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Literature summary extracted from

  • Menand, B.; Marechal-Drouard, L.; Sakamoto, W.; Dietrich, A.; Wintz, H.
    A single gene of chloroplast origin codes for mitochondrial and chloroplastic methionyl-tRNA synthetase in Arabidopsis thaliana (1998), Proc. Natl. Acad. Sci. USA, 95, 11014-11019.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.10 DNA determination and analysis, overexpression in Escherichia coli SG13009, in vitro translation, expression of the enzyme as GFP-fusion protein in Nicotiana tabacum suspension cells Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.10 chloroplast a single gene of chloroplast origin codes for mitochondrial and chloroplastic isozyme, possessing a targeting signal sequence with dual function Arabidopsis thaliana 9507
-
6.1.1.10 chloroplast the isozyme from chloroplasts is identical with the one from mitochondria Pisum sativum 9507
-
6.1.1.10 cytoplasm cytoplasmic enzyme form Pisum sativum 5737
-
6.1.1.10 mitochondrion a single gene of chloroplast origin codes for mitochondrial and chloroplastic isozyme, possessing a targeting signal sequence with dual function Arabidopsis thaliana 5739
-
6.1.1.10 mitochondrion the isozyme from mitochondria is identical with the one from chloroplasts Pisum sativum 5739
-
6.1.1.10 additional information the in vitro synthesized enzyme is taken up by specific transport into chloroplasts of Pisum sativum, and in mitochondria of Solanum tuberosum, the imported protein becomes processed in both cases Arabidopsis thaliana
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.10 62000
-
 x * 62000, processed enzyme, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet Pisum sativum
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet Arabidopsis thaliana
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.10 Arabidopsis thaliana
-
 a single gene of chloroplast origin codes for mitochondrial and chloroplastic isozyme
-
6.1.1.10 Arabidopsis thaliana O23761 a single gene of chloroplast origin codes for mitochondrial and chloroplastic isozyme
-
6.1.1.10 Arabidopsis thaliana Q9M2T9 a single gene of chloroplast origin codes for mitochondrial and chloroplastic isozyme
-
6.1.1.10 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.10 enzymes from chloroplasts and mitochondria, which are indistinguishable in the purificaion process, partial Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.10 leaf
-
 Pisum sativum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Pisum sativum AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Arabidopsis thaliana AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.10 ? x * 62000, processed enzyme, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
6.1.1.10 Methionine translase
-
 Pisum sativum
6.1.1.10 Methionine translase
-
 Arabidopsis thaliana
6.1.1.10 Methionine--tRNA ligase
-
 Pisum sativum
6.1.1.10 Methionine--tRNA ligase
-
 Arabidopsis thaliana
6.1.1.10 Methionyl tRNA synthetase
-
 Pisum sativum
6.1.1.10 Methionyl tRNA synthetase
-
 Arabidopsis thaliana
6.1.1.10 Methionyl-transfer ribonucleate synthetase
-
 Pisum sativum
6.1.1.10 Methionyl-transfer ribonucleate synthetase
-
 Arabidopsis thaliana
6.1.1.10 Methionyl-transfer ribonucleic acid synthetase
-
 Pisum sativum
6.1.1.10 Methionyl-transfer ribonucleic acid synthetase
-
 Arabidopsis thaliana
6.1.1.10 Methionyl-transfer RNA synthetase
-
 Pisum sativum
6.1.1.10 Methionyl-transfer RNA synthetase
-
 Arabidopsis thaliana
6.1.1.10 MetRS
-
 Pisum sativum
6.1.1.10 MetRS
-
 Arabidopsis thaliana
6.1.1.10 Synthetase, methionyl-transfer ribonucleate
-
 Pisum sativum
6.1.1.10 Synthetase, methionyl-transfer ribonucleate
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.10 ATP
-
 Pisum sativum
6.1.1.10 ATP
-
 Arabidopsis thaliana