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Literature summary extracted from

  • Rose, I.A.; Weaver, T.M.
    The role of the allosteric B site in the fumarase reaction (2004), Proc. Natl. Acad. Sci. USA, 101, 3393-3397.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.2 H129N loss of D2O inhibitory effect, product release step is accelerated by glycerol compared to inhibition of wild-type enzyme. 3.1fold reduced maximal velococity in reaction with malate, 1.13fold reduced maximal velocity in reaction with fumarate compared to wild-type enzyme Escherichia coli
4.2.1.2 K127D mutant enzyme behaves like wild-type enzyme Escherichia coli
4.2.1.2 R126A loss of D2O inhibitory effect, product release step is accelerated by glycerol compared to inhibition of wild-type enzyme. 4.3fold reduced maximal velococity in reaction with malate, 2.7fold reduced maximal velocity in reaction with fumarate compared to wild-type enzyme Escherichia coli
4.2.1.2 R126A/H129N great loss of D2O inhibitory effect, product release step is accelerated by glycerol compared to inhibition of wild-type enzyme. 8.6fold reduced maximal velococity in reaction with malate, 7.1fold reduced maximal velocity in reaction with fumarate compared to wild-type enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.2 glycerol wild-type enzyme is inhibited due to a viscogenic effect on the recycling rate Escherichia coli
4.2.1.2 meso-tartrate
-
 Escherichia coli
4.2.1.2 additional information inhibition by D2O arises in the recycling phase Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.2 Escherichia coli
-
 FumC
-
4.2.1.2 Escherichia coli FumC
-
 FumC
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.2 (S)-malate
-
 Escherichia coli fumarate + H2O wild-type enzyme is rate-limited in the recycling of free enzyme isoforms that follows product release ?
4.2.1.2 (S)-malate
-
 Escherichia coli FumC fumarate + H2O wild-type enzyme is rate-limited in the recycling of free enzyme isoforms that follows product release ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.2 FumC
-
 Escherichia coli