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Literature summary extracted from

  • Sheffield, P.J.; McMullen, T.W.; Li, J.; Ho, Y.S.; Garrard, S.M.; Derewenda, U.; Derewenda, Z.S.
    Preparation and crystal structure of the recombinant alpha(1)/alpha(2) catalytic heterodimer of bovine brain platelet-activating factor acetylhydrolase Ib (2001), Protein Eng., 14, 513-519.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.47 expression of the alpha1/alpha2 heterodimer or alpha1 homodimer as His-tagged maltose-binding fusion protein in Escherichia coli XL1-Blue using a bi-citronic expression vector Bos taurus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.47 12 mg/ml purified recombinant alpha1/alpha2 heterodimer, hanging-drop vapour diffusion method, 21°C, 17% PEG-MME 2000, 0.1 M sodium acetate, pH 6.4, 10 mM CaCl2, X-ray diffraction structure determination and analysis at resolution 2.1 A Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.47 intracellular
-
 Bos taurus 5622
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.47 additional information Bos taurus enzyme plays a critical role in control of neuronal migration during cortex development ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.47 Bos taurus Q29460 isozyme Ib
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.47 His-tagged alpha1/alpha2 heterodimer or alpha1 homodimer as maltose-binding fusion proteins from Escherichia coli XL1-Blue, removal of tags Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.47 brain
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.47 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O i.e. platelet-activation factor Bos taurus 1-alkyl-sn-glycero-3-phosphocholine + acetate
-
 ?
3.1.1.47 additional information enzyme plays a critical role in control of neuronal migration during cortex development Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.47 More the enzyme exists as a trimer of a beta-subunit and a dimer of alpha-subunits, which can consist of a homodimer of 2 alpha1 or 2 alpha2-subunits or of a alpha1/alpha2 heterodimer Bos taurus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.47 PAF-AH
-
 Bos taurus
3.1.1.47 platelet-activating factor acetylhydrolase
-
 Bos taurus