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Literature summary extracted from

  • Cumino, A.; Ekeroth, C.; Salerno, G.L.
    Sucrose-phosphate phosphatase from Anabaena sp. strain PCC 7120: isolation of the protein and gene revealed significant structural differences from the higher-plant enzyme (2001), Planta, 214, 250-256.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.24 DNA and amino acid sequence determination and analysis, expression in Escherichia coli DH5alpha as His-tagged enzyme Anabaena sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.24 fluoride 5% inhibition at 2.5 mM Anabaena sp.
3.1.3.24 molybdate 12% inhibition at 2.5 mM Anabaena sp.
3.1.3.24 sucrose weak competitive product inhibition Anabaena sp.
3.1.3.24 vanadate 50% inhibition at 2.5 mM Anabaena sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.24 0.35
-
 sucrose 6-phosphate
-
 Anabaena sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.24 Mg2+
-
 Anabaena sp.
3.1.3.24 Mn2+
-
 Anabaena sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.24 26000 27000 native enzyme, gel filtration Anabaena sp.
3.1.3.24 28000
-
 1 * 28000, native enzyme, SDS-PAGE, 1 * 28034, native enzyme, amino acid sequence calculation, 1 * 32000, recombinant His-tagged enzyme, SDS-PAGE Anabaena sp.
3.1.3.24 28034
-
 1 * 28000, native enzyme, SDS-PAGE, 1 * 28034, native enzyme, amino acid sequence calculation, 1 * 32000, recombinant His-tagged enzyme, SDS-PAGE Anabaena sp.
3.1.3.24 32000
-
 1 * 28000, native enzyme, SDS-PAGE, 1 * 28034, native enzyme, amino acid sequence calculation, 1 * 32000, recombinant His-tagged enzyme, SDS-PAGE Anabaena sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.24 sucrose 6-phosphate + H2O Anabaena sp.
-
 sucrose + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.24 Anabaena sp. Q93M81 strain PCC 7120
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.24 recombinant enzyme from Escherichia coli partially, and native for strain PCC 7120 3260fold Anabaena sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.24 15.66
-
 purified enzyme Anabaena sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.24 additional information no activity with glucose 1-phosphate, fructose 6-phosphate, fructose 1,6-bisphosphate, glucose 1,6-bisphosphate, and trehalose 6-phosphate Anabaena sp. ?
-
 ?
3.1.3.24 sucrose 6-phosphate + H2O
-
 Anabaena sp. sucrose + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.24 monomer 1 * 28000, native enzyme, SDS-PAGE, 1 * 28034, native enzyme, amino acid sequence calculation, 1 * 32000, recombinant His-tagged enzyme, SDS-PAGE Anabaena sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.3.24 sucrose-phosphate phosphatase
-
 Anabaena sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.24 6.5
-
-
 Anabaena sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.24 80
-
 sucrose
-
 Anabaena sp.