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Literature summary extracted from

  • Lupidi, G.; Venardi, G.; Bollettini, M.; Marmocchi, F.; Rotilio, G.
    Purification and partial characterization of glyoxalase I from bovine brain (2001), Prep. Biochem. Biotechnol., 31, 305-316.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.5 1,10-phenanthroline 2 mM, approx. 50% inactivation after 10 min, almost complete inhibition after 180 min Bos taurus
4.4.1.5 2,6-pyridindicarboxylic acid 2 mM, approx. 50% inactivation after 10 min, almost complete inhibition after 180 min Bos taurus
4.4.1.5 EDTA 0.1 mM, approx. 50% inactivation after 50 min, approx. 80% inhibition after 250 min Bos taurus
4.4.1.5 S-bromobenzylglutathione
-
 Bos taurus
4.4.1.5 S-bromobenzylglutathione
-
 Saccharomyces cerevisiae
4.4.1.5 S-p-nitrobenzylglutathione
-
 Bos taurus
4.4.1.5 S-p-nitrobenzylglutathione
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.5 0.29
-
 glutathione-methylglyoxal hemithioacetal
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.5 Mg2+ incubation of the Zn2+ depleted apoenzyme with Mg2+ restores 50% of the enzyme activity Bos taurus
4.4.1.5 Zn2+ 1 mol Zn2+ per subunit Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.5 22000
-
 2 * 22000, SDS-PAGE Bos taurus
4.4.1.5 44000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.5 glutathione-methylglyoxal hemithioacetal Saccharomyces cerevisiae glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione (R)-S-lactoylglutathione
-
 ?
4.4.1.5 glutathione-methylglyoxal hemithioacetal Bos taurus glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione (R)-S-lactoylglutathione
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.5 Bos taurus
-
-
-
4.4.1.5 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.5
-
 Saccharomyces cerevisiae
4.4.1.5 60°C, DEAE-52 cellulose, S-hexylglutathione Sepharose 6 B affinity, gel filtraton Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.5 425
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.5 glutathione-methylglyoxal hemithioacetal glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione Saccharomyces cerevisiae (R)-S-lactoylglutathione
-
 ?
4.4.1.5 glutathione-methylglyoxal hemithioacetal glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione Bos taurus (R)-S-lactoylglutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.5 dimer 2 * 22000, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
4.4.1.5 glyoxalase I
-
 Saccharomyces cerevisiae
4.4.1.5 glyoxalase I
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.4.1.5 0.0057
-
 S-bromobenzylglutathione
-
 Saccharomyces cerevisiae
4.4.1.5 0.021
-
 S-bromobenzylglutathione
-
 Bos taurus
4.4.1.5 0.032
-
 S-p-nitrobenzylglutathione
-
 Bos taurus
4.4.1.5 0.083
-
 S-p-nitrobenzylglutathione
-
 Saccharomyces cerevisiae