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Literature summary extracted from

  • Alam, M.; Ho, S.; Vance, D.E.; Lehner, R.
    Heterologous expression, purification, and characterization of human triacylglycerol hydrolase (2002), Protein Expr. Purif., 24, 33-42.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 DNA and amino acid sequence determination and analysis, expression in Escherichia coli M15 with a N-terminal His-tagged substituting the signal sequence, the expressed protein is insoluble and inactive, expression of the C-terminally His-tagged enzyme containing its native signal sequence in Spodoptera frugiperda Sf9 insect cells via baculovirus infection results in active enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.3 4-nitrophenyl laurate + H2O Homo sapiens recombinant enzyme in Sf9 cells 4-nitrophenol + laurate
-
 ?
3.1.1.3 additional information Homo sapiens the enzyme mobilizes stored triacylglycerols some of which is used for very-low-density lipoprotein assembly in the liver ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.3 glycoprotein the recombinant enzyme from Sf9 insect cells becomes glycosylated in the endoplasmic reticulum Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 recombinant from Sf9 insect cells, about 30fold Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Homo sapiens 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O recombinant enzyme in Sf9 cells Homo sapiens 4-nitrophenol + laurate
-
 ?
3.1.1.3 additional information the enzyme mobilizes stored triacylglycerols some of which is used for very-low-density lipoprotein assembly in the liver Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 More enzyme belongs to the carboxylesterase family Homo sapiens
3.1.1.3 TGH
-
 Homo sapiens
3.1.1.3 triacylglycerol hydrolase
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
 assay at Homo sapiens