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Literature summary extracted from

  • Yoon, H.J.; Hashimoto, W.; Miyake, O.; Okamoto, M.; Mikami, B.; Murata, K.
    Overexpression in Escherichia coli, purification, and characterization of Sphingomonas sp. A1 alginate lyases (2000), Protein Expr. Purif., 19, 84-90.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.2.3 additional information isozymes A1-I and A1-II are not affected by addition of 1 mM EDTA, DTT, GSH, 2-mercaptoethanol, iodoacetic acid, and sugars, L-fucose, D-galactose, D-glucose, D-glucuronic acid, D-mannose, L-rhamnose, and D-xylose, no effect on isozymes A1-I and A1-II of Ca2+, Co2+, Mg2+, and Mn2+ at 1 mM Sphingomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 the 3 isozymes are natively expressed as 1 precursor molecule and are posttranslationally processed to monomeric forms, overexpression of all isozymes A1-I, A1-II, and A1-III in Escherichia coli Sphingomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.3 Hg2+ 40% inhibition at 1 mM of isozyme A1-I and A1-II Sphingomonas sp.
4.2.2.3 additional information isozymes A1-I and A1-II are not affected by addition of 1 mM EDTA, DTT, GSH, 2-mercaptoethanol, iodoacetic acid, and sugars, L-fucose, D-galactose, D-glucose, D-glucuronic acid, D-mannose, L-rhamnose, and D-xylose, no effect on isozymes A1-I and A1-II of Ca2+, Co2+, Mg2+, and Mn2+ at 1 mM Sphingomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 25000
-
 isozyme A1-II, gel filtration Sphingomonas sp.
4.2.2.3 40000
-
 isozyme A1-III, gel filtration Sphingomonas sp.
4.2.2.3 63000
-
 1 * 63000, A1-I, SDS-PAGE, 1 * 25000, A1-II, SDS-PAGE, 1 * 40000, A1-III, SDS-PAGE Sphingomonas sp.
4.2.2.3 66000
-
 isozyme A1-I, gel filtration Sphingomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Sphingomonas sp.
-
 isoforms A1-I, A1-II, and A1-III
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.2.3 proteolytic modification A1-I is autolytically cleaved into A1-II and A1-III, which shows different substrate specificities, therfore A1-I possesses 3 active sites, 1 protease and 2 alginate lyase sites Sphingomonas sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3 recombinant isozymes A1-I 11.2fold, A1-II 25.5fold, and A1-III 4.9fold, from Escherichia coli Sphingomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.3 45
-
 purified isozyme A1-III Sphingomonas sp.
4.2.2.3 73.1
-
 purified isozyme A1-I Sphingomonas sp.
4.2.2.3 109
-
 purified isozyme A1-II Sphingomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alginate isozyme A1-II and A1-I: endolytic cleavage of the glycosidic bonds, beta-elimination reaction, A1-II shows a preference for polyguluronate instead of polymannuronate Sphingomonas sp. unsaturated algino-oligosaccharides A1-I produces mainly di- and trisaccharides, A1-II produces mainly tri- and tetrasaccharides ?
4.2.2.3 additional information no activity with gellan, xanthan, and pectin of A1-I and A1-II Sphingomonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 monomer 1 * 63000, A1-I, SDS-PAGE, 1 * 25000, A1-II, SDS-PAGE, 1 * 40000, A1-III, SDS-PAGE Sphingomonas sp.

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 alginate lyase
-
 Sphingomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 25
-
 assay at Sphingomonas sp.
4.2.2.3 45
-
 isozyme A1-I Sphingomonas sp.
4.2.2.3 70
-
 isozyme A1-I Sphingomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.3 45
-
 pH 7.2, 10 min, loss f 50% activity, isozyme A1-I Sphingomonas sp.
4.2.2.3 50
-
 pH 7.2, 10 min, loss f 50% activity, isozyme A1-I Sphingomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 7.2
-
 assay at Sphingomonas sp.
4.2.2.3 8
-
 isozymes A1-I and A1-II Sphingomonas sp.