxaloacetate key rate-limiting step in the gluconeogenic pathway, plays a role in the production of glutamine and lysine through the TCA cycle intermediates Corynebacterium glutamicum GDP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.32 ITP + oxaloacetate lower activity with IDP than with GDP in the reverse reaction Corynebacterium glutamicum IDP + phosphoenolpyruvate + CO2
-
 r
4.1.1.32 additional information reverse reaction: not ADP Corynebacterium glutamicum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.32 monomer 1 * 67512, calculated from the amino acid sequence Corynebacterium glutamicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.32 additional information
-
 assay at room temperature Corynebacterium glutamicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.32 additional information
-
 additional information kcat for NaHCO3: 6.7 s-1 Corynebacterium glutamicum
4.1.1.32 6.1
-
 phosphoenolpyruvate pH 6.8 Corynebacterium glutamicum
4.1.1.32 7.2
-
 GDP pH 6.8 Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.32 6.8
-
-
 Corynebacterium glutamicum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.32 Corynebacterium glutamicum isoelectric focusing
-
 4.5
4.1.1.32 Corynebacterium glutamicum theoretically calculated pI
-
 4.61