EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | hexadecyl trimethyl ammonium bromide | strong activation | Aspergillus carneus | |
3.1.1.3 | additional information | no effect by metal chelators | Aspergillus carneus | |
3.1.1.3 | n-octyl-alpha-D-glucopyranoside | strong activation | Aspergillus carneus | |
3.1.1.3 | n-octyl-beta-D-glucopyranoside | strong activation | Aspergillus carneus | |
3.1.1.3 | taurocholic acid | strong activation, 2.7fold at 10 mM | Aspergillus carneus |
EC Number | General Stability | Organism |
---|---|---|
3.1.1.3 | half-life of the enzyme is enhanced by glycine, sorbitol, glycerol, glucose, and ammonium chloride | Aspergillus carneus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | Bromosuccinimide | - |
Aspergillus carneus | |
3.1.1.3 | cholic acid | - |
Aspergillus carneus | |
3.1.1.3 | diethyl 4-nitrophenyl phosphate | strong inhibition | Aspergillus carneus | |
3.1.1.3 | additional information | no effect by metal chelators, no inhibition by trinitrobenzene sulfonic acid, N-ethylmaleimide, p-chloromercuribenzoate, o-phenanthroline, and 4,4'-dipyridyl-disulfide | Aspergillus carneus | |
3.1.1.3 | N-lauroyl sarcosine | strong inhibition | Aspergillus carneus | |
3.1.1.3 | PMSF | strong inhibition | Aspergillus carneus | |
3.1.1.3 | SDS | strong inhibition | Aspergillus carneus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.3 | additional information | - |
additional information | enzyme follows first order reaction kinetics during inactivation | Aspergillus carneus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | Mg2+ | acivation | Aspergillus carneus | |
3.1.1.3 | Na+ | acivation | Aspergillus carneus | |
3.1.1.3 | NH4+ | acivation | Aspergillus carneus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 27000 | - |
about, native PAGE | Aspergillus carneus |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.1.1.3 | Butanol | 80% loss of activity after 30 min, complete loss of activity after 24 h | Aspergillus carneus |
3.1.1.3 | Ethanol | complete loss of activity after 30 min in 90% ethanol, loss of 41% activity after 30 min in 40% ethanol | Aspergillus carneus |
3.1.1.3 | additional information | the enzyme is highly active in presence of many different organic solvents, overview | Aspergillus carneus |
3.1.1.3 | propan-2-ol | 97% loss of activity after 30 min in 60% propan-2-ol, loss of only 3% activity after 30 min in 30% propan-2-ol | Aspergillus carneus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.3 | Aspergillus carneus | - |
alkaline thermostable lipase | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.1.3 | glycoprotein | 12.3% glycosylated | Aspergillus carneus |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.3 | 24.1fold | Aspergillus carneus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.1.1.3 | triacylglycerol + H2O = diacylglycerol + a carboxylate | a tryptophan residue plays an important role in maintaining the ative conformation of the enzyme | Aspergillus carneus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 502 | - |
purified enzyme | Aspergillus carneus |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.1.3 | 22°C, room temperature, lyophilized enzyme, completely stable for more than 1 year | Aspergillus carneus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.3 | 4-nitrophenyl acetate + H2O | low activity | Aspergillus carneus | 4-nitrophenol + acetate | - |
? | |
3.1.1.3 | 4-nitrophenyl caproate + H2O | - |
Aspergillus carneus | 4-nitrophenol + caproate | - |
? | |
3.1.1.3 | 4-nitrophenyl laurate + H2O | enzyme shows a preference for lauric acid and a 1,3-position specificity | Aspergillus carneus | 4-nitrophenol + laurate | - |
? | |
3.1.1.3 | 4-nitrophenyl myristate + H2O | - |
Aspergillus carneus | 4-nitrophenol + myristate | - |
? | |
3.1.1.3 | 4-nitrophenyl palmitate + H2O | - |
Aspergillus carneus | 4-nitrophenol + palmitate | - |
? | |
3.1.1.3 | 4-nitrophenyl stearate + H2O | low activity | Aspergillus carneus | 4-nitrophenol + stearate | - |
? | |
3.1.1.3 | additional information | the enzyme utilizes olive oil as a substrate, no activity with 2-nitrophenyl palmitate | Aspergillus carneus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.3 | monomer | 1 * 27000, SDS-PAGE | Aspergillus carneus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.3 | lipase | - |
Aspergillus carneus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 37 | - |
- |
Aspergillus carneus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 5 | 90 | - |
Aspergillus carneus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | additional information | - |
thermostability is enhanced by glycine, sorbitol, glycerol, glucose, and ammonium chloride | Aspergillus carneus |
3.1.1.3 | 70 | - |
5 min, stable, after 10 min enzyme shows loss of 45% activity | Aspergillus carneus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 9 | - |
- |
Aspergillus carneus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.1.3 | Aspergillus carneus | isoelectric focusing | 4.4 | 4.3 |