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Literature summary extracted from

  • Lee, Y.C.; Martin, E.; Murad, F.
    Human recombinant soluble guanylyl cyclase: expression, purification, and regulation (2000), Proc. Natl. Acad. Sci. USA, 97, 10763-10768.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.6.1.2 3-(5'-hydroxymethyl-2'furyl)-1-benzyl-indazole 0.1 mM, approx. 50fold stimulation, more than 2000fold stimulation in the presence of 0.1 mM sodium prusside Homo sapiens
4.6.1.2 NO
-
 Homo sapiens
4.6.1.2 sodium nitroprusside 0.1 mM, 580fold stimulation, NO donor Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.2 expression of alpha1 and beta1 subunits of sGC in Sf9 insect cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.2 1H-(1,2,4)oxadiazole(4,3-a)quinoxalin-1one 0.1 mM, complete inhibition Homo sapiens
4.6.1.2 3',5'-cyclic GMP 20 mM, 20-30% competitive inhibition Homo sapiens
4.6.1.2 diphosphate competitive inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.2 0.065
-
 GTP recombinant sGC Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.6.1.2 soluble
-
 Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.6.1.2 155000
-
 gel filtration, recombinant His-tagged sGC Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.2 GTP Homo sapiens
-
 3',5'-cyclic GMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.2 Homo sapiens Q02108
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.2 recombinant sGC Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.2 0.0056
-
 basal sGC activity Homo sapiens
4.6.1.2 3.25
-
 sodium nitroprusside activated sGC activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.2 GTP
-
 Homo sapiens 3',5'-cyclic GMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.6.1.2 dimer alpha1,beta1, 0.9 equivalents of heme per sGC subunit Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.6.1.2 sGC
-
 Homo sapiens
4.6.1.2 soluble guanylyl cyclase
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.6.1.2 0.3
-
 diphosphate
-
 Homo sapiens