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Literature summary extracted from

  • Jang, I.C.; Oh, S.J.; Seo, J.S.; Choi, W.B.; Song, S.I.; Kim, C.H.; Kim, Y.S.; Seo, H.S.; Choi, Y.D.; Nahm, B.H.; Kim, J.K.
    Expression of a bifunctional fusion of the Escherichia coli genes for trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase in transgenic rice plants increases trehalose accumulation and abiotic stress tolerance without stunting growth (2003), Plant Physiol., 131, 516-524.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.3.12 biotechnology The transformation of rice plants with a gene that encodes a bifunctional fusion enzyme of trehalose-6-phosphate synthase and – phosphatase from Escherischia coli increases the trehalose levels in these plants, shows no visible growth inhibition. The production of trehalose in these plants results in increased tolerance to drought, salt, and cold stresses. Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.15 functional expression of the enzyme as a fusion protein with Escherichia coli trehalose-6-phosphate phosphatase in transgenic Oryza sativa plants Escherichia coli
3.1.3.12 commentary Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.15 additional information construction of transgenic Oryza sativa plants expressing the catalytically active enzyme as a fusion protein with trehalose-6-phosphate phosphatase of Escherichia coli under control of the ubiquitin promotor from Zea mays, the transgenic plants produce high levels of trehalose in seeds and leaves, carbohydrate profile of seeds, but not of leaves, is altered, plants show no growth inhibition or altered phenotype Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.12 973000
-
 calculated from amino acid sequence Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.15 additional information Escherichia coli trehalose acts as a global protectant against abiotic stress, accumulation of trehalose leads to increased salt, drought, and cold resistance in plants ?
-
 ?
3.1.3.12 trehalose 6-phosphate + H2O Escherichia coli the product trehalose acts as a global against abiotic stress trehalose + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.15 Escherichia coli
-
-
-
3.1.3.12 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.15 additional information trehalose acts as a global protectant against abiotic stress, accumulation of trehalose leads to increased salt, drought, and cold resistance in plants Escherichia coli ?
-
 ?
3.1.3.12 trehalose 6-phosphate + H2O
-
 Escherichia coli trehalose + phosphate
-
 ?
3.1.3.12 trehalose 6-phosphate + H2O the product trehalose acts as a global against abiotic stress Escherichia coli trehalose + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.15 T-6-P
-
 Escherichia coli
2.4.1.15 trehalose-6-phosphate synthase
-
 Escherichia coli