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Literature summary extracted from

  • El-Shora, H.M.
    Properties of phenylalanine ammonia-lyase from marrow cotyledons (2002), Plant Sci., 162, 1-7.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.3.1.24 NaCl maximal activity at 200 mM, inhibition above Cucurbita pepo

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.24 ADP 5 mM, approx. 50% inhibition Cucurbita pepo
4.3.1.24 AMP 5 mM, approx. 40% inhibition Cucurbita pepo
4.3.1.24 ATP 5 mM, approx. 80% inhibition Cucurbita pepo
4.3.1.24 beta-chloroethyltrimethylammonium 10 mM, 60% inhibition of PAL activity in cotyledons Cucurbita pepo
4.3.1.24 Ca2+ 6 mM, 40% inhibition Cucurbita pepo
4.3.1.24 EDTA 35 mM, complete inactivation Cucurbita pepo
4.3.1.24 N,N-Dicyclohexylcarbodiimide 10 mM, 82% inhibition Cucurbita pepo
4.3.1.24 N-acetylimidazol 10 mM, 75% inhibition Cucurbita pepo
4.3.1.24 o-phenanthroline 20 mM, complete inactivation Cucurbita pepo
4.3.1.24 Phenylmethylsulphonyl fluoride 10 mM, 59% inhibition Cucurbita pepo

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.24 Mg2+ approx. 3fold activation at 8 mM Cucurbita pepo
4.3.1.24 Mn2+ approx. 1.5fold activation at 8 mM Cucurbita pepo

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.24 L-phenylalanine Cucurbita pepo first enzyme in the phenylpropanoid biosynthetic pathway (E)-cinnamate + NH3
-
 r

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
4.3.1.24 Glycerol stabilizes Cucurbita pepo

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.24 Cucurbita pepo
-
 marrow
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.24 ammonium sulfate, DEAE-cellulose, Sephadex G-100, Q-Sepharose Cucurbita pepo

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.1.24 cotyledon
-
 Cucurbita pepo
-
4.3.1.24 seed
-
 Cucurbita pepo
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.24 26.6
-
-
 Cucurbita pepo

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.24 L-phenylalanine
-
 Cucurbita pepo (E)-cinnamate + NH3
-
 ?
4.3.1.24 L-phenylalanine first enzyme in the phenylpropanoid biosynthetic pathway Cucurbita pepo (E)-cinnamate + NH3
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.3.1.24 PAL
-
 Cucurbita pepo

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.1.24 5
-
 40% and 90% loss of activity after 1 and 5 days, respectively Cucurbita pepo
4.3.1.24 5
-
 approx. 30% loss of activity after 5 days in the presence of 30% glycerol Cucurbita pepo