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Literature summary extracted from

  • Weber, B.; Hopwood, J.J.; Yogalingam, G.
    Expression and characterization of human recombinant and alpha-N-acetylglucosaminidase (2001), Protein Expr. Purif., 21, 251-259.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.50 medicine the use of secreted enzyme in future enzyme and gene replacement therapy protocols will by severely limited due to its small degree of mannose-6-phosphorylation Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.50 expression in CHO-K1 cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.50 0.0166
-
 O-(alpha-acetamido-2-deoxy-D-glucopyranosyl)-(1-3)-[L-6,3H]idoronic acid pH 4.5, 37°C, with bovine serum albumin Homo sapiens
3.2.1.50 1.07
-
 4-methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside pH 4.5, 37°C, without bovine serum albumin Homo sapiens
3.2.1.50 5.34
-
 4-methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside pH 4.5, 37°C, with bovine serum albumin Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.50 79000
-
 x * 79000, two isoforms of recombinant enzyme with MW of 89000 Da and 79000 Da differ in their glycosylation pattern, SDS-PAGE Homo sapiens
3.2.1.50 89000
-
 x * 89000, two isoforms of recombinant enzyme with MW of 89000 Da and 79000 Da differ in their glycosylation pattern, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.50 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.50 glycoprotein two isoforms of recombinant enzyme with MW of 89000 Da and 79000 Da differ in their glycosylation pattern Homo sapiens
3.2.1.50 phosphoprotein the specific phosphorylation of recombinant enzyme secreted from transfected CHO cells is significantly lower when compared with a control lysosomal enzyme Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.50 recombinant enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.50 culture medium of cells CHO cells stable transfected Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.50 1.057
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.50 4-methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside + H2O
-
 Homo sapiens ?
-
 ?
3.2.1.50 O-(alpha-acetamido-2-deoxy-D-glucopyranosyl)-(1-3)-[L-6,3H]idoronic acid + H2O
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.50 ? x * 79000, two isoforms of recombinant enzyme with MW of 89000 Da and 79000 Da differ in their glycosylation pattern, SDS-PAGE Homo sapiens
3.2.1.50 ? x * 89000, two isoforms of recombinant enzyme with MW of 89000 Da and 79000 Da differ in their glycosylation pattern, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.50 NAGLU
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.50 4.6
-
 reaction 4-methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside Homo sapiens